Detroit 562人咽头癌胸水转移细胞株(货号：GD-C619632)

Detroit 562人咽头癌胸水转移细胞株
产品货号：GD-C619632
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
很多朋友讨论细胞培养问题，见到很多很好的经验总结，这里说一些我个人的经验，因为一些比较特殊的原因，我自己培养接触过近300种细胞，常用于做实验的，累积有百余种，可以说遇到过许多各式各样古怪的细胞。这里挑细胞消化开始做我的篇ZT，并不是因为细胞消化Z重要，而是近期看到大家频繁讨论这个话题，我就抛砖引玉，下面几点拙见，可能与其它朋友总结的一些经验有点出入，仅供大家参考。许多同学对细胞消化做了许多研究，得出了很多有价值的经验，也见到很多人的困惑。其实细胞培养，尤其是细胞系的培养，就消化而言，不是太难，做得多了，善于总结经验，就能把细胞越养越好。一般的程序步骤，细节操作，我这里就不讲了，只讲一些基本操作背后的知识，如果不对之处，欢迎指正，总结下来，细胞培养中消化很重要，但其实消化并不是细胞培养的关键所在(虽然很重要)，关键所在是细胞来源，血清质量和水源(自己配制溶液的话)。我看到版上许多人养细胞遇到各种各样的问题，很多时候bottleneck没有找到，虽然通过其它方法有所改善，但仍然是事倍功半。因为人们往往注意自己的操作，总觉得自己没有经验，而忽视试剂，溶液尤其是细胞的质量，后者其实才是许多细胞培养实验室常见的问题。
5-Sulfosalicylic acid dihydrate
Ala-Tyr
Calix[8]arene, 97%
4-(3-Chlorobenzyloxy)-3,5-diMethylphenylboronic acid
6-ChlorochroMone-3-propionic acid
3,3′-DiMethoxybiphenyl
3-(Trifluoromethoxy)benzoic acid
16-Hydroxyhexadecanoic acid, 98%
3-Amino-4-methylbenzyl alcohol
2-(Trifluoromethyl)nicotinonitrile
COBALT (II) PERCHLORATE
4-Chloro-2-(trifluoromethyl)benzonitrile
METHYL (S)-(+)-3-HYDROXY-2-METHYLPROPIONATE
(S)-(-)-2-Azetidinecarboxylic acid
2-HydroxybenziMidazole
3-Hexylthiophene
Ethyl Isopropyl Ether
ChloriMuron-ethyl
1-BroMo-3-phenylpropane, 98%
BROMODICHLOROMETHANE
4-(4-Hydroxyphenyl)benzoic Acid
5-Iodocytosine
Potassium Hypophosphite
trimethyl[(1-methylvinyl)oxy]silane
2-Nitrofluorene
4-Hydroxyphthalic acid
4-Methoxybenzyl ForMate
2-Pentanone, 99+%
4-Benzyloxypyridine N-oxide, 98%
N^a-Boc-N^e-benzyloxycarbonyl-D-lysine, 98%
Hippuric acid
(S)-(+)-3,3-DiMethyl-2-butylaMine
3-Chloro-2,4-difluorobenzylaMine, 97%
N,N-DIMETHYLHEXYLAMINE
4-BroMo-1-(pyridin-2-yl)Methylpyrazole
BOC-4-OXO-L-PROLINE TERT-BUTYL ESTER
2,2-Dibromo-2-cyanoacetamide, 96%
2-Methylthiopyrimidine ,97%
5-Hydroxy-L-tryptophan
5-(Trifluoromethyl)dibenzothiophenium trifluoromethanesulfonate, 96%
N-BOC-HEXAHYDRO-1H-AZEPIN-4-ONE
2-Benzhydrylpiperidine
2-Adamantanamine hydrochloride
Ionone
3-(4-Methoxyphenoxy)benzaldehyde
11-BroMo-1-undecene
3-Ethoxy-5-(Methoxycarbonyl)phenylboronic acid, pinacol ester
DibroModifluoroMethane, 97%
4-BroMo-3,5-diMethylpyrazole, 99%
N-ForMylglycine
LoMerizine Dihydrochloride
N-Carbobenzyloxyglycine
11-Acetoxyundecyltriethoxysilane
N2-Phenylacetyl guanosine
Tetrahydropyran-4-yl-acetic acid
IRON (II) METHOXIDE
(S)-N,N-DIMETHYL-1-[(R)-2-(DIPHENYLPHOSPHINO)FERROCENYL]ETHYLAMINE
ANTIMONY PENTASULFIDE
PotassiuM ethylxanthate
2-(Benzyloxy)ethanamine hydrochloride
5-(Aminosulfonyl)-1-methyl-1H-pyrrole-2-carboxylic acid , 97%
3-Hexyne-2,5-diol
Nonyl Acetate
Chlorpromazine hydrochloride
AMMONIUM MOLYBDATE
CINNAMAMIDE
ALL CIS-7,10,13,16,19-DOCOSAPENTAENOIC ACID ETHYL ESTER
1-(2-Pyridylazo)-2-naphthol
2,2'-Azobis(2,4-diMethylvaleronitrile)
Pht-ala-oh
3-Carboxyphenylboronic acid, 98%
Allyldibutyltin chloride, 97%
Cytarabine
Isophthalaldehyde
4-Nitro-o-xylene
Prohexadione calcium
(5-Methylpyrazin-2-yl)methanol
N,N-dimethyl-1-(2-methyl-1H-indol-3-yl)methanamine
FVII ELISA Kit
Mouse factor B,BF ELISA Kit
GC ELISA Kit
Human ETFDH ELISA Kit
ATF1 ELISA Kit
IL5 ELISA Kit
IL-6 ELISA Kit
Human Hepatitis D virus IgG,HDV IgG ELISA Kit
Human NO ELISA Kit
Mouse 8-iso prostaglandin,8-iso-PG ELISA Kit
Cellulose Decomposing Microorganisms Medium
Agrobacterium rhizogene Medium
Ethyl 3-broMoiMidazo[1,2-a]pyridine-6-carboxylate, 95%
trans-4-HydroxycinnaMic acid, 98%
4-AcetaMido-TEMPO, free radical, 98+%
2-AMinoiophthalic acid
5-Iodocytosine
ditetradecyl 3,3'-thiodipropionate
Nickelous Nitrate
4-Nitrobenzamide

trans,trans-2,4-Decadien-1-al
Sodium 3-sulfobenzoate
Oxalacetic acid, 97%
Benzophenone diMethyl acetal, 98+%
Bis(diethylaMino)chlorophosphine, 94%
2,7-Dihydroxynaphthalene
Octaethylene Glycol Monododecyl Ether
MagnesiuM stearate
3-Methoxyacrylonitrile
1,1-DIBROMOPINACOLONE
AMINOGUANIDINE HYDROCHLORIDE
2-Ethoxy-5-nitropyridine
4'5'-Dimethyl-2'-hydroxyacetophenone
2-(1,1,2,2-Tetrafluoroethoxy)aniline 98%
Boc-(R)-3-amino-4-(4-cyanophenyl)-butyric acid