CCD-18Co正常人结肠组织细胞株
产品货号:GD-C719015
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
【悬浮型细胞的传代操作】1)吸出细胞培养液,放入离心管中,离心1000rpm 5分钟;2)吸掉上清液,加入适量之新鲜培养基,混和均匀后,依稀释比例转移至新的培养瓶中,以正常培养条件培养。【注意事项】1)严格的无菌操作;2)适度消化:消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响,消化过程中应该注意培养细胞形态的变化,一旦胞质回缩,连接变松散,或有成片浮起的迹象就要立即终止消化。附:EDTA(0.02%乙二胺四乙酸二钠)消化液配方:EDTA 0.20g,NaCl 8.00g,KCl 0.20g,KH2PO4 0.02g,葡萄糖 2.00g,0.5%酚红4ml,加入蒸馏水定容至1000ml。10磅20min高压灭菌,使用时调节PH值到7.4。注意EDTA不能被血清中和,使用后培养瓶要彻底清洗,否则再培养时细胞容易脱壁。
2-Chloro-N-MethylacetaMide, 97%
HexachloroiridiuM Acid Hydrate
3-BroMo-2-broMoMethyl-1-propene
Acetosyringone
ETHYL 4-CHLORO-6,8-DIMETHYLQUINOLINE-3-CARBOXYLATE
4-AMINO-N,N-DIMETHYLBENZAMIDE
Bis[(triMethylsilyl)tributyl]stannyl phosphate
(4-bromo-3,5-dimethyl-1H-pyrazol-1-yl)acetonitrile(SALTDATA: FREE)
Vinyldiphenylphosphine, min. 97%
2-Methylbenzyl isothiocyanate
4-Cyano-2-iodopyridine 98%
MILTEFOSINE
Bis(1,5-cyclooctadiene)rhodium(I) tetrafluoroborate
N-BENZYL-L-PROLINE
EDCI
TributylaMine
1,3-DibroMo-5-(trifluoroMethoxy)benzene
N-(2,6-DiMethylphenyl) 2-broMobenzaMide
Trichloroacetic acid, 99%, extra pure
PoloxaMer 407, NF
Mordant Blue 13
2,6-Dibromoaniline
L-Lysine L-glutamate
2-Mercaptoimidazole
Methyl 4-fluoro-3-nitrobenzoate
3,5-Dibenzyloxyacetophenone
NYLON 6/66
1,3,3-TriMethyl-2-Methyleneindoline
SodiuM tetrafluoroborate, 97%
Quinoline-4-carboxylic acid, 98+%
SodiuM oxalate
Phenylhydrazine hydrochloride
O-BENZYL-L-SERINE
PRASEODYMIUM(III) IODIDE
2,6-Dichloro-3-Methylpyridine
1-(2-Chlorophenyl)piperazine
4,5-Dibromo-1H-imidazole 98%
d-Leucinol
Disodium beta-Glycerophosphate Tetrahydrate [for Biochemical Research]
3-[(tert-Butoxycarbonyl)amino]-3-(4-methylphenyl)propanoic acid
N,N'-(3,3'-Dimethyl-4,4'-biphenyldiyl)bis(3-oxobutanamide)
4-ETHOXYSTYRENE
5-Phenylhydantoin
5-Methylphenazinium methosulfate
Cobalt tetraMethoxyphenylporphyrin
5-AMino-1-naphthalenesulfonic acid
trans-4-(HydroxyMethyl)cyclohexanecarboxylic Acid
2-Chloro-4-hydroxy-5-fluoropyriMidine
BOC-trans-4-hydroxy-L-proline, 98%
6-PHOSPHOGLUCONIC ACID TRISODIUM SALT
4-Nitrobenzyl Alcohol
4,4'-cyclohexane-1,1-diylbis(2-cyclohexylphenol)
Vinyltriisopropoxysilane
2,3-Dimethoxyphenol
2-allyl-1,4-bis[[3-(3,4-dihydroxyphenyl)-1-oxo]oxy]-3,5-dihydroxycyclohexanecarboxylic acid
(-)-ANABASINE
2-Isopropyl-4-Methylthiazole-5-boronic acid, pinacol ester
2-BroMo-5-chlorophenylboronic acid
CalciuM dipropionate
Glycyl-4-aminobutyric Acid
n-Propyldichlorophosphine, min. 98%
Triphenylantimony Diacetate
1,4-Cyclohexanedimethanol Diisovalerate
N-ACETYLPHTHALIMIDE
(S)-N-FMOC-AZETIDINE-2-CARBOXYLIC ACID
Thifensulfuron methyl
Tungsten
[4-(1,3-Thiazol-2-yl)phenyl]MethylaMine
1,4-DibroMonaphthalene
Methyl trans-4-Hydroxycyclohexanecarboxylate
5-Fluoro-2-Methyl-4-nitroaniline
BroMoforM, 96%, stabilized
MYRISTOLEIC ACID
Chlorogenic Acid Hydrate
DYNASYLAN 9407
3-Pyridinecarboxaldehyde, 2-Fluoro-
Ehoxyphenylpiperazine; 97%
3,7-Diazabicyclo[3.3.0]octane
FLNa ELISA Kit
β2-GP1 Ab lgA ELISA Kit
Human LATS ELISA Kit
Human SNX10 ELISA Kit
MYO/MB ELISA Kit
SP-Selectin/GMP-140/CD62P ELISA Kit
Chicken IL-12 p40 (Interleukin 12 p40) ELISA Kit
Human FSH ELISA Kit
Human Angiotensin converting enzyme,ACE ELISA Kit
ADA ELISA Kit
Clostridia Count Agar
Yeast Peptone Dextrose Adenine Medium
Methyl isocyanoacetate, 95%
DysprosiuM(III) perchlorate hexahydrate, 50% w/w aq. soln., Reagent Grade
5-Chloroquinaldine
2-Fluoropyridine-3-boronic acid pinacol ester, 95%
2,6-Dibromoaniline
1-(5-methyl-2-furyl)propan-1-one
Copper(II) fluoride
9a-fluoroprednisolone
2-Indanylacetic acid
n-Undecane
RhodiuM(III) sulfate, bright plating solution, Rh 5% (cont. Rh)
1-Boc-3-iodoazetidine, 97%
Iron powder, reduced, 99%
PhenylMethanesulfonyl fluoride
RhodiuM acetate
Boc-Phe-ONp
4-(TRIFLUOROMETHYL)PHENYL ISOCYANATE
NeopentylaMine
3-ETHOXYBENZHYDRAZIDE
Manganese(IV) oxide, powder, 80-85%
5-Phenyl-1,3-oxazole, 95%
Ethyl pentafluoropropionylacetate
Bis(tricyclohexylphosphine)[(phenylthio)methylene]ruthenium(II) dichloride, min. 97%