KU812人外周血嗜碱性白细胞株(货号：GD-C519554)

KU812人外周血嗜碱性白细胞株
产品货号：GD-C519554
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
很多朋友讨论细胞培养问题，见到很多很好的经验总结，这里说一些我个人的经验，因为一些比较特殊的原因，我自己培养接触过近300种细胞，常用于做实验的，累积有百余种，可以说遇到过许多各式各样古怪的细胞。这里挑细胞消化开始做我的篇ZT，并不是因为细胞消化Z重要，而是近期看到大家频繁讨论这个话题，我就抛砖引玉，下面几点拙见，可能与其它朋友总结的一些经验有点出入，仅供大家参考。许多同学对细胞消化做了许多研究，得出了很多有价值的经验，也见到很多人的困惑。其实细胞培养，尤其是细胞系的培养，就消化而言，不是太难，做得多了，善于总结经验，就能把细胞越养越好。一般的程序步骤，细节操作，我这里就不讲了，只讲一些基本操作背后的知识，如果不对之处，欢迎指正，总结下来，细胞培养中消化很重要，但其实消化并不是细胞培养的关键所在(虽然很重要)，关键所在是细胞来源，血清质量和水源(自己配制溶液的话)。我看到版上许多人养细胞遇到各种各样的问题，很多时候bottleneck没有找到，虽然通过其它方法有所改善，但仍然是事倍功半。因为人们往往注意自己的操作，总觉得自己没有经验，而忽视试剂，溶液尤其是细胞的质量，后者其实才是许多细胞培养实验室常见的问题。
[6]-Shogaol
2,7-DibroMo-9-fluorenone
PTH-VALINE
2-(MethylaMino)biphenyl-3-carboxylic acid
TRANS-3-METHYL-2-PENTENE
2-Chloro-1-Methyl-1H-iMidazole
Tri-tert-butylphosphine, 95%
Allyl chloride, stabilized, 98%
1-[3-(2-chlorophenyl)-1,2,4-oxadiazol-5-yl]methanamine(SALTDATA: HCl)
Methyl 4-bromo-3-fluorobenzoate 98%
4-Pyrimidinol
METHYL 3-BENZOYLPROPIONATE
4,6-Dichloro-5-methylpyrimidine
BETA-BUTYROLACTONE
3-Buten-2-ol
4-Chloro-2,5-diMethylaniline
N,N'-EthylenebisoctadecanaMide
4-(4-ForMylphenyl)phenol
N,N-DiforMylacetaMide, 80%
4,4-METHYLENE-BIS-(2-CHLOROANILINE)
4,6-Decadiyne
5-methyl-1H-indole-3-ethylamine monohydrochloride
1-(3,5-anhydro-2-deoxypentofuranosyl)-5-methylpyrimidine-2,4(1H,3H)-dione
3-Chlorobenzhydrazide
3-Hydroxy-2-nitrobenzoic acid
Monosodium fumarate
PODOPHYLLOTOXIN
N-Isopropyl-N-Phenyl-1,4-PhenylenediaMine
1-(2-Thiazolyl)piperazine hydrochloride, 97+%
2-Phenylanthraquinone, 98%
4′-(Octyloxy)-4-biphenylcarbonitrile
Phenylhydrazine sulfate
2-ForMylphenylboronic acid MIDA ester
N-Benzyl-N-Methyl 2-broMobenzaMide
2,3,4,5,6-PENTAFLUOROPHENYLACETONITRILE
Iron hydroxide oxide
D-Alaninol
5-Chloro-3-iodo-1H-indazole
4-Butoxyaniline
2-Bromo-5-methoxy-3-methylbenzoic acid
5-Aminosalicylic acid
2-CHLORO-4-(TRIFLUOROMETHOXY)ANILINE
Betahistine dihydrochloride
Tributylphosphane
Pyridazine
1-AMinotridecane
(2-Methylpyridin-4-yl)Methanol
3-Chlorophenethylalcohol, 98%
2,6-DiMethoxyaniline, 96%
Palladium(II)-ammonium chloride
Methylene Glycol Bis(2,3-dibroMopropyl) Ether
1-isopropyl-4-methylcyclohexane
Fmoc-7-amino-heptanoic acid
1-Aminoindane
Copper(I) trifluoromethanesulfonate benzene complex (2:1)
4-Fluorophenoxyacetic acid
4-Methoxyphenyl 3-O-Allyl-4,6-O-benzylidene-2-deoxy-2-phthaliMido-beta-D-glucopyranoside , 97%
3-(3-Fluoro-4-Methylphenyl)aniline
Isodecyl Methacrylate
Benzyldimethylchlorosilane
1-[3-Chloro-5-(trifluoromethyl)-2-pyridyl]-1,4-diazepane, 97%
n-Nonadecanonitrile
4-Ethylphenylmagnesium bromide, 0.5M solution in THF, AcroSeal
Chloramphenicol sodium succinate
1H,1H,2H,2H-PERFLUORODECANETHIOL
Trifluoromethyl iodide
POLYAMIDE 6 HPLC 0.005-0.020MM (5-20UM)&
Isobutyl ChloroforMate
2-(4-tert-Butylphenyl)-5-(4-biphenylyl)-1,3,4-oxadiazle
2-Methyl-3-hexanone
Methyl 4-hydroxy-7,8-diMethylquinoline-2-carboxylate
2-Naphthyl benzoate, 98%
2-Methylindole
Stearyl Acrylate (stabilized with MEHQ)
Garlic Oil
p-Tolylmethyldichlorosilane
5-Vinylpyrrolidone
5-Methylindolin-2-one
PTGES ELISA Kit
CX3CR1 ELISA Kit
HBsAg ELISA Kit
Human LOR ELISA Kit
Monkey CRP (C-reactive protein) ELISA Kit
IL-22 ELISA Kit
VCAM1 ELISA Kit
Human RETNLβ ELISA Kit
Human elongin A,EloA ELISA Kit
C1orf85 ELISA Kit
N.gonorrhoeae Agar Medium Base
GC Agar Base
Hydantoin-5-acetic acid, 98%
Ethyl trans-2-hexenoate, 98%
4-Ethoxystyrene, 97%, stab. with 0.1% 4-tert-butylcatechol
2-Phenyl-2-propanol, 98+%
5-methyl-1H-indole-3-ethylamine monohydrochloride
Methyldiethoxyphosphine
ethyl (S)-(+)-mandelate
iodipamide

1,2,2,6,6-PENTAMETHYLPIPERIDINE
DIACETOXYDIMETHYLSILANE
N-ButylMorpholine
VanadiuM(IV) oxide, 99% (Metals basis)
2-Chloro-6-Methylpyrazine, 97%
1,3-Dichloro-2-propanol, 98+%
Z-Thr-OH
N-Acetyl 2-fluoro-4-(3-hydroxy-3-Methylbut-1-ynyl)aniline
2-Ethoxy-3-forMyl-5-Methylphenylboronic acid
4-Nitrophenyl forMate
Cyclohexene
4-BroMo-1,2-(Methylenedioxy)benzene
3-Bromo-5-methoxybenzoic acid 99%
2-Methyl-6-nitrobenzoic Anhydride
Methyl 2-(3-fluorophenyl)acetate