规格: | 250mg | 产品价格: | 262.5 |
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规格: | 500mg | 产品价格: | 399 |
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规格: | 1g | 产品价格: | 525 |
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规格: | 5g | 产品价格: | 2362.5 |
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Catalog Number 17341
Synonyms NBT|p-Nitro Blue Tetrazolium|Nitrotetrazolium Blue|NSC 27622
Molecular Formula C40H30N10O6 • 2Cl
Relative Molecular Mass 817.6
CAS Registry Number 298-83-9
Formulation A crystalline solid
Purity ≥98%
Storage -20°C
SMILES COC1=CC(C2=CC(OC)=C([N+]3=NC(C4=CC=CC=C4)=NN3C5=CC=C([N+]([O-])=O)C=C5)C=C2)=CC=C1[N+]6=NC(C7=CC=CC=C7)=NN6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]
产品描述
Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.
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温馨提示:不可用于临床ZL。