流程图
DNA 序列分析 ( Terminator chenistry, ABI PRISM 3730测序仪)
AxyPrep 质粒小量试剂盒纯化不同大小的质粒DNA,0.5ugDNA/泳道。
Lane 1:3Kb Lane 2:4 Kb
Lane 3:4.9 Kb Lane 4:5 Kb
Lane 5:5.9 Kb Lane 6:6 Kb
Lane 7:8 Kb Lane 8:10 Kb
M:Axygen 1Kb DNA Ladder
AxyPrep 质粒小量试剂盒纯化质粒pET42.1a(插入片段)
Lane 1,3:pET42.1a (0.3 ug/泳道)
Lane 2,4:EcoR I 酶切(0.3 ug/泳道)
M: Axygen 1Kb DNA Ladder