IAR-20细胞系：小鼠肝细胞株 技术硬 货号：GD-C3920074
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
细胞培养常见问题及解决方案:1)培养瓶有破裂，培养液有漏液：细胞极大可能会污染，所以我们会及时安排帮老师解决；2)细胞漂浮：培养瓶不开封，瓶口酒精擦拭后平躺放置在培养箱。次日观察，如细胞大部分又贴回瓶底，表明细胞活力正常，剩余漂浮的细胞可以离心去掉，留10ml培养液培养观察，细胞生长至汇合度80%，进行消化传代；如细胞还是不贴壁，将细胞离心收集转到新培养瓶，原培养瓶加部分培养液继续培养，中间注意观察，我们的技术人员会一直跟踪指导，直到问题解决；3)对于生长缓慢的贴壁细胞：可采用适当的提高培养基中血清浓度，或隔日换液的方法来保证细胞的状态和生长速度；4)对于生长不均的贴壁细胞：在培养过程中若出现细胞分布明显不均时(即某一区域细胞已重叠生长，而旁边则为一块空白)，此时可将细胞进行消化，重新打散，贴壁，加入新培养基进行培养。
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
o-PhenylenediaMine, 98%
2-Benzothiazoleacetonitrile
3-(3-CYANO-PHENYL)-ACRYLIC ACID METHYL ESTER
Benzyl Methacrylate
Benzotriazole-1-yl-oxytripyrrolidinophosphoniuM hexafluorophosphate
1,4-Diiodobutane
PigMent Yellow 14
1-[5-(Trifluoromethyl)-2-pyridyl]piperidine-4-carboxylic acid , 95%
(5-Chloro-1H-indazol-3-yl)-methanol ,97%
L-Cysteine-glutathione disulphide
3-Carboxy-2-fluorophenylboronic acid
1-(4-Aminophenyl)-4-(4-hydroxyphenyl)
Furfuryl thioformate
Mosapride citrate dihydrate
2-Bromopropionyl chloride
2-Nitrobenzoic Acid
Diisopropyl Sulfate
3,4-DibroMo-2,5-dichlorothiophene
ThyMol Blue, pure, indicator
PalMarosa Oil, FCC
AcetaMidoMethanol
1,2-dibromobenzene
Methyl 3-methoxy-4-nitrobenzoate
1-ethynyl-4-methoxybenzene
4,6-dihydro-4-hydroxy-2H-furo[3,2-c]pyran-2-one
FLUOROTRIBROMOMETHANE
2,4-Dimethoxyacetophenone
1-Methyl-3-piperidineMethanol
4,5-DiMethoxy-2-nitrocinnaMic acid, 98%
PotassiuM carbonate sesquihydrate, ACS, 98.5-101.0%
Di(ethylene glycol) diacrylate
Adipic acid dihydrazide
3-Chloro-3-Methylpentane
Salicylazosulfapyridine
5-(4-Chlorophenyl)isoxazole-3-propionic acid
HESPERETIN
Methyl 4-(bromomethyl)benzoate
2-(Methylcarboxy)pyridine-5-boronic acid pinacol ester
Direct Blue 1
6-Phenyl-2H-pyridazin-3-one
N-alpha-FMOC-Nepsilon-BOC-L-Lysine
4-METHOXYBENZYL MERCAPTAN
2-BROMO-1-(3,4-DIMETHOXYPHENYL)ETHANONE
RHENIUM
Methyl 3-aMino-2-pyrazinecarboxylate
Zinc carbonate basic
4,6-DiiodopyriMidine
3,4-Epoxytetrahydrofuran, 96%
2,2-DiMethyl-N-(4-pyridinyl)propanaMide, 97%
5'-O-Dimethoxytrityl-N-isobutyryl-deoxyguanosine
M-Toluic Acid
Theobromine
3-[ethoxy(dipropan-2-yl)silyl]propan-1-amine
hexadecylphosphocholine
4-Bromophenylisocyanide
1-Indanamine
N-OCTANAMIDE
4-Chloro-2,6-difluorophenylaceticacid
2-Fluorobenzenesulfonyl chloride
5-(Hydroxymethyl)uracil ,98%
3-(4-Methyl-piperazin-1-yl)-propionic acid ethylester
Sucrose monocaprate ,98%
4-Cyanobenzyl-triphenylphosphonium chloride, 98%
3-BROMO-5-FLUOROBENZALDEHYDE
4-CHLORO-2-FLUOROIODOBENZENE
CALCIUM GLUCONATE MONOHYDRATE
2,6-Difluorobenzaldehyde
Methyl sulfone
1,3-Bis(4-Methoxyphenoxy)benzene
3-Butoxy-2,4,6-trifluorophenylboronic acid
alpha-Chloro-p-xylene, 99+%
8-AMinoisoquinoline, 97%
Acid Red 114
RutheniuM(III) Chloride
2-Chloroquinoline
(R)-N-BOC-2-(5-Bromothienyl)alanine
Amines, C20-22-alkyldimethyl
1-(4-Fluorophenyl)-1,3-dihydro-isobenzofuran-5-carbonitrile
TSLP ELISA Kit
bACE1 ELISA Kit
LPL ELISA Kit
Human CPLX3 ELISA Kit
PGF2α ELISA Kit
KEAP1 ELISA Kit
SHBG ELISA Kit
VMA ELISA Kit
BrDU ELISA Kit
VD3 ELISA Kit
Orange Serum Agar
Folic Acid Assay Medium
5,6,7,8,9,10-Hexahydrocyclohept[b]indole, 98%
AMMoniuM chroMate, 98+%
5-AMino-3-(2-thienyl)-1H-pyrazole, 98%
2-Methyl-1-butene, 98%
1,2-dibromobenzene
Cobalt sulfate anhydrous
2,6-diacetyl-1,2,3,9b-tetrahydro-7,9-dihydroxy-8,9b-dimethyldibenzofuran-1,3-dione
Nα-Benzoyl-L-argininie
1-Methoxynaphthalene
Taxifolin
N-Alpha-t-Butyloxycarbonyl-D-Serine
N-(4-BroMobenzyl)-2-fluorobenzaMide, 97%
5-Chloro-1-indanone, 99%
2-Iodo-4-nitroaniline
Trioctylphosphine oxide
DisodiuM Diphenylsulfone-4,4'-dichloro-3,3'-disulfonate
3,6-Dichloro-4-Methylpyridazine
5-Norbornen-2-yl acetate, Mixture of endo and exo
2-Ethoxy-3-Methoxybenzaldehyde
(3R,4R)-1-Benzyl-4-hydroxy-3-pyrrolidinemethanol
4-Bromopentylbenzene
3-(1,3-Dimethylbutylidene)aminopropyl-trithoxysilane
2-Dipropylamino-ethanol
