EP4前列腺素E受体蛋白4抗体_详细资料

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保存条件： Store at -20 °C 浓度： 1mg/ml 应用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）形态： Lyophilized or Liquid 亚型： IgG 免疫原： KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4:151-250/488 <Extracellular> 适应物种： Human, Bee, 宿主： Rabbit 抗体名：前列腺素E受体蛋白4抗体抗体英文名： EP4 保质期： 1年抗原来源： Rabbit 克隆性：多克隆目录编号： LM-8538R 数量：大量供应商：上海联迈生物工程有限公司规格： 100ul  200ul

EP4前列腺素E受体蛋白4抗体

英文名称 EP4

中文名称前列腺素E受体蛋白4抗体

别    名 Prostaglandin E Receptor EP4; EP 4; EP4; EP-4; EP4R; MGC126583; PE2R4_HUMAN; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostaglandin E receptor 4; Prostaglandin E receptor 4 subtype EP4; Prostaglandin E2 receptor; Prostaglandin E2 receptor EP4 subtype; Prostanoid EP4 receptor; PTGER 2; PTGER 4; PTGER2; PTGER4.

Specific References  (2)     |     bs-8538R has been referenced in 2 publications.
[IF=2.38] Hu, Ruiqi, et al. "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." Prostaglandins & Other Lipid Mediators (2016).  IHC-P ;  Others.
PubMed:27246901
[IF=4.50] Koyama, Hiroyuki, et al. "Comprehensive Profiling of GPCR Expression in Ghrelin-producing Cells." Endocrinology (2016): en-2016.  IHC-P ;  Mouse.
PubMed:26671185

规格价格 100ul/1380元购买    200ul/2200元购买    大包装/询价

说明书 100ul  200ul

研究领域信号转导  G蛋白偶联受体

抗体来源 Rabbit

克隆类型 Polyclonal

交叉反应 Human, Bee,

产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量 53kDa

细胞定位细胞膜

性    状 Lyophilized or Liquid

浓    度 1mg/ml

免疫原 KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4:151-250/488 <Extracellular>

亚    型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed

产品介绍 background:
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Function:
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Subunit:
Interacts with FEM1A.

Subcellular Location:
Cell membrane.

Tissue Specificity:
High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Post-translational modifications:
Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P35408

Gene ID:
5734

Database links:
Entrez Gene: 5734 Human

Entrez Gene: 19219 Mouse

Entrez Gene: 84023 Rat

Entrez Gene: 443222 Sheep

Omim: 601586 Human

SwissProt: P35408 Human

SwissProt: P32240 Mouse

SwissProt: P43114 Rat

Unigene: 199248 Human

Unigene: 18509 Mouse

Unigene: 16062 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-EP4 (bs-8538R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-EP4 (bs-8538R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-EP4 antibody (bs-8538R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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EP4前列腺素E受体蛋白4抗体

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英文名称	EP4
中文名称	前列腺素E受体蛋白4抗体
别名	Prostaglandin E Receptor EP4; EP 4; EP4; EP-4; EP4R; MGC126583; PE2R4_HUMAN; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostaglandin E receptor 4; Prostaglandin E receptor 4 subtype EP4; Prostaglandin E2 receptor; Prostaglandin E2 receptor EP4 subtype; Prostanoid EP4 receptor; PTGER 2; PTGER 4; PTGER2; PTGER4.
	Specific References (2) \| bs-8538R has been referenced in 2 publications. [IF=2.38] Hu, Ruiqi, et al. "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." Prostaglandins & Other Lipid Mediators (2016). IHC-P ; Others. PubMed:27246901 [IF=4.50] Koyama, Hiroyuki, et al. "Comprehensive Profiling of GPCR Expression in Ghrelin-producing Cells." Endocrinology (2016): en-2016. IHC-P ; Mouse. PubMed:26671185
规格价格	100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	100ul 200ul
研究领域	信号转导 G蛋白偶联受体
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Bee,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	53kDa
细胞定位	细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4:151-250/488 <Extracellular>
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Function: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Subunit: Interacts with FEM1A. Subcellular Location: Cell membrane. Tissue Specificity: High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle. Post-translational modifications: Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35408 Gene ID: 5734 Database links: Entrez Gene: 5734 Human Entrez Gene: 19219 Mouse Entrez Gene: 84023 Rat Entrez Gene: 443222 Sheep Omim: 601586 Human SwissProt: P35408 Human SwissProt: P32240 Mouse SwissProt: P43114 Rat Unigene: 199248 Human Unigene: 18509 Mouse Unigene: 16062 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-EP4 (bs-8538R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-EP4 (bs-8538R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-EP4 antibody (bs-8538R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.