EXO-FBS-50A-1Systembio Exosome-depleted FBS （无外泌体胎牛血清）

EXO-FBS-50A-1Systembio Exosome-depleted FBS （无外泌体胎牛血清）

产品关键词：GibcoA2720801无外泌体胎牛血清Fetal Bovine Serum, exosome-depleted；Thermo无外泌体胎牛血清A2720801;Gibco胎牛血清(澳洲)10099141；Gibco南美胎牛血清10270106；Gibco南美FBS盒装A31608；Gibco FBS(北美)16000-044；Gibco Fetal Bovine Serum, qualified, South America origin. Gibco FBS, qualified, South America origin；EXO-FBS-50A-1-SBI无外泌体胎牛血清；GibcoA2720801无外泌体胎牛血清Fetal Bovine Serum, exosome-depleted

产品描述

当外泌体在1980年首次被发现后，其被认为是细胞排泄废物的一种方式，Z近的研究发现外泌体在很多生理病理上起着重要的作用，这些发现点燃了人们对细胞分泌膜泡的兴趣。exosome及其他细胞外囊泡正起着不可缺少的细胞通讯作用。在肿瘤发展过程中，exosome介导了关键的步骤，如刺激血管生成，削弱免yi反应，甚至参与了转移前微环境的形成。在正常的生理过程中，exosome也起了重要作用，如胎盘exosome帮助形成对母亲免yi系统的免yiYZ屏障。总之，在正常和病理的条件下，细胞都释放小囊泡，以各种方式影响其他细胞。

无外泌体血清可以特异性激发一些细胞反应，表明它们可以在作为zhi疗递送载体方面有巨大的潜力。无外泌体血清的囊泡性质使得它们适合作为用于yao物或核酸递送的潜在纳米载体。在这里，研究人员需要解决的问题是，无外泌体血清的大小分布也会影响外泌体的zhi疗潜力。

1：利用聚合物沉淀分离的外泌体比超离法的粒子分布要小。

2：划痕实验证实聚合物沉淀所得的小粒径的外泌体被细胞摄取后迁移更快。

产品应用                         

EXO-FBS-50A-1Systembio Exosome-depleted FBS （无外泌体胎牛血清）操作手册：Exo-FBS User Manual (PDF) »

对比

1):Exo-FBS Has Greatly Reduced Levels of Bovine Exosomes

2):Exo-FBS is Depleted of Bovine CD63 Exosomes

3):Exo-FBS is Cleaner than Ultracentrifuged FBS

4):Exo-FBS Has Undetectable Levels of Bovine microRNAs

5):Exo-FBS Is Identical to Standard FBS for Cell Growth

 

Exo-FBS Has Greatly Reduced Levels of Bovine Exosomes

    

Use SBI’s Exo-FBS to ensure your exosome preparations from cell or tissue culture media contain only the exosomes from your cells, not from the FBS in your media. Both ELISA and NanoSight analysis demonstrate the great reduction in bovine exosome levels in Exo-FBS compared to standard FBS.

NanoSight Particle Analysis Show Low Exosome Levels in Exo-FBS

To demonstrate the depletion of exosomes in our Exo-FBS product, we diluted standard FBS and Exo-FBS samples 1:1000 and then analyzed for particle size and abundance using a NanoSight LM10 instrument. The standard FBS sample shows a significant amount of exosome-sized microvesicles where the Exo-FBS exosome-depleted FBS sample has a drastic reduction in exosome particles.

Exo-FBS is Depleted of Bovine CD63 Exosomes

    

The tetraspanin CD63 protein is a common marker for exosomes. We utilized a bovine-specific anti-CD63 antibody to develop an Enzyme Linked Immunosorbent Assay (ELISA). Equal volumes (50 µl) of either standard FBS or Exo-FBS depleted media supplement were used in this ELISA assay. Amounts of CD63-positive bovine exosomes are dramatically reduced in Exo-FBS compared to standard FBS (graphed results are normalized to the signal level of standard FBS).   

Exo-FBS is Cleaner than Ultracentrifuged FBS

Quality Control data is generated on every batch of Exo-FBS produced at SBI by comparing NanoSight particle count analyses to the source FBS, FBS ultracentrifuged for 18 hours and SBI's exosome-depleted Exo-FBS products. All samples were diluted 1:100 and NTA data collected in triplicate.

Conclusion

With Exo-FBS, you can be confident that the exosomes you are isolating are generated by the cells in your culture and not contaminants from your culture medium.

Exo-FBS Has Undetectable Levels of Bovine microRNAs

Not only does Exo-FBS have greatly reduced levels of exosomes, bovine microRNAs in Exo-FBS are undetectable as shown by highly sensitive qPCR assays. Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to cDNA and 72 individual bovine microRNAs were measured by qPCR using SBI's QuantiMir system. Of the 72 microRNAs tested, 12 yielded amplification curves in the FBS sample but not in the Exo-FBS sample.

Exo-FBS Is Identical to Standard FBS for Cell Growth

Not only does Exo-FBS enable confident isolation of exosomes free from contaminants present in standard FBS, it supports identical growth rates as standard FBS.  
To compare growth rates of cells in Exo-FBS versus standard FBS, we grew HT1080 fibrosarcoma cells, PC-3 prostate cancer cells, MCF-7 breast cancer cells, and HEK293 cells in complete medium with either 10% standard FBS or 10% Exo-FBS supplement. Cells were seeded at either 10,000 or 20,000 cells, and then cultured under standard conditions at 37°C with 5% CO2 for 5 days in the medium indicated. The cells were imaged calculate growth rates and observe cellular morphologies. Equivalent growth and similar cellular morphologies were observed for standard FBS and Exo-FBS media tested across these 4 cell lines.

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