Sheep Activin B ELISA Kit (Sandwich ELISA) - LS-F32242

Sheep Activin B ELISA Kit (Sandwich ELISA) - LS-F32242
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LS-F32242-1
1 plate
$695 USD
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SpecificationsPublicationsReviewsImagesPopular Activin B Elisa Kits
Human Activin B ELISA Kit (Sandwich ELISA) - LS-F22970
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 15.63-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Rat Activin B ELISA Kit (Competitive EIA) - LS-F24988
Type: Competitive EIA
Reactivity: Rat
Range: 12.35-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma

Human Activin B ELISA Kit (Sandwich ELISA) - LS-F25151
Type: Sandwich ELISA
Reactivity: Human
Range: 15.63-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Human Activin B ELISA Kit (CLIA) - LS-F29971
Type: Quantitative Sandwich CLIA ELISA
Format: 12 x 8-Well Microstrips
Reactivity: Human
Range: 7.813-500 pg/ml
Detection:Chemiluminescent
Sample Types: Serum, Plasma

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Product DescriptionLS-F32242 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Sheep Activin B in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Activin B as low as 14.063 picograms per milliliter.
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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Activin B
Reactivity
 
Sheep
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
23.438 - 1500 pg/ml
Sensitivity
 
14.063
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Detection Antibody Diluent
• Biotinylated Detection Antibody (100x)
• HRP-Streptavidin Conjugate (100x)
• HRP-Streptavidin Conjugate Diluent
• Sample Diluent
• TMB Substrate
• Stop Solution
• Wash Buffer (25x)
• Standard (Lyophilized)
• Adhesive Plate Sealers

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ACVB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ACVB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ACVB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ACVB can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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