MKN74胃癌细胞株
产品货号：GD-C0119279
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
【细胞培养中支原体、黑蛟虫、真菌的污染情况总结】支原体：黑色的，好象多为多形，培养液一般培养液一般会浑浊，原体感染，国内血清很多都没有做支原体阴性检测，而支原体是牛血清中Z常见的微生物之一。而且它不能用过滤的办法除去。支原体感染细胞以后，细胞病变不很明显，只是慢慢死去。用泰乐菌素，兽用支原体病的药，但可用于细胞培养，无任何不良反应。如果作为常用的抗生素的话, 建议用8ug/ml的浓度；黑蛟虫：可以穿透滤膜，也可以通过空气传播，低倍下为黑色点状，高倍下可看见黑色的小虫游来游去，培养液也是不浑的，一般不会太影响，细胞还是可以用的。常常是细胞生长状态良好，且观测到的运动物无明显增多，且培养液颜色、透明度无明显变化，可在同一批号的血清养的细胞中发现类似现象。对细胞生长状态不会有明显影响，在细胞增殖旺盛之后会自然消失，除更换血清外无须特殊处理。建议如果细胞有可能是此种污染的话，可以增加细胞的种板密度，以提高细胞的生存率；真菌：一般培养液清亮，不变色，镜下有丝状物，有些真菌开始很像死细胞碎片，只是它很多很多的小块很清楚，象珊瑚状，不象细胞碎片分不清，慢慢的会长出很细的黑色丝状物。真菌生长的比较慢，不象细菌那么容易被发现，但是一旦发现有它的存在细胞就被污染了，也很难救活了。
Triphenyl(2-pyridylMethyl)phosphoniuM chloride hydrochloride, 98%
6-AMinopyridine-2-carboxylic acid
2-Cyclohexylvinylboronic acid
N-(3-BROMOPROPYL)PHTHALIMIDE
Ethyl 1-naphthaleneacetate
5,6-DIAMINO-2,3-DICYANOPYRAZINE
2-(TRIPHENYLPHOSPHORANYLIDENE)PROPIONALDEHYDE
Methyl 2,5-difluorobenzoate
6-tert-Butyl-m-cresol
Imidazo[1,2-a]pyridine-6-boronic acic pinacol ester
2-[2-(Diphenylphosphino)ethyl]pyridine, min. 97%
2-Boc-aminomethylpyrrolidine
(S)-3-AMINO-4-(2,4-DICHLOROPHENYL)BUTANOIC ACID HYDROCHLORIDE
1-(4-FLUOROPHENYL)-2-THIOUREA
Triphenylene
2-Methyl-1,3-thiazole-4-carbothioaMide
2,5-Dihydro-4-Methyl-2,5-dioxo-3-furanpropanoic Acid
Methyl 2-broMo-5-Methylbenzoate
4-Morpholinophenylboronic acid, 97%, May contain varying aMounts of anhydride
Sulfurous Acid, Reagent, ACS
Ethyl 2,4-Difluorobenzoate
Trimethylhydroquinone
2-amino-6-chloro-4-nitrophenol
2-allyl-1,4-bis[[3-(3,4-dihydroxyphenyl)-1-oxo]oxy]-3,5-dihydroxycyclohexanecarboxylic acid
3,5-Dimethoxyphenol
ISOXAZOLE-5-CARBOXYLIC ACID
3-Hydroxy-4-methoxybenzoic acid
N-Boc-trans-4-Hydroxy-L-proline Methyl ester
1,13-Tetradecadiene, 90+%
(S)-1-Boc-2-isobutylpiperazine, 97%
Tetraethylene glycol diMethacrylate
5-Chloro-2-Methylindole
1,3,5-Tri(9-carbazolyl)benzene
N-Z-O-Benzyl-L-threonine
6-Methoxynicotinonitrile
2,2-DIMETHYLTETRAHYDROPYRAN-4-ONE
2-Bromo-1-(2-thienyl)-1-propanone
3-Bromobenzhydrazide
Chlorophenol Red-β-D-galactopyranoside ,90%
2-(1H-indol-1-yl)ethanamine(SALTDATA: FREE)
7-Bromoheptanenitrile
Pyridine methanesulfonate
2-ETHYL-1H-INDENE
2-Methyl-1-butanol;sec-Butylcarbinol;Active aMyl alcohol
(R)-(-)-1-Methyl-3-phenylpropylaMine
2-AMino-5-chloro-3-Methylpyridine
2,2-Difluoro-2-(4-nitrophenyl)acetic acid
4-BroMobenzotrifluoride, 99%
(-)-DiMenthyl fuMarate, 95%
Trifluridine
Hydrazine L-(+)-Tartrate
Diethyl maleate
2-deoxy-2-phthalimido-3,4,6-tri-O-*acetyl-A-D-
L-Isoleucine tert-butyl ester hydrochloride
4'-methyl[1,1'-biphenyl]-4-carbonitrile
2-Cyanoethyl N,N-diisopropylchlorophosphoramidite
4-Isopropylpiperidine
ETHYL 4-METHYL-4-PENTENOATE
ETHYL METHYL SULFONE
2-Piperazin-1-yl-2-phenylacetic acid, N4-BOC protected
Terbium(III) carbonate hydrate (99.9%-Tb) (REO)
4-(Bromomethyl)-3-fluorobenzonitrile
Bis(triphenylphosphine)iminium chloride, 97%
HOMOGENTISIC ACID GAMMA-LACTONE
4-Ethylacetophenone
Methyl trimethylacetate
Calcium chloride dihydrate
CalciuM sulfate dihydrate
1-Butyl-2,3-diMethyliMidazoliuM tetrafluoroborate
2-Isopropoxy-4,4,6-triMethyl-1,3,2-dioxaborinane
Methyl 2-fluoroacrylate, BHT stablized
IodonitrotetrazoliuM chloride, 98%
2-[(4-Methylphenyl)sulfonyl]ethanol
4-Fluorobenzenesulfonyl Chloride
6-Chloro-1,3-dimethyluracil
2-(4-Bromophenyl)naphthalene
cyclohexyldiphenylphosphine
2-phenoxyaniline
leptin ELISA Kit
Mouse P-Selectin ELISA Kit
Human PCDH15 ELISA Kit
Human DPPⅣ ELISA Kit
NTX ELISA Kit
β-Defensin ELISA Kit
IgY ELISA Kit
Human ATP1B4 ELISA Kit
Human Serum amyloid P，SAP ELISA Kit
HP-CagA-lgA ELISA Kit
MR-VP Medium
Peptone Sorbitol Bile salts Broth
Bis(ethylene)(2,4-pentanedionato)rhodiuM(I), Rh 39.9% Min
PotassiuM fluoride, 99.9% (Metals basis)
Ethyl 5-(tri-n-butylstannyl)isoxazole-3-carboxylate, 96%
2-Furoyl Chloride
Trimethylhydroquinone
5,6,7,8-TETRAHYDRO-[1,7]NAPHTHYRIDINE
Tetraethyl orthosilicate
Suberic acid
2-Fluoro-3-methylpyridine
4,6-Dichloro-5-PyriMidinecarbaldehyde
Fluorolink|rD10-H
N,N-DiMethylacrylaMide, 99.5%, stab. with 100ppM 4-Methoxyphenol
PotassiuM hydrogen sulfate, 97%
H-Dapa-OH·HCl
Pentadecyl isocyanate
(R)-(-)-Mandelic acid
2,4-Dichlorophenylboronic acid, pinacol ester
Adenine
1-(3-Chlorophenyl)pyrazole-4-boronic acid
2-Amino-4-phenyl-6-(trifluoromethyl)pyrimidine 97%
3-Carboxy-5-nitrophenylboronic Acid (contains varying amounts of Anhydride)
Methyl N-butylsulfonyl-L-p-hydroxyphenylalanine
Dimethyldistearylammonium Chloride
