MAP1D蛋氨酸氨肽酶1D抗体_详细资料

产品信息

保存条件： Store at -20 °C 浓度： 1mg/ml 应用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）形态： Lyophilized or Liquid 亚型： IgG 免疫原： KLH conjugated synthetic peptide derived from human MAP1D:251-335/335 适应物种： Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep, 宿主： Rabbit 抗体名：蛋氨酸氨肽酶1D抗体抗体英文名： MAP1D 保质期： 1年抗原来源： Rabbit 克隆性：多克隆目录编号： LM-18659R 数量：大量供应商：上海联迈生物工程有限公司规格： 100ul  200ul

MAP1D蛋氨酸氨肽酶1D抗体

英文名称 MAP1D

中文名称蛋氨酸氨肽酶1D抗体

别    名 AMP1D_HUMAN; CDS of metAP 3 within PCR fragment; M ethionine aminopeptidase 1D, mitochondrial precursor; MAP 1D; MAP1D; MetAP 1D; Metap1l; Methionine aminopeptidase 1D; Methionine aminopeptidase 1D, mitochondrial; Methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial; mitochondrial; Peptidase M 1D.

规格价格 100ul/1380元购买    200ul/2200元购买    大包装/询价

说明书 100ul  200ul

研究领域细胞生物  转录调节因子  表观遗传学

抗体来源 Rabbit

克隆类型 Polyclonal

交叉反应 Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep,

产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量 35kDa

细胞定位细胞浆

性    状 Lyophilized or Liquid

浓    度 1mg/ml

免疫原 KLH conjugated synthetic peptide derived from human MAP1D:251-335/335

亚    型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed

产品介绍 background:
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Function:
Removes the amino-terminal methionine from nascent proteins (By similarity). May play a role in colon tumorigenesis.

Subcellular Location:
Mitochondrion.

Tissue Specificity:
Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).

Similarity:
Belongs to the peptidase M24A family.

SWISS:
Q6UB28

Gene ID:
254042

Database links:
Entrez Gene: 254042 Human

Entrez Gene: 66559 Mouse

Entrez Gene: 311748 Rat

Omim: 610267 Human

SwissProt: Q6UB28 Human

SwissProt: Q9CPW9 Mouse

Unigene: 298250 Human

Unigene: 152796 Mouse

Unigene: 24026 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-MAP1D (bs-18659R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (bs-18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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MAP1D蛋氨酸氨肽酶1D抗体

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英文名称	MAP1D
中文名称	蛋氨酸氨肽酶1D抗体
别名	AMP1D_HUMAN; CDS of metAP 3 within PCR fragment; M ethionine aminopeptidase 1D, mitochondrial precursor; MAP 1D; MAP1D; MetAP 1D; Metap1l; Methionine aminopeptidase 1D; Methionine aminopeptidase 1D, mitochondrial; Methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial; mitochondrial; Peptidase M 1D.
规格价格	100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	100ul 200ul
研究领域	细胞生物转录调节因子表观遗传学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	35kDa
细胞定位	细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human MAP1D:251-335/335
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008] Function: Removes the amino-terminal methionine from nascent proteins (By similarity). May play a role in colon tumorigenesis. Subcellular Location: Mitochondrion. Tissue Specificity: Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Similarity: Belongs to the peptidase M24A family. SWISS: Q6UB28 Gene ID: 254042 Database links: Entrez Gene: 254042 Human Entrez Gene: 66559 Mouse Entrez Gene: 311748 Rat Omim: 610267 Human SwissProt: Q6UB28 Human SwissProt: Q9CPW9 Mouse Unigene: 298250 Human Unigene: 152796 Mouse Unigene: 24026 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-MAP1D (bs-18659R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (bs-18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.