About this Antibody |
Name | Recombinant Murine MCP-5/CCL12 (Recombinant Murine Monocyte Chemotactic Protein-5/CCL12; rMuMCP-5/CCL12);Recombinant Mouse MCP-5/CCL12 ; 重组小鼠单核细胞趋化蛋白5/CCL12 |
Synonyms | MCP-5; C-C motif chemokine 12; chemokine (C-C motif) ligand 12; MCP-5; Scya12 |
Purity | >95% by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50ng/ml. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
Endotoxin | Less than 1EU/μg of rMuMCP-5/CCL12 as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Chemokines |
Background | Murine CCL12 is belonging to the CC chemokine family. It shows 66% amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2. |
Amino Acid Sequence | GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |