MPC-11细胞系：小鼠浆细胞株货期短 货号：GD-C3619041
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
传代细胞培养注意事项：1)掌握好细胞消化的时间，消化时间过短时，细胞不宜从瓶壁脱落，过长的消化会导致细胞脱落、损伤；2)掌握好消化浓度，当消化液浓度过高时，消化时间应缩短，过低时细胞消化时间相对延长。体内细胞培养及其操作步骤：瘤细胞悬液接种1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至10(7)～10(8)/ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位，＞10(6)细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。腹水瘤的建立与腹水瘤的接种：将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下：1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种1×10(6)腹水瘤细胞；3)接种腹水瘤细胞后约7-12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3-5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
1-BroMo-4-(n-octyloxy)benzene, 98%
Dextrin
Methyl 3-(benzyloxy)-4-broMobenzoate
N-Alpha-t-Boc-D-aspartic acid beta-benzyl ester
2-Chloro-3-Methoxybenzonitrile
3-AMinocarbonylphenylboronic acid, pinacol ester
TERT-BUTYL ISOTHIOCYANATE
3,3-Dimethoxy-2-butanone, 98%
1,2-Dichlorobenzene D4 >99%
2,3-Dimethyl-1-pentene
4-Nitrophenol Sodium Salt Dihydrate
5,5-Dimethyloxazolidine-2,4-dione
1-ETHYL-7-CHLORO-6-FLUORO-1,4-DIHYDRO-4-OXOQUINOLINE-3-CARBOXYLIC ACID
1,3-DIISOPROPENYLBENZENE
Butvar B-98
L(-)-Glucose
DodecyltriMethoxysilane
Ethyl 4-broMo-3-Methylbenzoate
Triethylene glycol, 99%
PotassiuM Pyrophosphate, Powder, FCC
Bis(tri-tert-butylphosphine)platinuM(0)
2-Chlorobenzamide
2,3-Dimethoxybenzonitrile
ethyl 2-methyl-1H-pyrrole-3-carboxylate
methyl trifluoromethanesulphonate
CROCONIC ACID
COBALT(II) PHOSPHATE OCTAHYDRATE
Zinc Stearate
Fluphenazine dihydrochloride, 98%
1,4-Bis(4-pyridylMethyl)benzene, 97%
Cyhalofop-butyl
Fluazuron
1-Isocyanato-4-Methoxy-2-nitrobenzene
PotassiuM cyanate
N-UNDECYL ETHER
2-Acetylpyridine
4-Amino-3-methylbenzoic acid
6-Chloro-7-deazapurine ,98%
2,6-Dichloropyrimidine-4-carboxylic acid
Phenethyl-hydrazine
2-Bromo-2-methylpropionic acid
4-Methyl-benzoic acid tert-butyl ester
3-FLUORO-2-HYDROXYPYRIDINE
HexaMethylenetetraMine;Urotropine;Accelerator H;AMinoforM;AMMoforM;MethenaMine;CystaMin;Cystogen;1,3,5,7-TetraazaadaMantane
2-BroMo-5-chloro-3-Methylbenzo[b]thiophene
Benzofuran-3(2H)-one
N,N'-MethylenebisforMaMide
N-Methyl-4-(MethylaMino)picolinaMide
2-Acetylbutyrolactone, 99+%
H-D-LYS(Z)-OH
L-(+)-Gulonic Acid gaMMa-Lactone
gastrin I
BOC-4-HYDROXY-D-PHENYLGLYCINE
Phosphorouspentabromide
2-Octanol
2,3-HEXANEDIONE
4,5-DibroMo-1-trityl-1H-iMidazole
BenzyldodecyldiMethylaMMoniuM broMide
2-TrifluoroMethyl-5-broMopyridine
Boc-gamma-Abu-OH
2,4,5-Trichlorophenyl isothiocyanate
Boc-DL-cyclopentylglycine
1-(1-methyl-1H-benzimidazol-2-yl)ethanone(SALTDATA: FREE)
4,4'-Dichlorobenzophenone
2,6-Naphthalenediol diacetate
YTTRIUM NITRATE HEXAHYDRATE
Orbifloxacin
1-Phenylcyclobutanecarbonitrile
1,5-Diphenyl-3-(p-tolyl)forMazan
4-BroMoacetyl-3-fluorophenylboronic acid
5-BroMo-2-Methylaniline, 98%
2,4-Difluorobenzene-1-carbothioaMide, 97%
SODIUM XYLENESULFONATE
1-CyanoadaMantane
Triphenylmethane
(R)-2-N-Boc-aminomethylmorpholine
boc-L-leucinol
3-Iodobenzoic acid ethyl ester
CYFRA21.1 ELISA Kit
α-SMA ELISA Kit
Human MUC21 ELISA Kit
Human Chlamydia pneumoniae antibody,Cpn-Ab ELISA Kit
PGC-1α ELISA Kit
S-100 ELISA Kit
Chicken Interleukin 2,IL-2 ELISA Kit
Human growth hormone releasing hormone，GHRH ELISA Kit
VEGF-A ELISA Kit
STAT3 ELISA Kit
Fastidious Anaerobe Broth (FAB)
Yeast Synthetic Drop-out Agar Medium without Uraci
IsobutyraMide, 99%
N,N'-DIMETHYLTHIOUREA
5-Chlorobenzo[b]thiophene-3-acetonitrile, 97%
2-Fluoroanisole
2-Chlorobenzamide
Bambermycin
Calcium Iodate
2,6-Difluorobenzoic acid
N-PENTYL-4-AMINOBENZOATE
2-THIOPHENECARBOXYLIC ACID SODIUM SALT
SILVER TRIFLUOROMETHANESULFONATE
3-Fluoro-2-Methylbenzonitrile, 97%
6-Nitroquinoline, 98+%
2,6-Difluorobenzyl alcohol
1-MethylpyridiniuM Chloride
(-)-1,4-DI-O-TOSYL-2,3-O-ISOPROPYLIDENETHREITOL
5-BROMO-2-CHLOROANISOLE
Diethyl azodicarboxylate
1-OCTADECYL-4-(4-PHENYL-1,3-BUTADIENYL)PYRIDINIUM BROMIDE
2-(1H-Benzo[d]imidazol-2-yl)aniline , 97%
3,4,6-Tri-O-acetyl-2-deoxy-D-galactopyranose
3,3-Difluoropyrrolidine hydrochloride
1-Acetamido-4-bromonaphthalene 98%
