Adipose Triglyceride Lipase脂肪甘油三酯脂酶抗体

货号： LM-3831R 保存条件： Store at -20 °C 浓度： 1mg/ml 应用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复） 形态： Lyophilized or Liquid 亚型： IgG 免疫原： KLH conjugated synthetic peptide derived from human ATGL:401-504/504 适应物种： Human, Mouse, Rat, 宿主： Rabbit 抗体名： 脂肪甘油三酯脂酶抗体 抗体英文名： Adipose Triglyceride Lipase 保质期： 1年 抗原来源： Rabbit 克隆性： 多克隆 目录编号： LM-3831R 数量： 大量 供应商： 上海联迈生物工程有限公司 规格： 50ul  100ul  200ul

Adipose Triglyceride Lipase脂肪甘油三酯脂酶抗体

英文名称	Adipose Triglyceride Lipase
中文名称	脂肪甘油三酯脂酶抗体
别    名	PEDF R; Adipose Triglyceride Lipase; TTS 2.2; PEDFR; PEDF-R; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Desnutrin; EC 3.1.1.3; IPLA2 zeta; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Triglyceride hydrolase; TTS2; TTS2.2; ZETA.
规格价格	50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	50ul  100ul  200ul
研究领域	免疫学  生长因子和激素  糖尿病
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	55kDa
细胞定位	细胞膜
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human ATGL:401-504/504
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals. Subunit: Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity. Subcellular Location: Lipid droplet. Cell membrane. Tissue Specificity: Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). Similarity: Contains 1 patatin domain. SWISS: Q96AD5 Gene ID: 57104 Database links: Entrez Gene: 57104 Human Entrez Gene: 66853 Mouse Entrez Gene: 361676 Rat Omim: 609059 Human SwissProt: Q96AD5 Human SwissProt: Q8BJ56 Mouse SwissProt: P0C548 Rat Unigene: 654697 Human Unigene: 29998 Mouse Unigene: 19196 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.  Pathway: Glycerolipid metabolism; triacylglycerol degradation.
产品图片	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 55 kD Observed band size: 55 kD Blank control: A431.  Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (bs-3831R)  Dilution: 3μg /10^6 cells;  Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test.  Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.