英文名称 | Keratin 36 |
中文名称 | 细胞角蛋白36抗体 |
别 名 | HA6; Hair keratin; Hair keratin, type I Ha6; HHA6; HKA6; K36; Keratin; Keratin hair acidic 6; Keratin type I cuticular Ha6; keratin, hair, acidic, 6; Keratin, hard, type I; Keratin, hard, type I, 6; keratin, type I cuticular Ha6; Keratin-36; Krt36; KRT36_HUMAN; KRTHA6; type I cuticular Ha6; type I Ha6. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 细胞生物 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 52kDa |
细胞定位 | 细胞浆 细胞外基质 分泌型蛋白 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Keratin 36:301-400/467 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008] Subunit: Heterotetramer of two type I and two type II keratins. Tissue Specificity: Expressed in the hair follicle. Similarity: Belongs to the intermediate filament family. SWISS: O76013 Gene ID: 8689 Database links: Entrez Gene: 8689 Human Entrez Gene: 520668 Cow Entrez Gene: 16673 Mouse Omim: 604540 Human SwissProt: O76013 Human SwissProt: B1AQ75 Mouse Unigene: 248189 Human Unigene: 306829 Mouse Unigene: 401866 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |  Sample: HL-60 Cell (Human) Lysate at 30 ug Primary: Anti- Keratin (bs-16999R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 60 kD  Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Keratin 36) Polyclonal Antibody, Unconjugated (bs-16999R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |