Phospho-Aurora B (Tyr12) antibody_详细资料

产品信息

保存条件： -20 °C 浓度： 1mg/ml 应用范围： ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 形态：冻干粉或液体亚型： IgG 免疫原： KLH conjugated Synthesised phosphopeptide derived from human Aurora B around the phosphorylation site of Tyr12:WP(p-Y)GR 适应物种： Human, Mouse, Rat, Pig, Cow, Horse, Sheep, 宿主： Rabbit 抗体名：磷酸化有丝分裂激酶B抗体抗体英文名： Phospho-Aurora B (Tyr12) antibody 保质期： 12个月抗原来源： Rabbit 克隆性：多克隆目录编号： kl-12552R 数量：大量供应商：上海康朗生物科技有限公司规格： 100ul

Phospho-Aurora B (Tyr12) antibody

中文名称磷酸化有丝分裂激酶B抗体

别    名 Aurora B (phospho Y12); AURKB_HUMAN; Aurora B (phospho T12); AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5.

规格价格 100ul/1580元购买        大包装/询价

说明书 100ul

产品类型磷酸化抗体

研究领域肿瘤  细胞生物  神经生物学  信号转导  激酶和磷酸酶

抗体来源 Rabbit

克隆类型 Polyclonal

交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Sheep,

产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量 39kDa

细胞定位细胞核细胞浆

性    状 Lyophilized or Liquid

浓    度 1mg/ml

免疫原 KLH conjugated Synthesised phosphopeptide derived from human Aurora B around the phosphorylation site of Tyr12:WP(p-Y)GR

亚    型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed

产品介绍 background:
Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation.

Function:
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.

Subcellular Location:
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

Tissue Specificity:
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Post-translational modifications:
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.

DISEASE:
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.

SWISS:
Q96GD4

Gene ID:
9212

Database links:
Entrez Gene: 9212 Human

Entrez Gene: 20877 Mouse

Entrez Gene: 114592 Rat

Omim: 604970 Human

SwissProt: Q96GD4 Human

SwissProt: O70126 Mouse

SwissProt: O55099 Rat

Unigene: 442658 Human

Unigene: 3488 Mouse

Unigene: 10865 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Aurora B (Tyr12)) Polyclonal Antibody, Unconjugated (bs-12552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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中文名称	磷酸化有丝分裂激酶B抗体
别名	Aurora B (phospho Y12); AURKB_HUMAN; Aurora B (phospho T12); AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5.
规格价格	100ul/1580元购买大包装/询价
说明书	100ul
产品类型	磷酸化抗体
研究领域	肿瘤细胞生物神经生物学信号转导激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Pig, Cow, Horse, Sheep,
产品应用	ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	39kDa
细胞定位	细胞核细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from human Aurora B around the phosphorylation site of Tyr12:WP(p-Y)GR
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation. Function: May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. Subcellular Location: Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Tissue Specificity: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Post-translational modifications: Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. DISEASE: Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. Contains 1 protein kinase domain. SWISS: Q96GD4 Gene ID: 9212 Database links: Entrez Gene: 9212 Human Entrez Gene: 20877 Mouse Entrez Gene: 114592 Rat Omim: 604970 Human SwissProt: Q96GD4 Human SwissProt: O70126 Mouse SwissProt: O55099 Rat Unigene: 442658 Human Unigene: 3488 Mouse Unigene: 10865 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Aurora B (Tyr12)) Polyclonal Antibody, Unconjugated (bs-12552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.