Hep 3B(Hep 3B2.1-7)细胞系：人肝癌细胞株 售后强 货号：GD-C3820305
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
复苏细胞注意事项：1)收到细胞后当天换液，全部更换成客户自己的新鲜培养基，放进培养箱，第二天再消化；2)边观察边消化，根据细胞的形态，终止消化时间，采取以半分钟间隔吹打细胞边缘，如可吹打下来，即终止消化。客户摸索比较好的消化时间；3)随细胞有一份细胞操作说明书，请客户仔细查看；4)记得加1%双抗，降低被污染的概率。另外尽早冻存留种，以备后用；5)售后时间为一周，于细胞本身的质量问题，而因乙方自己不当操作(不规范操作，消化过度，不加双抗导致的污染等)导致的细胞问题不在售后范畴；6)收到细胞后，如细胞状态不佳，或培养中遇到问题，烦请当天尽快联系，以便处理。当天及时反馈细胞情况和细胞照片是售后跟踪处理重要的参考依据，请您务必重视；7)刚收到细胞时，胎牛血清可以用15%培养两三天，利于细胞尽快恢复状态，细胞状态稳定后，再调回10%即可(其中1,2条针对于贴壁细胞，悬浮细胞详情请见细胞操作说明书)
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
N-Boc-DL-pipecolinic acid, 98%
SodiuM persulfate
N-CARBOBENZOXY-1,5-DIAMINOPENTANE HYDROCHLORIDE
5-BroMo-2-ethoxypyriMidine
1,3-DIIODOBENZENE
2-(ISOBUTYLTHIO)ETHANOL
2-Deoxy-D-ribose
3-Fluoro-2-methoxy-5-(methylthio)benzeneboronic acid
8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt
(S)-(-)-[(S)-2-Diphenylphosphinoferrocenyl] [2-bis(3,5-dimethyl-4-methoxyphenyl)phosphinophenyl]methanol, min. 97%
5-(2-Pyridinyl)-2-thiophenecarbonyl chloride, 97%
Zirconium carbonate oxide
Allethrin
4-Bromoaniline
α,α′-DibroMo-o-xylene
Methyl 5-Methylsulphonylindole-2-carboxylate
4,6-DiaMinopyriMidine
VariaMine Blue B DiazoniuM Salt [for Naphthol-Dyes]
Tris(diMethylaMino)borane, 98%
Silver Sulfadiazine, Micronized, USP
Benzoic Anhydride
4-Bromobenzonitrile
3-Amino-3-(4-hydroxyphenyl)propanoic acid
N-sodiohexamethyldisilazane
5-Chloro-2-fluorobenzoic acid
PRASEODYMIUM BORIDE
2-Adamantanol
Phenoxyacetic anhydride
4-n-Propoxybenzeneboronic acid, 97%
(R)-1-PhenylbutylaMine, ChiPros|r, 98%, ee 98+%
Boc-Tyr-OH
Orbencarb
2-Fluoro-5-iodotoluene
Phenyl salicylate
Isobutylene oxide
CYCLOHEXENE SULFIDE
Cyclooctylamine
2-Chloro-2-fluoropropane
2-Amino-3,5,6-trifluoroterephthalonitrile
4-(3-Nitrobenzyl), N1-BOC protected 97%
5-ETHYLIDENE-2-NORBORNENE
PALLADIUM(II) OXIDE
4,5-DIMETHYLTHIOPHENE-2-CARBOXYLIC ACID
2,3-DIMETHYL-2-CYCLOPENTEN-1-ONE
Copper(II) nitrate trihydrate
1-Phenyl-5-pyrazolone-3-carboxylic Acid
TetrabutylaMMoniuM Fluoride Hydrate [for Catalyst of acylation, silylation and cleavage of silyl ether]
2,3-Dichlorophenylacetic acid, 97%
1,3-Benzothiazole-6-carboxaldehyde, 97%
7-Dehydrocholesterol
3-Nitroaniline Hydrochloride
Beraprost Sodium
pth-4-hydroxyproline
N,N-dimethylformamide di-tert-butyl acetal
Silicon phthalocyanine dichloride
7-Azaindole
Serinol hydrochloride
2-BroMo-6-butoxypyridine
(S)-Boc-4-Methyl-β-Phe-OH
2,6-Dichloro-4-(trifluoromethyl)benzenesulphonyl chloride 95%
2-Methyl-4-nitro-1H-indole
3-(5-Methyl-tetrazol-1-yl)-phenylamine
(R)-4-Bromostyrene oxide
2,3-Dichloropropene
6-Methoxy-2-naphthol
(R)-4-PHENYL-1,3-THIAZOLIDINE-2-THIONE
N,N-DiethylforMaMide;ForMyldiethylaMine
Myricetin
1-AMinocyclopropanecarboxylic acid hydrochloride
3-(4-Hydroxy-3-Methoxyphenyl)propionic Acid
1-(3,5-Dichlorophenyl)-2,2,2-trifluoroethanone
1-(Phenylsulfonyl)pyrrole, 97%
Myristic acid
TPPS Hydrate (=Tetraphenylporphyrin Tetrasulfonic Acid Hydrate) [Ultra-high sensitive spectrophotoMetric reagent for transition Metals]
clonidine
[1,5]-NAPHTHYRIDIN-2-YLAMINE
4-Biphenylboronic acid
2-Methyl-1-butanethiol
MT ELISA Kit
VGF ELISA Kit
APOC4 ELISA Kit
Human DEFβ118 ELISA Kit
ALV-AG ELISA Kit
JUN ELISA Kit
CCKAR ELISA Kit
Human CAPZβ ELISA Kit
Human angiopoietin-like protein 3,ANGPTL3 ELISA Kit
CH50 ELISA Kit
Modified Mycoplasma Broth Medium Base（Frey）
Sensitivity Disk Broth
IndiuM(III) isopropoxide, 99+% (Metals basis), 5% w/v isopropanol
Ketotifen fumarate
5-Chloro-2(3H)-benzoxazolone, 99%
2-Fluoro-5-Nitropyridine
4-Bromobenzonitrile
Vat Black 38
Tergitol NP-33
Alpha,Alpha,Alpha-Trifluoro-m-toluic acid
4-Methylanisole
Ethopabate
3,4,5-Trifluorobenzaldehyde
N-(4-Pyridyl)thiourea, 98%
2,5-Dichlorothiophene-3-sulfonyl chloride, 97%
Tetrahydrothiophene
Deiquat Monohydrate
(ETHYLTHIO)ACETIC ACID
2-Fluorobiphenyl-3-carboxylic acid
(1,5-Cyclooctadiene)bis(triMethylsilylMethyl)palladiuM(II)
4-BroMo-3-nitrobenzonitrile
2-Methylbutyl Acetate
4-[2-(2-methyl-1H-imidazol-1-yl)ethyl]piperidine(SALTDATA: FREE)
1H-Pyrrolo[2,3-b]pyridin-5-ylamine
Orcein, certified, pure
