Hep-3B2.1-7细胞系：人肝癌细胞株 技术硬 货号：GD-C3919019
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
传代细胞培养注意事项：1)掌握好细胞消化的时间，消化时间过短时，细胞不宜从瓶壁脱落，过长的消化会导致细胞脱落、损伤；2)掌握好消化浓度，当消化液浓度过高时，消化时间应缩短，过低时细胞消化时间相对延长。体内细胞培养及其操作步骤：瘤细胞悬液接种1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至10(7)～10(8)/ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位，＞10(6)细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。腹水瘤的建立与腹水瘤的接种：将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下：1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种1×10(6)腹水瘤细胞；3)接种腹水瘤细胞后约7-12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3-5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
传代培养及其操作步骤：原代细胞培养成功以后，需要进行分离培养，否则细胞会因生存空间不足或密度过大，营养障碍，影响细胞生长。细胞由原培养瓶内分离稀释后传到新的培养瓶中培养的过程称之为传代培养。传代细胞允许培养的细胞扩增(形成细胞株)，可以进行细胞克隆，易于保存，但可能丧失一些特殊的细胞和分化特征。传代细胞形成细胞株的利处在于提供了大量持久的实验材料，便于实验。【操作步骤】1)吸掉或倒掉培养瓶内旧培养液；2)向瓶内加入胰蛋白酶液和EDTA混合液少量。以能覆盖培养瓶底为宜；3)置37℃孵箱或室温(25℃温度)下进行消化，2~5min后把培养瓶放在倒置显微镜下进行观察，当发现胞质回缩、细胞间隙增大后，应立即中止消化；4)吸出消化液，向瓶内加入Hanks液少量，轻轻转动培养瓶，把残留消化液冲掉，然后再加培养液。如果仅使用胰蛋白酶消化，在吸除胰蛋白液后，可直接加入少量含血清的培养液，终止消化；5)使用弯头吸管，吸取瓶内培养液，按顺序反复轻轻吹打瓶壁细胞，使之从瓶壁脱离形成细胞悬液。吹打时动作要轻柔，以防用力过猛损伤细胞；6)用计数板计数后，分别接种于新的培养瓶中，置CO2培养箱中进行培养；7)细胞培养换液时间应根据细胞生长的状态和实验要求来确定。一般2～3d后应换一次生长液。待细胞铺满器皿底面，即可使用；也可继续传代扩大培养或换成维持液。【注意事项】1)掌握好细胞消化的时间，消化时间过短时，细胞不宜从瓶壁脱落，过长的消化会导致细胞脱落、损伤；2)掌握好消化浓度，当消化液浓度过高时，消化时间应缩短，过低时细胞消化时间相对延长。
Methyl glycolate, 98%
Ethyl Methyl Carbonate
2-(2-Fluorophenyl)oxazole
(+)-4,5-BIS[HYDROXY(DIPHENYL)METHYL]-2-METHYL-2-PHENYL-1,3-DIOXOLANE
3-CHLORO-2,4-PENTANEDIONE
1-Butaneboronic acid
2-Fluoro-5-(trifluoroMethoxy)benzoic acid, 97%
4-(4-nitrophenoxy)benzoic acid(SALTDATA: FREE)
2-Methyl-6-methoxypyridine-3-boronic acid, 97%
4-(Dicyanomethylene)-2,6-dimethyl-4H-pyran
3-Bromo-4-iodobenzotrifluoride
4-[[(4-Fluorophenyl)imino]methyl]-phenol
2-Amino-3-nitro-6-picoline
2-Amino-3-(1,2-dihydro-2-oxoquinoline-4-yl)propanoic acid
Diallyl phthalate
3-Hydroxy-2-nitropyridine
Diethyl (3-Methoxybenzyl)phosphonate
N-Ethyl 4-broMobenzenesulfonaMide
Trichloroacetyl isocyanate, NMR grade
PotassiuM Carbonate, Sesquihydrate, Crystal, FCC
Heptafluoropropyl Iodide (stabilized with Copper chip)
2,3-Dibromosuccinic acid
2-Amino-5-chloro-3-nitropyridine
Cyclopentanecarbaldehyde
6-Aminonicotinamide
1,4-Dicyanobutane
3-ETHYNYLPYRIDINE
2-(1H-IMidazol-1-YL)EthanaMine Hydrochloride
MethylcyclopentadienylManganese tricarbonyl, 97%
Methyl 1-benzyl-4-oxopiperidine-3-carboxylate hydrochloride, 95%
4-(Methylthio)benzeneboronic acid, 97%
Pyrifenox
2-(1-Methylpropyl)-thiazole
PR(TMHD)3
3-(ChloroMethyl)pentane
4-BroMo-2-fluorotoluene
(3R)-(+)-3-(ETHYLAMINO)PYRROLIDINE
7-Acetoxy-4-methylcoumarin
1H,1H,2H-Perfluorocyclopentane
Ethyl Phenyl Sulfide
5-[4-(tert-Butyl)phenyl]-1H-1,2,4-triazole-3-thiol
ISOCYANOBENZENE
2-FLUORO-DL-PHENYLALANINE
FMOC-SUBEROL
4-Hydroxy-2-(trifluoroMethyl)pyriMidine
18-Crown 6-Ether
4,6-DiMethyl-2-(Methylsulfonyl)pyriMidine
Voriconazole
TryptaMine, 98%
SodiuM Borohydride, Crystalline Powder, Reagent, ACS
5-(4-Chlorobutyl)hydantoin
Ethyl 2-mercaptoacetate
2,2-dimethyl-propanedioic acid dimethyl ester
2-acetylpyrrole
4-Fluoro-2-methoxybenzoic acid
4-PHENOXYBENZONITRILE
2',4',5'-TRIFLUOROPROPIOPHENONE
Bis(diMethylcarbaModithioato-S,S') copper
4-Chloro-5-Methoxy-1-indanone
3-Phenoxypropionic acid, 99%
Magnesium hexafluorosilicate hexahydrate, 96%
Diethyl (4-Isopropylbenzyl)phosphonate
5-Acetyl-quinoline-2,8-diol
2-[4-Aminosulfonyl-phenyl]-ethyl-5-methylpyrazinecarboxamide
N6-BENZYLADENOSINE
1,2,3,4-DIEPOXYBUTANE
Cobalt chloride
2-Chlorostyrene (stabilized with TBC)
1,4-Diethynylbenzene
2-Methyl-4'-(Methylthio)-2-Morpholinopropiophenone
4-(2-Fluoro-4-nitrophenyl)Morpholine
(R)-(+)-Tetrahydro-2-furoic acid, 99% (98% ee)
Acetonylacetone
4-(MethylaMino)benzoic Acid
Trimethylsilyl benzenesulphonate
2-Amino-4-imino-2-thiazoline hydrochloride
allyl cyanoacetate
O-tert-butyl-L-threoninetert-butyl ester acetate
CYTIP ELISA Kit
β2-GP1 Ab lgG ELISA Kit
Human TNS2 ELISA Kit
CD100 ELISA Kit
Ghrelin ELISA Kit
RasGAP ELISA Kit
Chicken IL-13 (Interleukin 13) ELISA Kit
Human corticotropin releasing hormone，CRH ELISA Kit
Human ACE2 ELISA Kit
SNRPD1 ELISA Kit
Thioglycollate Medium (Brewer)
Trichophyton Agar #1
4-Methylvaleryl chloride
AMMoniuM perchlorate, Reagent Grade
5-ChloroMethyl-2-(1H-pyrazol-1-yl)pyridine, 97%
2-Fluorostyrene, 98%, stab. with 0.1% 4-tert-butylcatechol
2,3-Dibromosuccinic acid
ethyl 3,3-diethoxypropionate
Lithium fluoride
N-phthaloyl-L-glutamic acid
3-Acetylphenylboronic acid
ALBUMIN, HUMAN
4-Benzyloxy-L-phenylalanine
Dichloro(ethylenediaMine)palladiuM(II), Pd 44.8%
10-BroMo-1-decanol, 95%
DiMethyl anthranilate
FMoc-Ser-OH
4-Methoxycarbonyl-2-nitrophenylboronic acid
3-HYDROXY-DL-KYNURENINE
2-CHLORO-5-(TRIFLUOROMETHYL)PHENYLACETIC ACID
Methyl 3-aMino-4,4,4-trifluorobutyrate hydrochloride
4-(Trifluoromethyl)-2,3,5,6-tetrafluorothiophenol, 98%
3-Nitrobenzaldehyde
3',3'',5',5''-Tetraiodophenolsulfonphthalein
3-(4-Biphenyl)propionic acid
