MKN74胃癌细胞系(货号：GD-C518518)

MKN74胃癌细胞系
产品货号：GD-C518518
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
【悬浮型细胞的传代操作】1)吸出细胞培养液，放入离心管中，离心1000rpm 5分钟；2)吸掉上清液，加入适量之新鲜培养基，混和均匀后，依稀释比例转移至新的培养瓶中，以正常培养条件培养。【注意事项】1)严格的无菌操作；2)适度消化：消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化。附：EDTA(0.02％乙二胺四乙酸二钠)消化液配方：EDTA 0.20g，NaCl 8.00g，KCl 0.20g，KH2PO4 0.02g，葡萄糖 2.00g，0.5％酚红4ml，加入蒸馏水定容至1000ml。10磅20min高压灭菌，使用时调节PH值到7.4。注意EDTA不能被血清中和，使用后培养瓶要彻底清洗，否则再培养时细胞容易脱壁。
3-AzetidineMethanol hydrochloride, 95%
EthoxytriMethylsilane
(5-(Methylthio)pyridin-3-ylthio)Methylboronic acid
2-Chloro-5-fluoropyridine
Tris(1,1,1,3,3,3-hexafluoro-2-propyl) phosphite
6-Chloro-3,4-bipyridine, 95%
7-DiethylaMino-4-MethylcouMarin
Perfluoro-3,6,9-trioxaundecane-1,11-dioic acid
3-(2-Chlorophenyl)isoxazole-5-amine ,97%
Barium(II) 2,2,6,6-tetramethylheptane-3,5-dionate hydrate 99.9%
Zinc bis(trifluoromethylsulfonyl)imide, min. 97%
5-Bromothiophenesulfonyl chloride
Triphenylsulfonium chloride
1-BROMO-2,4-DICHLOROBENZENE
2-Formylbenzeneboronic acid
DiMethyl carbonate
DiMethylthiophosphinoyl Chloride
4-(3-BroMo-5-nitropyridin-2-yl)Morpholine
SaMariuM fluoride, 99.9%, (trace Metal basis)
L-Aspartic Acid SodiuM Salt, Monohydrate
3,4-Diethoxy-3-cyclobutene-1,2-dione
3'-(Trifluoromethyl)acetophenone
D-glucosaminic acid
cyclopropane-1,1-dicarboxylic acid
2,2'-thio-bis(4,6-dichlorophenol)
3-Chlorophenyl isocyanate
Sodium omadine
4'-BROMO-2,2,2-TRIFLUOROACETOPHENONE
4-Methylbenzyl acetate, 98+%
N-Boc-1,4-diaMinobutane, 98%
2-Fluoro-4-(trifluoroMethyl)aniline
4-(4-Morpholinyl)benzeneboronic acid pinacol ester
2,3-Dihydroxybenzoic acid
TRANS-2-PHENYLCYCLOPROPYLAMINE HYDROCHLORIDE
3-Ethylpyridine
Cyclopropyl phenyl ketone
1-(2-N-Boc-Aminoethyl)piperazine
3-Amino-4-bromobenzotrifluoride
Cadmium selenide (99.999+%-Cd) PURATREM
N2-Cyclopropyl-3-nitropyridin-2-amine , 97%
4-TRIFLUOROMETHYL DIPHENYL SULFIDE
4-CHLORO-3-(TRIFLUOROMETHYL)BENZOIC ACID
2-Acetylbenzofuran
BroMophenol red;BPR
Methyl thiosalicylate
3-Anilino-1-propanol
5-AMino-2,4-difluorophenylboronic acid, HCl
4-Ethylphenol, 97%
[1-(Pyrid-3-ylMethyl)piperid-4-yl]Methanol, 97%
QUINACRINE DIHYDROCHLORIDE DIHYDRATE
[6]-Gingerol
N-Benzoyl-N-Phenylhydroxylamine
Ethyl 6-aminoimidazo[1,2-a]pyridine-2-carboxylate
Isooctyl Nitrate
Carboxysulfophenylpyrazolonesodiumsalt; 98%
1H-TETRAZOLE
1-(2-CYANOETHYL)-2-UNDECYLIMIDAZOLE
HEXABROMOSTEARIC ACID
2-Quinoxalinecarboxylic acid
DL-alpha-Methyltryptamine, 99%
Sedoheptulosan Monohydrate
3-[(4-Chlorophenyl)carbamoyl]benzeneboronic acid 98%
4-bromo-1-(2-chloroethyl)-3-methyl-1H-pyrazole(SALTDATA: FREE)
2,2-BIPIPERIDINE
LANTHANUM SULFATE
DIOCTYL PHENYLPHOSPHONATE
D(-)-Fructose
Isonipecotic acid hydrochloride
(R)-(+)-Tetrahydro-2-furoic acid
3-Ethyl-4-Methyl-3-pyrrolin-2-one
3-(4-Chlorophenyl)-1h-pyrazole-5-carboxylic acid
Aza-12-crown-4, 99.5+%
OXYCHLORDANE
1,3-PhenylenediaMine-4-sulfonic Acid
3-Cyano-2-methylpyridine
Dysprosium nitrate
[1-(aminomethyl)cyclopentyl]methanol
phenylsilane
ACF ELISA Kit
LIFR ELISA Kit
Human TGFα ELISA Kit
CK-HMW ELISA Kit
Fbg ELISA Kit
Rat Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen,ⅠCTP ELISA Kit
FABP4 ELISA Kit
PK ELISA Kit
Human adaptor molecule apoptotic peptidase activating factor 1,APAF1 ELISA Kit
CTACK ELISA Kit
MIAC Agar Base
Ferrous sulfate Agar
n-Tricosane, 99%
Methyl cyclopropanecarboxylate, 98%
t-Butyl-5-Norbornene-2-Carboxylate
Ethyl 2-furoate, 99%
3'-(Trifluoromethyl)acetophenone
3-Bromo-2-nitroaniline
ethyl 5-bromofuran-2-carboxylate
benzyl isobutyrate

Benzyl glycinate hydrochloride
D-NORVALINOL
4-Fluoro-2-Hydroxyacetophenone
3,5-Di-tert-butylsalicylic acid, 97%
4-(4-MorpholinylMethyl)benzoic acid, 97%
(+)－Catechin Hydrate
Phthaloyl chloride
4-(TrifluoroMethoxy)benzenesulfonaMide
Sandalwood oil
CYCLOPENTYLACETIC ACID
3-Methoxy-2-naphthol
3-Bromo-4-chlorobenzoic acid
9-Decen-1-ol
1-(2-Bromo-ethoxy)-3,5-dimethyl-benzene
4-Phenoxypyridine