Fig1: Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: Mouse colon
Lane 3: Rat liver
Fig2: ICC staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.