LS-F34461 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Monkey APOE / Apolipoprotein E in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of APOE / Apolipoprotein E as low as 3.75 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
6.25 - 400 ng/ml
3.75
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Sample Diluent
TMB Substrate
Stop Solution
Wash Buffer (25x)
Standard (Lyophilized)
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ApoE antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ApoE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ApoE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofApoE can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.
About APOE / Apolipoprotein EP02649 NM_000041 NP_000032.1
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. (More About APOE / Apolipoprotein E)
Monkey APOE / Apolipoprotein E ELISA Kit (Sandwich ELISA) - LS-F34461
Rat Apolipoprotein C-I ELISA Kit (Sandwich ELISA) - LS-F38960
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