保存条件:
-20℃保存
供应商:
上海联迈生物工程有限公司
保质期:
一年
英文名:
PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody
数量:
大量
规格:
50μl
PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody 产品编号 | 产品名称 | 产品包装 | 产品价格 |
LM1114 | PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody | 50μl | 618.00元 |
来源 | 用途 | 交叉反应性 | 分子量 |
Rabbit | WB, IP, IF, IHC, ICC | H, M, R | 54.0kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整): WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
1:1000-1:10000 | 1:50-1:200 | 1:50-1:200 | 1:200-1:500 | 1:50-1:200 | - | - | - |
抗体详细信息如下: About this Antibody |
Name | PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody |
Category | Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
Isotype | IgG |
Purification | Affinity purification |
About the Immunogen |
Immunogen | Recombinant protein |
Gene ID | 8503(Human); 18710(Mouse); 60664(Rat) |
SwissProt | Q92569(Human); Q64143(Mouse); Q63789(Rat) |
Synonyms | p55; p55PIK; p55-GAMMA |
Category | PI3K/Akt/mTOR |
Background | Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3’ position. PI 3-kinase p55γ (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55γ protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55γ mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55 γ have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55 γ interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors. |
包装清单: 产品编号 | 产品名称 | 包装 |
LM1114 | PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody | 50μl |
LM050 | Western一抗稀释液 | 50ml |
- | 说明书 | 1份 |
保存条件:
PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得ZJ的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或ZL,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
温馨提示:不可用于临床ZL。