PHD1/prolyl hydroxylase 重组兔单抗

Fig1: Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC12
Lane 3: NIH/3T3

Fig2: ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig4: ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.

Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.

Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.

Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.

Fig9: Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.