JIM4抗体，JIM4 Antibodies，Jim系列抗体_详细资料

产品信息

JIM4抗原是阿拉伯半乳糖蛋白 JIM13 JIM14 JIM15 JIM16的抗原都是阿拉伯半乳糖蛋白 JIM13 JIM14 免疫原是AGP2 JIM15 JIM16的免疫原是AGP1 JIM17抗原是阿拉伯半乳糖 LM10抗原是木质素 LM2免疫原是花粉壁材料抗原是阿拉伯半乳糖蛋白

Antibody Name: JIM4

Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling

Catolog#:

Isotype: IgM

Antibody Type: Monoclonal

Source Animal: Rat

Detail Info for Purified Antibody:

Immunogen: Protoplasts from suspension cultured cells

Antigen: Arabinogalactan glycoprotein

Plant Origin: Carrot (Daucus carota)

Working Concentration: Neat

Status of Antibody: Hybridoma supernatant

Polysaccharide Cross Reactivity: JIM4.pdf

Epitope Structure for Carbonhydrate Antigen: betaGlcA-(1,3)-alphaGalA-(1,2)-Rha

M.W. of Protein Antigen(KDa): 70-100

Protein Cross Reactivity:

References: J. P. Knox, S. Day, and K. Roberts. (1989) A set of cell surface glycoproteins forms an early marker of cell position, but not cell type, in the root apical meristem of Daucus carota L. Development 106:47-56.
E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym.24:281-286.

E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139.

Comments:
JIM4 binds to a set of discreet protein bands MW 20-60 KDa in addition to the smear indicated above. Binding is not competable with monosaccharides. The most competitive oligosaccharide tested to date is the one indicated above. JIM4 also binds to several exudate gums, including gum arabic and gum ghatti.

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Experiment Title:Arabidopsis inflorescence stem expansion glycoprofiling

Experiment Title: Arabidopsis inflorescence stem expansion glycoprofiling

Description:
Series of transverse sections at various regions of a bolting inflorescenc

e primary stem to capture compositional changes during cell wall development.

Organism: Arabidopsis thaliana

Tissues: Inflorescence stem

Treatments: Developmental stage (young, maximum growth rate, and cessation)

Design: Glycoprofiling_Arab_inflr_exp-design.pdf

Antibodies Employed: MAC207
CCRC-M1
CCRC-M2
CCRC-M7
CCRC-M8
CCRC-M9
CCRC-M11
CCRC-M12
CCRC-M13
CCRC-M14
CCRC-M15
CCRC-M16
CCRC-M22
CCRC-M30
CCRC-M31
CCRC-M32
CCRC-M34
CCRC-M36
CCRC-M38
CCRC-M44
CCRC-M48
CCRC-M49
CCRC-M70
JIM1
JIM3
JIM4
JIM5
JIM7
JIM8
JIM11
JIM12
JIM13
JIM14
JIM15
JIM16
JIM17
JIM19
JIM20
JIM84
JIM93
JIM94
JIM101
JIM131
JIM132
JIM133
JIM136
JIM137
LM10
LM18
LM19
LM20
MAC204
MAC265
MAC266
PN 16.4B4

Immunolableing Protocol: Immunolabeling_protocol_for_CCRC.txt

Imageing Protocol: Imaging_protocol_for_CCRC.txt

Contributor Name: Hardy Hall, Jingling Cheung, Brian Ellis

Contributor Address: Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

Related Publication: Immunoprofiling reveals unique cell-specific patterns of wall epitopes in the expanding
Arabidopsis stem (DOI: 10.1111/tpj.12111)

Publication Status: In Press

Related Image Sets: CCRC-M1-Panel
CCRC-M11-panel
CCRC-M12-panel
CCRC-M13-panel
CCRC-M14-panel
CCRC-M15-panel
CCRC-M16-panel
CCRC-M2-panel
CCRC-M22-panel
CCRC-M30-panel
CCRC-M31-panel
CCRC-M32-panel
CCRC-M34-panel
CCRC-M36-panel
CCRC-M38-panel
CCRC-M44-panel
CCRC-M48-panel
CCRC-M49-panel
CCRC-M7-panel
CCRC-M70-panel
CCRC-M8-panel
CCRC-M9-panel
JIM1-panel
JIM101-panel
JIM11-panel
JIM12-panel
JIM13-panel
JIM131-panel
JIM132-panel
JIM133-panel
JIM136-panel
JIM137-panel
JIM14-panel
JIM15-panel
JIM16-panel
JIM17-panel
JIM19-panel
JIM20-panel
JIM3-panel
JIM4-panel
JIM5-panel
JIM7-panel
JIM8-panel
JIM84-panel
JIM93-panel
JIM94-panel
LM10-panel
LM18-panel
LM19-panel
LM20-panel
MAC204-panel
MAC207-panel
MAC265-panel
MAC266-panel
PN 16.4B4-panel

Experiment Title:Arabidopsis inflorescence stem expansion glycoprofiling
Experiment Title:	Arabidopsis inflorescence stem expansion glycoprofiling
Description:	Series of transverse sections at various regions of a bolting inflorescenc e primary stem to capture compositional changes during cell wall development.
Organism:	Arabidopsis thaliana
Tissues:	Inflorescence stem
Treatments:	Developmental stage (young, maximum growth rate, and cessation)
Design:	Glycoprofiling_Arab_inflr_exp-design.pdf
Antibodies Employed:	MAC207 CCRC-M1 CCRC-M2 CCRC-M7 CCRC-M8 CCRC-M9 CCRC-M11 CCRC-M12 CCRC-M13 CCRC-M14 CCRC-M15 CCRC-M16 CCRC-M22 CCRC-M30 CCRC-M31 CCRC-M32 CCRC-M34 CCRC-M36 CCRC-M38 CCRC-M44 CCRC-M48 CCRC-M49 CCRC-M70 JIM1 JIM3 JIM4 JIM5 JIM7 JIM8 JIM11 JIM12 JIM13 JIM14 JIM15 JIM16 JIM17 JIM19 JIM20 JIM84 JIM93 JIM94 JIM101 JIM131 JIM132 JIM133 JIM136 JIM137 LM10 LM18 LM19 LM20 MAC204 MAC265 MAC266 PN 16.4B4
Immunolableing Protocol:	Immunolabeling_protocol_for_CCRC.txt
Imageing Protocol:	Imaging_protocol_for_CCRC.txt
Contributor Name:	Hardy Hall, Jingling Cheung, Brian Ellis
Contributor Address:	Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
Related Publication:	Immunoprofiling reveals unique cell-specific patterns of wall epitopes in the expanding Arabidopsis stem (DOI: 10.1111/tpj.12111)
Publication Status:	In Press
Related Image Sets:	CCRC-M1-Panel CCRC-M11-panel CCRC-M12-panel CCRC-M13-panel CCRC-M14-panel CCRC-M15-panel CCRC-M16-panel CCRC-M2-panel CCRC-M22-panel CCRC-M30-panel CCRC-M31-panel CCRC-M32-panel CCRC-M34-panel CCRC-M36-panel CCRC-M38-panel CCRC-M44-panel CCRC-M48-panel CCRC-M49-panel CCRC-M7-panel CCRC-M70-panel CCRC-M8-panel CCRC-M9-panel JIM1-panel JIM101-panel JIM11-panel JIM12-panel JIM13-panel JIM131-panel JIM132-panel JIM133-panel JIM136-panel JIM137-panel JIM14-panel JIM15-panel JIM16-panel JIM17-panel JIM19-panel JIM20-panel JIM3-panel JIM4-panel JIM5-panel JIM7-panel JIM8-panel JIM84-panel JIM93-panel JIM94-panel LM10-panel LM18-panel LM19-panel LM20-panel MAC204-panel MAC207-panel MAC265-panel MAC266-panel PN 16.4B4-panel

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Antibody Name:	JIM4
Related Experiments:	Arabidopsis inflorescence stem expansion glycoprofiling
Catolog#:
Isotype:	IgM
Antibody Type:	Monoclonal
Source Animal:	Rat
Detail Info for Purified Antibody:
Immunogen:	Protoplasts from suspension cultured cells
Antigen:	Arabinogalactan glycoprotein
Plant Origin:	Carrot (Daucus carota)
Working Concentration:	Neat
Status of Antibody:	Hybridoma supernatant
Polysaccharide Cross Reactivity:	JIM4.pdf
Epitope Structure for Carbonhydrate Antigen:	betaGlcA-(1,3)-alphaGalA-(1,2)-Rha
M.W. of Protein Antigen(KDa):	70-100
Protein Cross Reactivity:
References:	J. P. Knox, S. Day, and K. Roberts. (1989) A set of cell surface glycoproteins forms an early marker of cell position, but not cell type, in the root apical meristem of Daucus carota L. Development 106:47-56. E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym.24:281-286. E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139.
Comments:	JIM4 binds to a set of discreet protein bands MW 20-60 KDa in addition to the smear indicated above. Binding is not competable with monosaccharides. The most competitive oligosaccharide tested to date is the one indicated above. JIM4 also binds to several exudate gums, including gum arabic and gum ghatti.