OBSCN antibody_详细资料_供应商-仪器网

产品信息

保存条件： -20 °C 浓度： 1mg/ml 应用范围： ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 形态：冻干粉或液体亚型： IgG 免疫原： KLH conjugated synthetic peptide derived from human OBSCN:101-200/7963 适应物种： Human, Mouse, Rat, 宿主： Rabbit 抗体名：遮蔽蛋白肌球蛋白轻链激酶抗体抗体英文名： OBSCN antibody 保质期： 12个月抗原来源： Rabbit 克隆性：多克隆目录编号： kl-21129R 数量：大量供应商：上海康朗生物科技有限公司规格： 100ul 200ul

OBSCN antibody

中文名称遮蔽蛋白肌球蛋白轻链激酶抗体

别    名 BC046431; Gm878; KIAA1556; KIAA1639; OBSCN; OBSCN_HUMAN; Obscurin; Obscurin-MLCK; Obscurin-myosin light chain kinase; Obscurin-RhoGEF; OTTMUSG00000005786; UNC89.

规格价格 100ul/1380元购买    200ul/2200元购买    大包装/询价

说明书 100ul  200ul

研究领域细胞生物  发育生物学  信号转导

抗体来源 Rabbit

克隆类型 Polyclonal

交叉反应 Human, Mouse, Rat,

产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量 868kDa

细胞定位细胞浆

性    状 Lyophilized or Liquid

浓    度 1mg/ml

免疫原 KLH conjugated synthetic peptide derived from human OBSCN:101-200/7963

亚    型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed

产品介绍 background:
The obscurin gene spans more than 150 kb, contains over 80 exons and encodes a protein of approximately 720 kDa. The encoded protein contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains. This protein belongs to the family of giant sacromeric signaling proteins that includes titin and nebulin, and may have a role in the organization of myofibrils during assembly and may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Function:
Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.

Subcellular Location:
Cytoplasm > myofibril > sarcomere > M line. Cytoplasm > myofibril > sarcomere > Z line. In differentiating skeletal muscle cells, isoform 3 primarily localizes to the sarcomeric M-line and less frequently to the Z-disk. Isoform 3 colocalizes with ANK1 isoform Mu17/ank1.5 at the M-line in differentiated skeletal muscle cells.

Similarity:
Belongs to the protein kinase superfamily.
CAMK Ser/Thr protein kinase family.
Contains 1 DH (DBL-homology) domain.
Contains 3 fibronectin type-III domains.
Contains 55 Ig-like (immunoglobulin-like) domains.
Contains 1 IQ domain.
Contains 2 PH domains.
Contains 2 protein kinase domains.
Contains 1 SH3 domain.

SWISS:
Q5VST9

Gene ID:
84033

Database links:
Entrez Gene: 84033 Human

Omim: 608616 Human

SwissProt: Q5VST9 Human

Unigene: 650039 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OBSCN) Polyclonal Antibody, Unconjugated (bs-21129R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

您可能感兴趣的产品

OBSCN antibody

产品信息

联系我们

关注我们

中文名称	遮蔽蛋白肌球蛋白轻链激酶抗体
别名	BC046431; Gm878; KIAA1556; KIAA1639; OBSCN; OBSCN_HUMAN; Obscurin; Obscurin-MLCK; Obscurin-myosin light chain kinase; Obscurin-RhoGEF; OTTMUSG00000005786; UNC89.
规格价格	100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	100ul 200ul
研究领域	细胞生物发育生物学信号转导
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat,
产品应用	ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	868kDa
细胞定位	细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human OBSCN:101-200/7963
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The obscurin gene spans more than 150 kb, contains over 80 exons and encodes a protein of approximately 720 kDa. The encoded protein contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains. This protein belongs to the family of giant sacromeric signaling proteins that includes titin and nebulin, and may have a role in the organization of myofibrils during assembly and may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Function: Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils. Subcellular Location: Cytoplasm > myofibril > sarcomere > M line. Cytoplasm > myofibril > sarcomere > Z line. In differentiating skeletal muscle cells, isoform 3 primarily localizes to the sarcomeric M-line and less frequently to the Z-disk. Isoform 3 colocalizes with ANK1 isoform Mu17/ank1.5 at the M-line in differentiated skeletal muscle cells. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 DH (DBL-homology) domain. Contains 3 fibronectin type-III domains. Contains 55 Ig-like (immunoglobulin-like) domains. Contains 1 IQ domain. Contains 2 PH domains. Contains 2 protein kinase domains. Contains 1 SH3 domain. SWISS: Q5VST9 Gene ID: 84033 Database links: Entrez Gene: 84033 Human Omim: 608616 Human SwissProt: Q5VST9 Human Unigene: 650039 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OBSCN) Polyclonal Antibody, Unconjugated (bs-21129R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.