Human Alpha-Endorphin ELISA Kit (Competitive EIA) - LS-F32273

Human Alpha-Endorphin ELISA Kit (Competitive EIA) - LS-F32273
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LS-F32273-1
1 plate
$695 USD
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SpecificationsPublicationsReviewsImagesPopular Alpha-Endorphin Elisa Kits
Human Alpha-Endorphin ELISA Kit (Competitive EIA) - LS-F25035
Type: Competitive EIA
Reactivity: Human
Range: 12.35-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma

Mouse Alpha-Endorphin ELISA Kit (Competitive EIA) - LS-F25036
Type: Competitive EIA
Reactivity: Mouse
Range: 12.35-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

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Product DescriptionLS-F32273 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Alpha-Endorphin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Alpha-Endorphin as low as 9.375 picograms per milliliter.
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SpecificationsType
 
Competitive EIA (enzyme immunoassay) kit
Target
 
Alpha-Endorphin
Reactivity
 
Human
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
15.625 - 1000 pg/ml
Sensitivity
 
9.375
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Detection Antibody Diluent
• Biotinylated Detection Antibody
• HRP-Streptavidin Conjugate
• HRP-Streptavidin Conjugate Diluent
• Sample Diluent
• TMB Substrate
• Stop Solution
• Wash Buffer (25x)
• Standard (Lyophilized)
• Adhesive Plate Sealer

Background
 
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with a-Endorphin . During the reaction, a-Endorphin in the sample or standard competes with a fixed amount of a-Endorphin on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to a-Endorphin . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of a-Endorphin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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