Topoisomerase I Rabbit Monoclonal Antibody_详细资料

产品信息

保存条件： -20℃保存供应商：上海联迈生物工程有限公司保质期：一年英文名： Topoisomerase I Rabbit Monoclonal Antibody 数量：大量规格： 50μl

Topoisomerase I Rabbit Monoclonal Antibody

产品编号产品名称产品包装产品价格

LM2053 Topoisomerase I Rabbit Monoclonal Antibody 50μl 618.00元

来源用途交叉反应性分子量

Rabbit WB, IF, IHC, ICC, FC H, M 91.0kDa

       WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
       H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
       配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
       建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB IP IF IHC ICC FC ELISA ChIP

1:1,000-1:5,000 - 1:50-1:500 1:50-1:200 1:50-1:500 1:50-1:100 - -

抗体详细信息如下:

About this Antibody

Name Topoisomerase I Rabbit Monoclonal Antibody

Category Rabbit Monoclonal Antibody (RabMAb); Primary antibody

Isotype IgG

Purification Affinity purification

About the Immunogen

Immunogen Recombinant protein

Gene ID 7150(Human); 21969(Mouse)

SwissProt P11387(Human); Q04750(Mouse)

Synonyms TOPI

Category DNA Damage

Background DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. The antitumor agent camptothecin (CPT) reversibly stabilizes the covalent enzyme-DNA intermediate by inhibiting DNA religation. When a replication fork collides with a DNA Top1 cleavage complex, the covalently bound enzyme must be removed from the DNA 3' end before recombination-dependent replication restart. The tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae. In the budding yeast S. cerevisiae, DNA topoisomerases I and II can functionally substitute for each other in removing positive and negative DNA supercoils.

包装清单:

产品编号产品名称包装

LM2053 Topoisomerase I Rabbit Monoclonal Antibody 50μl

LM050 Western一抗稀释液 50ml

- 说明书 1份

保存条件:
       Topoisomerase I Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
       如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
       回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
       提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得ZJ的检测效果，请考虑使用上述检测专用的一抗稀释液。
       本产品仅限于专业人员的科学研究用，不得用于临床诊断或ZL，不得用于食品或药品，不得存放于普通住宅内。
       为了您的安全和健康，请穿实验服并戴一次性手套操作。

温馨提示：不可用于临床ZL。

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产品编号	产品名称	产品包装	产品价格
LM2053	Topoisomerase I Rabbit Monoclonal Antibody	50μl	618.00元

来源	用途	交叉反应性	分子量
Rabbit	WB, IF, IHC, ICC, FC	H, M	91.0kDa

WB	IP	IF	IHC	ICC	FC	ELISA	ChIP
1:1,000-1:5,000	-	1:50-1:500	1:50-1:200	1:50-1:500	1:50-1:100	-	-

About this Antibody
Name	Topoisomerase I Rabbit Monoclonal Antibody
Category	Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype	IgG
Purification	Affinity purification
About the Immunogen
Immunogen	Recombinant protein
Gene ID	7150(Human); 21969(Mouse)
SwissProt	P11387(Human); Q04750(Mouse)
Synonyms	TOPI
Category	DNA Damage
Background	DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. The antitumor agent camptothecin (CPT) reversibly stabilizes the covalent enzyme-DNA intermediate by inhibiting DNA religation. When a replication fork collides with a DNA Top1 cleavage complex, the covalently bound enzyme must be removed from the DNA 3' end before recombination-dependent replication restart. The tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae. In the budding yeast S. cerevisiae, DNA topoisomerases I and II can functionally substitute for each other in removing positive and negative DNA supercoils.

产品编号	产品名称	包装
LM2053	Topoisomerase I Rabbit Monoclonal Antibody	50μl
LM050	Western一抗稀释液	50ml
-	说明书	1份