BM-5010-D
产品描述
Nutragen® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. Nutragen® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
Nutragen® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. Nutragen® collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. Nutragen® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
Nutragen® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Nutragen® collagen is provided in a 50 mL volume and is contained in user-friendly packaging for use and storage. Nutragen® is sterile filtered and is supplied as a ready to use solution.
Nutragen胶原蛋白被称为标准纯度的胶原蛋白(>99.9%胶原蛋白含量),其功能可媲美大多数的原生胶原蛋白。Nutragen提取于来自美国封闭式牛群的牛皮,先进的生物生产过程符合严格的质量标准,已被证明是质量超凡的。Nutragen胶原蛋白是由约97%的I型胶原蛋白,其余的为III型胶原蛋白组成的,它具有很高的单体含量可以用于测试凝胶渗透色谱。本产品的浓度约为6mg/ml提供给极端坚固的凝胶,每个批次的具体浓度会写于产品的COA上随货发出。Nutragen是溶于0.01N盐酸的端胶原蛋白,因此它的PH值约为2。Nutragen胶原蛋白是理想的蛋白质涂层,可用于准备培养的细胞或者固体凝胶。Nutragen胶原蛋白的包装是50ml,便于储存和使用,经过灭菌的溶液随时可以使用。
产品信息
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Nutragen® 胶原蛋白溶液
货号 # BM-5010-D
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灭菌方法
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过滤
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形态
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溶液
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包装尺寸
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50 mL
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储存温度
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2-10 °C
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有效期
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详见产品包装与随货发出的COA
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浓度(缩二脲蛋白测定)
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5.8-6.8 mg/mL
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纯度(胶原蛋白浓度)
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> 99.9%
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pH
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pH约为 2, 0.01 N HCl
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凝胶时间(凝胶时间测定法)
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< 40 分钟
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原纤维形成(纤维形成)
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>0.5 吸光度单位
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聚丙烯酰胺凝胶电泳(SDS–PAGE)
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> 85%含胶原蛋白在α,β,和γ带,< 15%含比α带快的胶原蛋白
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无菌的
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没有细菌生长
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内毒素 (LAL)
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< 1.0 EU/ml
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细胞附着实验
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通过
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Source
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牛皮–胃蛋白酶提取
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