Cat. No. 202 003 | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications | WB: 1 : 1000 up to 1 : 10000 (AP staining) suitable for Dot Blot blot IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P/FFPE: not tested yet ELISA: yes suitable for sandwich-ELISA quantification |
Immunogen | N6-methyladenosine fused to BSA. |
Reactivity | Reacts with: human, rat, mouse, eukaryotes, prokaryotes. Other species not tested yet. |
Specificity | Specific for N6-methyladenosine (m6A). |
Remarks | IP: Extracts from eukaryotic and prokaryotic cells. Standard protocol for the IP of nuclear extracts is provided with the product. For the isolation of m6A modified RNA from total or mRNA preparations the protocol according to Dominissini et al. (2013) is recommended. |