A7r5大鼠胸大动脉平滑肌细胞株(货号：GD-C0219688)

A7r5大鼠胸大动脉平滑肌细胞株
产品货号：GD-C0219688
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
【细胞培养中细菌、霉菌的污染情况总结】细菌：细菌在普通倒置显微镜下为黑色细沙状，根据感染细菌的不同，可有不同的外形，培养液一般会浑浊变黄，对细胞生长影响明显。仔细检查一下器皿的灭菌情况，是否在高压灭菌时放气时间足够，压力足够！尤其是和储存培养液接触的移液管等物品，连续两次污染的话有可能造成储存液污染，一定要注意！下次使用前检查一下培养液是否存在浑浊的现象！可在培养液中加相应的抗生素处理；霉菌：培养液是清亮的，倒置显微镜下无杂质，37度孵箱培养2-3天，仍清亮，但出现絮状杂质，镜下可见呈细丝状的团状漂浮物，可看到明显的菌丝，细胞仍可生长，但时间长之后，细胞的活力状态变差，用硫酸铜溶液擦拭CO2孵箱内，再把水盘里也加上饱和量的硫酸铜。或者在培养箱的托盘加入饱和的消毒磷酸氢二钠高盐液体,可以防止霉菌污染。CO2孵箱被霉菌污染后，可把所有细胞暂时转移，采用过氧乙酸擦洗孵箱(包括隔板，箱壁)。并把过氧乙酸放置在孵箱内一个小时，使其蒸汽弥漫。待过氧乙酸的气味消散后，再移入细胞。孵箱应定期清洁(2月左右)，尤其在多雨的季节。其它培养箱清洗方法是：用84液擦洗－清水擦洗－75%酒精擦洗－紫外灯照。预防霉菌污染，可在培养基里加3u/ml的两性霉素或制霉菌素或放线菌素D或双抗；但细胞一旦污染，很难挽救，制霉菌素或放线菌素D或双抗都于事无补，建议舍弃该污染细胞，将环境彻底消毒，如果所有细胞都污染，可能是系统污染，检查一下培养基和器材，如果只是个别污染，可能是操作问题，就要注意操作。
Etridiazole
IMidacloprid
PicraMic Acid (wetted with ca. 25% Water, containing 25g on a dry weight basis), 98%
Z-L-Prolinol
Purine
5-Chloro-2-isopropoxypyridine-3-boronic acid
Ammonium sulfide, 20% solution in water
2-Ethoxythiazole
2,2-Difluoro-1,3-benzodioxole-4-boronic acid
6-Chloropyridine-3,4-dicarboxylic acid
2-BROMO-4-FLUOROCINNAMIC ACID
N-(2-Acetamido)iminodiacetic acid disodium salt
Chloromethyltrimethylsilane
4-ISOPROPYL-3-THIOSEMICARBAZIDE
Tri[3,5-di(trifluoroMethyl)phenyl]phosphine
Poly(propylene glycol)
Triacetoxyethylsilane
5-AcetaMido-2-chlorophenol
(R)-3-Pyrrolidinol hydrochloride, 98%
DIAZINON-O-ANALOG
2-Propylfuran
1-Chloro-2,4-difluorobenzene
Magnesium potassium aspartate
3,4-Dimethoxybenzonitrile
trans-1,4-cyclohexanedicarboxylic acid
(S)-(+)-3-Chloro-1,2-propanediol
3-Bromo-2-fluorobenzoic acid
3-Chloro-2-fluoropyridine-4-boronic acid pinacol ester, 95%
2-BroMo-3',4'-dichloroacetophenone, 98%
VinyltriMethylsilane, 98+%
DHBS
3-BROMOBENZHYDRAZIDE
4-(TrifluoroMethylthio)benzyl alcohol
N,N-Bis(2-hydroxyethyl)-3-chloroaniline , 90%
9,10-Dihydro-9-oxa-10-phosphaphenanthrene 10-oxide
Diglycolic anhydride
1-Methylamino-1-methylthio-2-nitroethylene
1,4-Dibromo-2,5-bis(bromomethyl)benzene
2,3,4,5-Tetrahydro-1H-benzo[e][1,4]diazepine
5-(4-methoxyphenyl)-1,3,4-oxadiazol-2-amine(SALTDATA: FREE)
D-Thyroxine
Heptafluorobutyric acid
Trigonelline hydrochloride
Mordant Red 7
5-BroMo-2-chloropyriMidine
1-BroMo-4-(triMethylsilyl)benzene
4-(N,N-DiMethylaMinocarbonyl)Methylphenylboronic acid, pinacol ester
CyclopropylaMine, 99%
MagnesiuM Methyl carbonate, 0.5M solution in Methanol, AcroSeal
Uridine
1-Methoxy-3-(triMethylsilyloxy)-1,3-butadiene
Dibutyl tin diacetate
((2R,3R,4R)-3-(benzoyloxy)-4-fluoro-4-methyl-5-oxotetrahydrofuran-2-yl)methyl benzoate
1-Methyl-3-(trifluoromethyl)-1H-pyrazole-4-carbaldehyde
N-ethyl-N-(3-sulfopropyl)-M-anisidine*sodium
2,5-Dimethyl pyrazine
O-TOLYLMAGNESIUM BROMIDE
4-BroMo-N-dodecyl-1-hydroxy-2-naphthalenecarboxaMide
3-ETHYL-2-METHYLBENZOTHIAZOLIUM IODIDE
(2,4-Dichloro-phenyl)-acetyl chloride
3-Isopropylbenzoic acid
N,N'-Dihexylthiourea, 97%
5-(4-Hydroxypiperidin-1-yl)thiophene-2-carboxaldehyde
Pent-2-ene-1,5-dioic acid
3-Cyano-4-fluorobenzotrifluoride
2,2,2-Tribromoethanol
SODIUM COBALTINITRITE
LithiuM acetate
2,2-DiMethylpentane
3-Methoxyflavone
FMoc-dap(alloc)-oh
4-ChroManol, 96%
L-ALPHA-PHOSPHATIDYLCHOLINE, DISTEAROYL
Tris(hydroxyMethyl)aMinoMethane Hydrochloride
thiamine nitrate
Pipotiazine
methyl 3-aminocrotonate
Direct Brown 44
ES ELISA Kit
AP13 ELISA Kit
Human IGFBP3 ELISA Kit
Human LDL ELISA Kit
CEP110 ELISA Kit
Rat CD3 (Cluster of Differentiation 3) ELISA Kit
DHT ELISA Kit
Human TNKS1 ELISA Kit
Human IRS-2 ELISA Kit
COL3 ELISA Kit
ISP Medium 7( Tyrosine Agar Medium Base)
Folic Acid Assay Medium
Ethyl 1-(hydroxyMethyl)cyclohexanecarboxylate, 97%
trans-2-Hexenoic acid
4-Ethyl-trans-^b-styrylboronic acid pinacol ester, 97%
Aniline-2-sulfonic acid
1-Chloro-2,4-difluorobenzene
3,5-Dichloro-4-hydroxypyridine
ammonium [R-(R*,R*)]-tartrate
5,5-diphenylhydantoin sodium sigmaultra

1,4-DICHLORO-2-IODOBENZENE
L-LACTIC ACID LITHIUM SALT
Diethyl ChloroMalonate
2-(2-Fluorophenoxy)ethylaMine, 97%
3-BroMo-4-Methoxybenzyl alcohol, 98%
2'-Deoxyguanosine hydrate, 99%
Methyl stearate
3-BroMo-5-butoxyphenylboronic acid
ETHYL 2,5-DICHLOROBENZOATE
2,4,5-Trifluorophenyl isocyanate
Mercaptosuccinic acid
2-Chloro-4,6-bis[3-(perfluorohexyl)propyloxy]-1,3,5-triazine
Propiolic Acid
1-Pentyn-3-ol
3,6-Dibromo-o-xylene