重组人基质金属蛋白酶-3 MMP3

MMP3, also known as stromelysin1, can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. MMP3 can also activates procollagenase.MMP3 does not cleave the triple helical region of interstitial collagens, a feature distinguishing the stromelysins from the collagenases. MMP3 contains several distinct domains; a prodomain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin like) domain. MMP3 is crucial in connective tissue remodeling.In addition, MMP3 may be involved in enzyme cascades and cytokine regulation.

·Description
Recombinant Human MMP3 produced by transiently transfected human cell is the proform (Accession#P08254)[Tyr18- Cys477(Lys45Glu)] of human MMP3 fused with a polyhistidine tag at the C-terminus.

·Quality Control

Biological activity:
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, The specific activity is > 150 pmoles/min/μg.

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: Predicted N terminal amino acid is Tyr18.
Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHu MMP3.

·Formulation
Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized rHuMMP3 in sterile water not less than 100μg/ml.

·Storage
Lyophilized rHu MMP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHu MMP3 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.