HCF-av人心脏成纤维细胞哪里有-成人心室

HCF-av人心脏成纤维细胞-成人心室
货号：GD-C100318380
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
造成实验室细胞污染常见情况总结：细胞培养中Z常见污染的是细菌、真菌和支原体污染。细胞一旦污染，大多数较难处理。那么，哪些情况我们不注意的话就会造成细胞污染呢？我们根据常见细胞培养实验分析总结下。【违规操作】1)为节省时间，有人已经用超净台四个多小时，不开紫外灭菌30min,酒精擦拭后直接开始试验；2)器材或者溶液很久没用，未检测是否污染而直接使用；离心管多次使用，枪头为了方便交叉使用；3)超净台不点酒精灯；点了酒精灯放在右上角，而你在左下角做试验；4)不带手套，徒手操作；5)细胞培养间配备枪式移液器、手术器械、离心机、冰箱等专用仪器设备以及专用的实验服和拖鞋，未定期消毒。专用物品被带出传代细胞使用。培养细胞过程中使用的所有实验用具，如移液管、一次性枪头、一次性塑料离心管、冻存管等未按要求灭菌使用(通常需121°C高压灭菌20分钟后37％烤干备用)。超净台和桌面，东西太多太乱：超净台不是储物箱，什么培养皿、各种规格的板子、枪头就不要堆在超净台！这样就会有许多紫外线顾不到的卫生死角。传代细胞其他的桌面，切忌东西堆积如山，不要将酒精棉球、标签纸、牛皮纸买来后全部堆在传代细胞！一不小心“飘”进你的细胞培养板里，细胞就会养的不好，啥时候死了都不知道！【培养箱太久没清洁】细胞污染了，并非直接扔了培养皿就不管了，首先你还得看看这个恒温培养箱里其他培养皿或孔板里的细胞是否污染，如果有而且好几个板子都有类似的污染块，那很可能是培养箱中的水或者空气污染了，得给培养箱做个大扫除，重新酒精消毒，照紫外；孵箱里的水，水没了要记得加，还得记得十天半个月的就用酒精擦擦托盘。【传代细胞人多口杂，难管理】在传代细胞这种卫生要求高，人多了，不确定因素多了，难以保证试验在无菌条件下操作。出入试验室，实验服当风衣穿，不扣纽扣，不戴鞋套，就容易造成细胞污染；超净台做实验时，喜欢说话聊着做试验，要是还不带口罩，里面就有很多细菌等着去攻击你的细胞呢！
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(S)-(+)-1-Benzyl-3-hydroxypiperidine, 97%
L-Cysteine hydrochloride,anhydrous
2,5-Dibenzylidenecyclopentanone , 98%
2,5-Difluorobenzoic acid
3,5-Difluoro-2-MethoxybenzylaMine, 97%
4-Isopropoxy-2-(trifluoroMethyl)phenylboronic acid
Ethyl Maltol
2-Chloropyridine-4-carboxylic acid
3-Methyl-2-benzoxazolinone, 98%
4-(Aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol
N-Glycyl-DL-2-aminobutyric Acid
1-N-CBZ-PYRROLIDINE-3-CARBOXYLIC ACID
4-Methyl-1,2,4-triazole-3-thiol
5-METHYL-1,3,4-OXADIAZOL-2-YLAMINE
Di-p-anisoyl-D-tartaric acid
4-(4-Iodobenzyl)Morpholine
1,1'-Di-n-octyl-4,4'-bipyridiniuM DibroMide
6-BroMo-3-Methylindazole
Propargyl alcohol, 99%
Guar GuM, NF
Phenylthiohydantoin-norleucine
Norharman
1-methoxy-2-(2-nitrovinyl)benzene
tetramethylammonium hexafluorophosphate
m-xylene-4-sulphonic acid
3,3-DIMETHYLGLUTARIC ANHYDRIDE
5-AMino-2-hydroxypyridine

L-ValinaMide hydrochloride, 95%
5-Chloro-4-azaindole-2-carboxylic acid, 98%

Tri-n-octyltin Hydride [Reducing Reagent], 95%
3-HEPTENOIC ACID
TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) CHLORIDE HEXAHYDRATE
Boc-beta-alanine
4-Iodo-1-Methylpyrazole-5-carboxylic Acid , 98%
1,8-Bis(hydroxymethyl)anthracene
4-Mercapto-1H-pyrazolo[3,4-d]pyrimidine hemihydrate, 99%
5-Acetyl-3-chloro-10,11-dihydrodibenzo[b,f]azepine
1-Oxa-9-azaspiro[5.5]undecane
2-BROMO-5-METHOXYBENZOYL CHLORIDE
6-CHLORO-4-HYDROXYCOUMARIN
Dodecylheptaglycol
Pentacene
Adenosine 5'-diphosphate
3-Chloro-L-alanine Hydrochloride
1-Propyl-1H-pyrazole-5-boronic acid pinacol ester
Hydroxypropyl cellulose, Average M.W. 100.000
N-Octyl isothiocyanate, 95%
3-AMinocyclohexanecarboxylic Acid (cis- and trans- Mixture)
2-Fluoro-5-(trifluoroMethyl)phenylboronic Acid (contains varying aMounts of Anhydride)
3,4'-DIMETHYLBENZOPHENONE
Phenyl ether
1-BOC-ISONIPECOTIC ACID HYDRAZIDE
Diacetato[(R)-(+)-2,2'-bis(di-p-tolylphosphino)-1,1'-binaphthyl]ruthenium(II)
1-Benzyl-2,5-dihydro-1H-pyrrole
FMoc-Nva-OH
N-PHENYLMALEAMIC ACID
3-(ETHYLAMINO)PYRROLIDINE
5-Fluoro-2-methylbenzoxazole
n-Octylmethyldichlorosilane
(R)-(-)-1-Octen-3-ol, 99%
Propylene Glycol Monostearate (contains ca. 35% Monopalmitate)
4,8-DICHLOROQUINOLINE
6-FLUOROGRAMINE
ALPHA-METHYLBENZYL ALCOHOL
Cyanine;Quinoline blue; 1,1′-DiisoaMyl-4,4′-cyanine iodide; Bis-(1-isoaMylchinoline-4)Methincyanine iodide;
PotassiuM vinyltrifluoroborate
(2-BroMoethyl)diphenylsulfoniuM TrifluoroMethanesulfonate
4-Fluorophenylboronic acid, pinacol ester
DiMethyl isophthalate, 99%
4-BenzyloxyphenylMagnesiuM broMide, 0.8M solution in THF, AcroSeal
2-AMino-5-broMo-4-Methylpyridine
Maltohexaose
2-Pyridinecarboxylic acid, 3-amino-
2-Chloro-4-methoxy-pyrimidine-5-carbonitrile
(R)-3-hydroxymethyl-pyrrolidine-1-carboxylic acid Tert-Butyl ester
3-Chloro-5-fluorobenzaldehyde
CDK5 ELISA Kit
HCV IgA ELISA Kit
TP ELISA Kit
Human GAD65 ELISA Kit
IgA ELISA Kit
17-OHP ELISA Kit
IGFBP3 ELISA Kit
Human LPA ELISA Kit
Human CCNI2 ELISA Kit
OVA sIgG ELISA Kit
Motility-Indol-Urea Medium Base
Holley-Baker Medium
Capryl Alcohol
Manganese(II) naphthenate, 56% w/w in Mineral spirits, 6-10% Mn
5-Nitro-2-furoyl chloride, 97%
2-Hydroxy-4-Picoline
Norharman
Basic Violet 16
3,5-dimethylcyclohexanol
DL-Menthol

1,4-DICHLORO-2-IODOBENZENE
D-ARABINITOL
2-(N-Morpholino)Ethanesulfonic Acid PotassiuM Salt
N-BsMoc-L-asparagine, 98%
1-Methylpiperidine, 99%
Isoquinoline-3-carboxylic Acid Monohydrate
PotassiuM persulfate
(+)-EPI-QUERCITOL
6-BroMo-4-chloroquinoline
FMoc-Tyr(tBu)-OPfp
Hesperidin Methyl chalcone
2,5-Dichloronicotinic acid
3-Ethoxyphenylboronic acid ,98%
(1R,2R)-N,N-Bis{2-[bis(3,5-dimethylphenyl)phosphino]benzyl}cyclohexane-1,2-diamine, min. 97%
Boc-Dap(Fmoc)-OH