LS-F33211 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human AMH / Anti-Mullerian Hormone in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of AMH / Anti-Mullerian Hormone as low as 28.125 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
46.875 - 3000 pg/ml
28.125
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Sample Diluent
TMB Substrate
Stop Solution
Wash Buffer (25x)
Standard (Lyophilized)
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AMHantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AMH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMH can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.
About AMH / Anti-Mullerian HormoneP03971 NM_000479 NP_000470.2
Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. (More About AMH / Anti-Mullerian Hormone)
Human AMH / Anti-Mullerian Hormone ELISA Kit (Sandwich ELISA) - LS-F33211
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