NucView® 488 Caspase-3 Substrate

NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time. 

Unlike conventional caspase assays, NucView® 488 Caspase-3 Substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView® 488 Caspase-3 Substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining.

NucView® 488 Caspase-3 Substrate, is also offered as a 1 mM solution in PBS (phosphate-buffered saline) (catalog no. 10403) for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® 488 staining. In addition to stand-alone substrate stock solutions, we offer a NucView® 488 assay kit with detailed application protocols. Biotium also offers the blue fluorescent NucView® 405 Caspase-3 Substrate for use with the 405 nm laser line and the orange fluorescent NucView® 530 for detection in the Cy3® channel.