KU812人外周血嗜碱性白细胞株(货号：GD-C0219554)

KU812人外周血嗜碱性白细胞株
产品货号：GD-C0219554
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
[6]-Shogaol
2,7-DibroMo-9-fluorenone
PTH-VALINE
2-(MethylaMino)biphenyl-3-carboxylic acid
TRANS-3-METHYL-2-PENTENE
2-Chloro-1-Methyl-1H-iMidazole
Tri-tert-butylphosphine, 95%
Allyl chloride, stabilized, 98%
1-[3-(2-chlorophenyl)-1,2,4-oxadiazol-5-yl]methanamine(SALTDATA: HCl)
Methyl 4-bromo-3-fluorobenzoate 98%
4-Pyrimidinol
METHYL 3-BENZOYLPROPIONATE
4,6-Dichloro-5-methylpyrimidine
BETA-BUTYROLACTONE
3-Buten-2-ol
4-Chloro-2,5-diMethylaniline
N,N'-EthylenebisoctadecanaMide
4-(4-ForMylphenyl)phenol
N,N-DiforMylacetaMide, 80%
4,4-METHYLENE-BIS-(2-CHLOROANILINE)
4,6-Decadiyne
5-methyl-1H-indole-3-ethylamine monohydrochloride
1-(3,5-anhydro-2-deoxypentofuranosyl)-5-methylpyrimidine-2,4(1H,3H)-dione
3-Chlorobenzhydrazide
3-Hydroxy-2-nitrobenzoic acid
Monosodium fumarate
PODOPHYLLOTOXIN
N-Isopropyl-N-Phenyl-1,4-PhenylenediaMine
1-(2-Thiazolyl)piperazine hydrochloride, 97+%
2-Phenylanthraquinone, 98%
4′-(Octyloxy)-4-biphenylcarbonitrile
Phenylhydrazine sulfate
2-ForMylphenylboronic acid MIDA ester
N-Benzyl-N-Methyl 2-broMobenzaMide
2,3,4,5,6-PENTAFLUOROPHENYLACETONITRILE
Iron hydroxide oxide
D-Alaninol
5-Chloro-3-iodo-1H-indazole
4-Butoxyaniline
2-Bromo-5-methoxy-3-methylbenzoic acid
5-Aminosalicylic acid
2-CHLORO-4-(TRIFLUOROMETHOXY)ANILINE
Betahistine dihydrochloride
Tributylphosphane
Pyridazine
1-AMinotridecane
(2-Methylpyridin-4-yl)Methanol
3-Chlorophenethylalcohol, 98%
2,6-DiMethoxyaniline, 96%
Palladium(II)-ammonium chloride
Methylene Glycol Bis(2,3-dibroMopropyl) Ether
1-isopropyl-4-methylcyclohexane
Fmoc-7-amino-heptanoic acid
1-Aminoindane
Copper(I) trifluoromethanesulfonate benzene complex (2:1)
4-Fluorophenoxyacetic acid
4-Methoxyphenyl 3-O-Allyl-4,6-O-benzylidene-2-deoxy-2-phthaliMido-beta-D-glucopyranoside , 97%
3-(3-Fluoro-4-Methylphenyl)aniline
Isodecyl Methacrylate
Benzyldimethylchlorosilane
1-[3-Chloro-5-(trifluoromethyl)-2-pyridyl]-1,4-diazepane, 97%
n-Nonadecanonitrile
4-Ethylphenylmagnesium bromide, 0.5M solution in THF, AcroSeal
Chloramphenicol sodium succinate
1H,1H,2H,2H-PERFLUORODECANETHIOL
Trifluoromethyl iodide
POLYAMIDE 6 HPLC 0.005-0.020MM (5-20UM)&
Isobutyl ChloroforMate
2-(4-tert-Butylphenyl)-5-(4-biphenylyl)-1,3,4-oxadiazle
2-Methyl-3-hexanone
Methyl 4-hydroxy-7,8-diMethylquinoline-2-carboxylate
2-Naphthyl benzoate, 98%
2-Methylindole
Stearyl Acrylate (stabilized with MEHQ)
Garlic Oil
p-Tolylmethyldichlorosilane
5-Vinylpyrrolidone
5-Methylindolin-2-one
PTGES ELISA Kit
CX3CR1 ELISA Kit
HBsAg ELISA Kit
Human LOR ELISA Kit
Monkey CRP (C-reactive protein) ELISA Kit
IL-22 ELISA Kit
VCAM1 ELISA Kit
Human RETNLβ ELISA Kit
Human elongin A,EloA ELISA Kit
C1orf85 ELISA Kit
N.gonorrhoeae Agar Medium Base
GC Agar Base
Hydantoin-5-acetic acid, 98%
Ethyl trans-2-hexenoate, 98%
4-Ethoxystyrene, 97%, stab. with 0.1% 4-tert-butylcatechol
2-Phenyl-2-propanol, 98+%
5-methyl-1H-indole-3-ethylamine monohydrochloride
Methyldiethoxyphosphine
ethyl (S)-(+)-mandelate
iodipamide

1,2,2,6,6-PENTAMETHYLPIPERIDINE
DIACETOXYDIMETHYLSILANE
N-ButylMorpholine
VanadiuM(IV) oxide, 99% (Metals basis)
2-Chloro-6-Methylpyrazine, 97%
1,3-Dichloro-2-propanol, 98+%
Z-Thr-OH
N-Acetyl 2-fluoro-4-(3-hydroxy-3-Methylbut-1-ynyl)aniline
2-Ethoxy-3-forMyl-5-Methylphenylboronic acid
4-Nitrophenyl forMate
Cyclohexene
4-BroMo-1,2-(Methylenedioxy)benzene
3-Bromo-5-methoxybenzoic acid 99%
2-Methyl-6-nitrobenzoic Anhydride
Methyl 2-(3-fluorophenyl)acetate