active+pro Caspase 3 Rabbit Monoclonal Antibody_详细资料

产品信息

保存条件： -20℃保存供应商：上海联迈生物工程有限公司保质期：一年英文名： active+pro Caspase 3 Rabbit Monoclonal Antibody 数量：大量规格： 50μl

active+pro Caspase 3 Rabbit Monoclonal Antibody

产品编号产品名称产品包装产品价格

LM1675 active+pro Caspase 3 Rabbit Monoclonal Antibody 50μl 618.00元

来源用途交叉反应性分子量

Rabbit WB, IP, IHC H 35.0kDa

       WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
       H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
       配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
       建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB IP IF IHC ICC FC ELISA ChIP

1:1,000-1:2,000 1:10-1:50 - 1:50-1:200 - - - -

抗体详细信息如下:

About this Antibody

Name active+pro Caspase 3 Rabbit Monoclonal Antibody

Category Rabbit Monoclonal Antibody (RabMAb); Primary antibody

Isotype IgG

Purification Affinity purification

About the Immunogen

Immunogen Recombinant protein

Gene ID 836(Human)

SwissProt P42574(Human)

Synonyms CPP32; CPP32B; SCA-1

Category Apoptosis

Background Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

包装清单:

产品编号产品名称包装

LM1675 active+pro Caspase 3 Rabbit Monoclonal Antibody 50μl

LM050 Western一抗稀释液 50ml

- 说明书 1份

保存条件:
       active+pro Caspase 3 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
       如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
       回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
       提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得ZJ的检测效果，请考虑使用上述检测专用的一抗稀释液。
       本产品仅限于专业人员的科学研究用，不得用于临床诊断或ZL，不得用于食品或药品，不得存放于普通住宅内。
       为了您的安全和健康，请穿实验服并戴一次性手套操作。

温馨提示：不可用于临床ZL。

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产品编号	产品名称	产品包装	产品价格
LM1675	active+pro Caspase 3 Rabbit Monoclonal Antibody	50μl	618.00元

来源	用途	交叉反应性	分子量
Rabbit	WB, IP, IHC	H	35.0kDa

WB	IP	IF	IHC	ICC	FC	ELISA	ChIP
1:1,000-1:2,000	1:10-1:50	-	1:50-1:200	-	-	-	-

About this Antibody
Name	active+pro Caspase 3 Rabbit Monoclonal Antibody
Category	Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype	IgG
Purification	Affinity purification
About the Immunogen
Immunogen	Recombinant protein
Gene ID	836(Human)
SwissProt	P42574(Human)
Synonyms	CPP32; CPP32B; SCA-1
Category	Apoptosis
Background	Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

产品编号	产品名称	包装
LM1675	active+pro Caspase 3 Rabbit Monoclonal Antibody	50μl
LM050	Western一抗稀释液	50ml
-	说明书	1份