MPC-11细胞系：小鼠浆细胞株 包售后 货号：GD-C4119041
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1-BroMo-4-(n-octyloxy)benzene, 98%
Dextrin
Methyl 3-(benzyloxy)-4-broMobenzoate
N-Alpha-t-Boc-D-aspartic acid beta-benzyl ester
2-Chloro-3-Methoxybenzonitrile
3-AMinocarbonylphenylboronic acid, pinacol ester
TERT-BUTYL ISOTHIOCYANATE
3,3-Dimethoxy-2-butanone, 98%
1,2-Dichlorobenzene D4 >99%
2,3-Dimethyl-1-pentene
4-Nitrophenol Sodium Salt Dihydrate
5,5-Dimethyloxazolidine-2,4-dione
1-ETHYL-7-CHLORO-6-FLUORO-1,4-DIHYDRO-4-OXOQUINOLINE-3-CARBOXYLIC ACID
1,3-DIISOPROPENYLBENZENE
Butvar B-98
L(-)-Glucose
DodecyltriMethoxysilane
Ethyl 4-broMo-3-Methylbenzoate
Triethylene glycol, 99%
PotassiuM Pyrophosphate, Powder, FCC
Bis(tri-tert-butylphosphine)platinuM(0)
2-Chlorobenzamide
2,3-Dimethoxybenzonitrile
ethyl 2-methyl-1H-pyrrole-3-carboxylate
methyl trifluoromethanesulphonate
CROCONIC ACID
COBALT(II) PHOSPHATE OCTAHYDRATE
Zinc Stearate
Fluphenazine dihydrochloride, 98%
1,4-Bis(4-pyridylMethyl)benzene, 97%
Cyhalofop-butyl
Fluazuron
1-Isocyanato-4-Methoxy-2-nitrobenzene
PotassiuM cyanate
N-UNDECYL ETHER
2-Acetylpyridine
4-Amino-3-methylbenzoic acid
6-Chloro-7-deazapurine ,98%
2,6-Dichloropyrimidine-4-carboxylic acid
Phenethyl-hydrazine
2-Bromo-2-methylpropionic acid
4-Methyl-benzoic acid tert-butyl ester
3-FLUORO-2-HYDROXYPYRIDINE
HexaMethylenetetraMine;Urotropine;Accelerator H;AMinoforM;AMMoforM;MethenaMine;CystaMin;Cystogen;1,3,5,7-TetraazaadaMantane
2-BroMo-5-chloro-3-Methylbenzo[b]thiophene
Benzofuran-3(2H)-one
N,N'-MethylenebisforMaMide
N-Methyl-4-(MethylaMino)picolinaMide
2-Acetylbutyrolactone, 99+%
H-D-LYS(Z)-OH
L-(+)-Gulonic Acid gaMMa-Lactone
gastrin I
BOC-4-HYDROXY-D-PHENYLGLYCINE
Phosphorouspentabromide
2-Octanol
2,3-HEXANEDIONE
4,5-DibroMo-1-trityl-1H-iMidazole
BenzyldodecyldiMethylaMMoniuM broMide
2-TrifluoroMethyl-5-broMopyridine
Boc-gamma-Abu-OH
2,4,5-Trichlorophenyl isothiocyanate
Boc-DL-cyclopentylglycine
1-(1-methyl-1H-benzimidazol-2-yl)ethanone(SALTDATA: FREE)
4,4'-Dichlorobenzophenone
2,6-Naphthalenediol diacetate
YTTRIUM NITRATE HEXAHYDRATE
Orbifloxacin
1-Phenylcyclobutanecarbonitrile
1,5-Diphenyl-3-(p-tolyl)forMazan
4-BroMoacetyl-3-fluorophenylboronic acid
5-BroMo-2-Methylaniline, 98%
2,4-Difluorobenzene-1-carbothioaMide, 97%
SODIUM XYLENESULFONATE
1-CyanoadaMantane
Triphenylmethane
(R)-2-N-Boc-aminomethylmorpholine
boc-L-leucinol
3-Iodobenzoic acid ethyl ester
CYFRA21.1 ELISA Kit
α-SMA ELISA Kit
Human MUC21 ELISA Kit
Human Chlamydia pneumoniae antibody,Cpn-Ab ELISA Kit
PGC-1α ELISA Kit
S-100 ELISA Kit
Chicken Interleukin 2,IL-2 ELISA Kit
Human growth hormone releasing hormone，GHRH ELISA Kit
VEGF-A ELISA Kit
STAT3 ELISA Kit
Fastidious Anaerobe Broth (FAB)
Yeast Synthetic Drop-out Agar Medium without Uraci
IsobutyraMide, 99%
N,N'-DIMETHYLTHIOUREA
5-Chlorobenzo[b]thiophene-3-acetonitrile, 97%
2-Fluoroanisole
2-Chlorobenzamide
Bambermycin
Calcium Iodate
2,6-Difluorobenzoic acid
N-PENTYL-4-AMINOBENZOATE
2-THIOPHENECARBOXYLIC ACID SODIUM SALT
SILVER TRIFLUOROMETHANESULFONATE
3-Fluoro-2-Methylbenzonitrile, 97%
6-Nitroquinoline, 98+%
2,6-Difluorobenzyl alcohol
1-MethylpyridiniuM Chloride
(-)-1,4-DI-O-TOSYL-2,3-O-ISOPROPYLIDENETHREITOL
5-BROMO-2-CHLOROANISOLE
Diethyl azodicarboxylate
1-OCTADECYL-4-(4-PHENYL-1,3-BUTADIENYL)PYRIDINIUM BROMIDE
2-(1H-Benzo[d]imidazol-2-yl)aniline , 97%
3,4,6-Tri-O-acetyl-2-deoxy-D-galactopyranose
3,3-Difluoropyrrolidine hydrochloride
1-Acetamido-4-bromonaphthalene 98%
