Detroit 562细胞株 货号：GD-C1819632

Detroit 562细胞株 货号：GD-C1819632
产品货号：GD-C1819632
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
5-Sulfosalicylic acid dihydrate
Ala-Tyr
Calix[8]arene, 97%
4-(3-Chlorobenzyloxy)-3,5-diMethylphenylboronic acid
6-ChlorochroMone-3-propionic acid
3,3′-DiMethoxybiphenyl
3-(Trifluoromethoxy)benzoic acid
16-Hydroxyhexadecanoic acid, 98%
3-Amino-4-methylbenzyl alcohol
2-(Trifluoromethyl)nicotinonitrile
COBALT (II) PERCHLORATE
4-Chloro-2-(trifluoromethyl)benzonitrile
METHYL (S)-(+)-3-HYDROXY-2-METHYLPROPIONATE
(S)-(-)-2-Azetidinecarboxylic acid
2-HydroxybenziMidazole
3-Hexylthiophene
Ethyl Isopropyl Ether
ChloriMuron-ethyl
1-BroMo-3-phenylpropane, 98%
BROMODICHLOROMETHANE
4-(4-Hydroxyphenyl)benzoic Acid
5-Iodocytosine
Potassium Hypophosphite
trimethyl[(1-methylvinyl)oxy]silane
2-Nitrofluorene
4-Hydroxyphthalic acid
4-Methoxybenzyl ForMate
2-Pentanone, 99+%
4-Benzyloxypyridine N-oxide, 98%
N^a-Boc-N^e-benzyloxycarbonyl-D-lysine, 98%
Hippuric acid
(S)-(+)-3,3-DiMethyl-2-butylaMine
3-Chloro-2,4-difluorobenzylaMine, 97%
N,N-DIMETHYLHEXYLAMINE
4-BroMo-1-(pyridin-2-yl)Methylpyrazole
BOC-4-OXO-L-PROLINE TERT-BUTYL ESTER
2,2-Dibromo-2-cyanoacetamide, 96%
2-Methylthiopyrimidine ,97%
5-Hydroxy-L-tryptophan
5-(Trifluoromethyl)dibenzothiophenium trifluoromethanesulfonate, 96%
N-BOC-HEXAHYDRO-1H-AZEPIN-4-ONE
2-Benzhydrylpiperidine
2-Adamantanamine hydrochloride
Ionone
3-(4-Methoxyphenoxy)benzaldehyde
11-BroMo-1-undecene
3-Ethoxy-5-(Methoxycarbonyl)phenylboronic acid, pinacol ester
DibroModifluoroMethane, 97%
4-BroMo-3,5-diMethylpyrazole, 99%
N-ForMylglycine
LoMerizine Dihydrochloride
N-Carbobenzyloxyglycine
11-Acetoxyundecyltriethoxysilane
N2-Phenylacetyl guanosine
Tetrahydropyran-4-yl-acetic acid
IRON (II) METHOXIDE
(S)-N,N-DIMETHYL-1-[(R)-2-(DIPHENYLPHOSPHINO)FERROCENYL]ETHYLAMINE
ANTIMONY PENTASULFIDE
PotassiuM ethylxanthate
2-(Benzyloxy)ethanamine hydrochloride
5-(Aminosulfonyl)-1-methyl-1H-pyrrole-2-carboxylic acid , 97%
3-Hexyne-2,5-diol
Nonyl Acetate
Chlorpromazine hydrochloride
AMMONIUM MOLYBDATE
CINNAMAMIDE
ALL CIS-7,10,13,16,19-DOCOSAPENTAENOIC ACID ETHYL ESTER
1-(2-Pyridylazo)-2-naphthol
2,2'-Azobis(2,4-diMethylvaleronitrile)
Pht-ala-oh
3-Carboxyphenylboronic acid, 98%
Allyldibutyltin chloride, 97%
Cytarabine
Isophthalaldehyde
4-Nitro-o-xylene
Prohexadione calcium
(5-Methylpyrazin-2-yl)methanol
N,N-dimethyl-1-(2-methyl-1H-indol-3-yl)methanamine
FVII ELISA Kit
Mouse factor B,BF ELISA Kit
GC ELISA Kit
Human ETFDH ELISA Kit
ATF1 ELISA Kit
IL5 ELISA Kit
IL-6 ELISA Kit
Human Hepatitis D virus IgG,HDV IgG ELISA Kit
Human NO ELISA Kit
Mouse 8-iso prostaglandin,8-iso-PG ELISA Kit
Cellulose Decomposing Microorganisms Medium
Agrobacterium rhizogene Medium
Ethyl 3-broMoiMidazo[1,2-a]pyridine-6-carboxylate, 95%
trans-4-HydroxycinnaMic acid, 98%
4-AcetaMido-TEMPO, free radical, 98+%
2-AMinoiophthalic acid
5-Iodocytosine
ditetradecyl 3,3'-thiodipropionate
Nickelous Nitrate
4-Nitrobenzamide

trans,trans-2,4-Decadien-1-al
Sodium 3-sulfobenzoate
Oxalacetic acid, 97%
Benzophenone diMethyl acetal, 98+%
Bis(diethylaMino)chlorophosphine, 94%
2,7-Dihydroxynaphthalene
Octaethylene Glycol Monododecyl Ether
MagnesiuM stearate
3-Methoxyacrylonitrile
1,1-DIBROMOPINACOLONE
AMINOGUANIDINE HYDROCHLORIDE
2-Ethoxy-5-nitropyridine
4'5'-Dimethyl-2'-hydroxyacetophenone
2-(1,1,2,2-Tetrafluoroethoxy)aniline 98%
Boc-(R)-3-amino-4-(4-cyanophenyl)-butyric acid