Category | Primary antibodies |
Product name | MMP9 antibody |
Catalog Number | orb11064 |
Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, IF/ICC, P-ELISA |
Dilution range | P-ELISA: 1:500-1000, IHC-P: 1:400-800, IF/ICC: 1:100-500 |
Concentration | - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml |
Reacts with | Human, Mouse, Rat |
UniProt ID | P14780 (Human); P41245 (Mouse) |
NCBI | NP_004985.21; NM_004994.21 |
Entrez | 4318 |
Conjugation | Unconjugated |
Immunogen | KLH conjugated synthetic peptide derived between 621-697 amino acids of human MMP9 |
Purity | Affinity purified by Protein A |
MW | 78 kDa |
Isotype | IgG |
Form | Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol |
IHC notes | Formalin-fixed paraffin-embedded tissue sections were subjected to antigen retrieval by treatment with 0.01 M Tris-HCl (pH 9.0), followed by incubation with primary MMP9 Antibody (Biorbyt; diluted 1:100) |
Application notes | Western Blot: Load 10 ug protein and block with 5% milk, MMP9 antibody is diluted 1:1000 This antibody can be used for WB, the correct bands. Predicted weight: 64/82/92kDa Tested weight: 64/82/92kDa |
References | Role of fibulin-5 in metastatic organ colonization. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |