伯乐bio-rad微滴式数字PCR系统QX100

The QX100 Droplet Digital PCR (ddPCR™) system provides an absolute quantification of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. The award-winning QX100 Droplet Digital PCR system is the third generation of PCR technology and provides a revolutionary approach to target DNA quantification.

The QX100 Droplet Digital PCR system's high-level performance lets you:

• Detect rare DNA target copies with unmatched sensitivity
• Measure small fold differences with the highest precision

Droplet Digital PCR Technology and Workflow

The QX100 system consists of two components: the QX100 droplet generator and the QX100 droplet reader. A Bio-Rad thermal cycler equipped with a 96–deep well reaction module is also needed to complete the Droplet Digital PCR workflow.

The QX100 droplet generator partitions samples containing cDNA or RNA template into 20,000 nanoliter-sized droplets. After PCR, droplets from each sample are streamed in single file through the QX100 droplet reader. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form.