His tag聚组氨酸抗体标签单克隆抗体_详细资料

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保存条件： Store at -20 °C 浓度： 1mg/ml 应用范围： WB=1:1000-5000 IP=1:20-100 （石蜡切片需做抗原修复）形态： Lyophilized or Liquid 亚型： IgG 免疫原： KLH conjugated HHHHHH peptide: 适应物种： Human, fusion protein HHHHHH tagged 宿主： Rabbit 抗体名：聚组氨酸抗体标签单克隆抗体抗体英文名： His tag 保质期： 1年抗原来源： Rabbit 克隆性：多克隆目录编号： LM-33004M 数量：大量供应商：上海联迈生物工程有限公司规格： 50ul  100ul  200ul

His tag聚组氨酸抗体标签单克隆抗体

英文名称 His tag

中文名称聚组氨酸抗体标签单克隆抗体

别    名 HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.

规格价格 50ul/580元购买    100ul/920元购买    200ul/1600元购买    大包装/询价

说明书 50ul  100ul  200ul

研究领域细胞生物  免疫学

抗体来源 Mouse

克隆类型 Monoclonal

克隆号 6E6

交叉反应 Human, fusion protein HHHHHH tagged

产品应用 WB=1:1000-5000 IP=1:20-100 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

性    状 Lyophilized or Liquid

浓    度 1mg/ml

免疫原 KLH conjugated HHHHHH peptide:

亚    型 IgG

纯化方法 affinity purified by Protein G

储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed

产品介绍 background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。
2）可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括：细菌，杆状病毒和哺乳动物）
4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。
5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。

产品图片
Sample:
Lane1:Recombinant human JSRV-ENV Protein (Human) at 100 ng
Lane2:Recombinant human JSRV-ENV Protein (Human) at 100 ng
Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35-45 kD
Observed band size: 40 kD

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His tag聚组氨酸抗体标签单克隆抗体

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英文名称	His tag
中文名称	聚组氨酸抗体标签单克隆抗体
别名	HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
规格价格	50ul/580元购买 100ul/920元购买 200ul/1600元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	细胞生物免疫学
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	6E6
交叉反应	Human, fusion protein HHHHHH tagged
产品应用	WB=1:1000-5000 IP=1:20-100 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated HHHHHH peptide:
亚型	IgG
纯化方法	affinity purified by Protein G
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 2）可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括：细菌，杆状病毒和哺乳动物） 4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。 5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
产品图片	Sample: Lane1:Recombinant human JSRV-ENV Protein (Human) at 100 ng Lane2:Recombinant human JSRV-ENV Protein (Human) at 100 ng Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35-45 kD Observed band size: 40 kD