人类成纤维细胞CNLMG-B5538SKIN细胞,简称:
产品介绍:
规格数量:5*10^5
运输条件:复苏细胞常温运输,冻存细胞干冰运输
产品品Pai:国产/进口
研究范围:仅供科研使用,切勿用于其它用途
细胞特征:每两到三天进行换液。细胞应当在融合前传代
细胞株状态:活性好、存活率高
随着细胞生物学尤其是干细胞研究的快速发展,在细胞ZL领域已经进行了大量的探索,取得了一系列的令人振奋的成果,且部分基础研究成果已经在心血管系统疾病、神经系统疾病、肌肉骨骼祥光疾病、糖尿病等。人类成纤维细胞CNLMG-B5538SKIN细胞
养细胞注意事项:
1.观察细胞形态。每天必须观察细胞膜,看是否清楚,细胞的死亡往往是从细胞膜的破裂开始的,早期的表现就是某一点细胞膜出现欠完整或者毛发影。
2.细胞往往会有“抱团”现象,抱团生长或死亡,个人认为应该勤换液或传代,尽量避免细胞“抱团”。人类成纤维细胞CNLMG-B5538SKIN细胞
3.细胞生长迅速,强烈建议用较大的培养瓶养,用小瓶养的活,换液不及时,很容易死亡。
4.细胞的生长速度一般为2天左右就需要传代,并且一般为一传三,或一传四。
4.细胞培养液:1640培养液,一般1L加2g碳酸氢钠,并加双抗,胎牛血清浓度为12%,Z低不低于10%,HL60细胞对胎牛血清高度依赖。培养液PH应调为7.20-7.40之间。
5.防止细胞污染。HL60细胞生长迅速,较少发生污染,但常规措施应该做好。比如无菌操作,酒精消毒等,培养瓶口建议过火,包括瓶盖,应过火4秒左右。
6.冻存。-20一小时,-80过一晚,液氮长期保存。强烈建议液氮长期保存,尽量不要再-80长期保存,死亡率很高。
7.细胞复苏:先调节水浴箱温度为42℃,并提前在试管内放置10倍培养液,使培养液温度与试管一致。1分钟内融化细胞(禁止摇晃冻存管),然后移取至试管内,动作一定要轻,从试管管底开始慢慢上提移液管,使细胞在培养液内分布均匀,离心,洗2次。
欢迎科研老师、代理采购前来咨询订购,本页关键词:人类成纤维细胞CNLMG-B5538SKIN细胞
786-258 FOCUS™ Bacterial Proteome 蛋白质组学级细菌蛋白提取 50assays
786-259 FOCUS™ Plant Proteome蛋白质组学级植物蛋白提取 50assays
786-282 Calmodulin Resin钙调素结合树脂 10ml
786-309 PRT-35™ Detergent Concentrate环保型实验室去污剂清洗剂 1L
PW017 辣根过氧化氢酶J-WB显色试剂盒 5membranes
PW023 超敏型辣根过氧化氢酶J-WB显色试剂盒 5membranes
PW033 INT/BCIP碱性磷酸酶显色试剂(棕红色) 5membranes
786-226 Agarose Sealing Solution 2D电泳Agarose 封闭液 50ml
786-230 FOCUS™ Protein Reduction Kit蛋白质还原试剂盒 100preps
786-231 FOCUS™ Protein Reduction- Alkylation Kit蛋白质还原-烷基化试剂盒 100preps
786-232 FOCUS™ Protein Alkylation Kit蛋白质烷基化试剂盒 100preps
786-241 InGel™蛋白质胶内消化 100preps
786-246 FOCUS™ Mammalian Proteome蛋白质组学级动物蛋白提取 50assays
PW038 EZ-Buffers P 5xBLOK Casein in TBS缓冲液 500ml
PW041 无蛋白封闭液 1% 封闭试剂in 1XPBS 500ml
786-168 Fraction-FOCUS™质谱级蛋白分部提取试剂盒 10preps
786-170 Spin-OUT™ GT-600, Micro离心式去盐柱 10columns
786-171 Spin-OUT™ GT-600, Medi离心式去盐柱 10columns
786-181 Tissue PE LB™组织蛋白质裂解液 500ml
786-172 Spin-OUT™ GT-1200, Micro离心式去盐柱 10columns
786-173 Spin-OUT™ GT-1200, Medi离心式去盐柱 10columns
786-174 Spin-OUT™ PCR-20离心式去盐柱 10columns
786-185 Protein FolJ-WseTM Kit蛋白复性试剂盒 80preps
786-175 Spin-OUT™ PCR-32离心式去盐柱 10columns
786-186 FolJ-WseTM I蛋白复性液 I 100ml
786-187 FolJ-WseTM II蛋白复性液 II 100ml
786-188 FolJ-WseTM III蛋白复性液 III 100ml
786-189 FolJ-WseTM IV蛋白复性液 IV 5x1.5ml
786-189D FolJ-WseTM Dilution Buffer蛋白复性液稀释液 50ml
786-190P NAP-BLOCKER in PBS [2X] (no-animal resource blocker)缓冲液 500ml
786-191 FolJ-WseTM V蛋白复性液 V 100ml
786-199 Protein Stains T Silver Destainer银染去银试剂 25minigels
786-21 Protein dotMETRIC™ Kit -----For Rapid Estimation of Protein Concentration微量蛋白浓度确定试剂盒 300assays
786-225 Total Protein Extraction (TPE™)通用总蛋白质提取试剂盒 50assays
786-001 G-CAPSULE™ 电洗脱装置 1盒
786-004 G-CAPSULE™ weight 1个
786-011 BLOT-QuickBlocker缓冲液 175g
786-018 FOCUS™ Global Fractionation膜蛋白分级分离和胞质蛋白提取试剂盒 50assays
786-190 NAP-BLOCKER [2X] (no-animal resource blocker)缓冲液 500ml
786-190T NAP-BLOCKERin TBS [2X] (no-animal resource blocker)缓冲液 500ml
786-020 SPN™ Protein Assays快速GX离心柱式蛋白浓度定量 50assays
786-024-15k Tube-O-Reactor™ (Micro), 15kJ-W MWCO蛋白质交链装置 5reactions
786-024-4k Tube-O-Reactor™ (Micro), 4kJ-W MWCO蛋白质交链装置 5reactions
786-028 Protease Assay Kit蛋白酶活性分析 50assays
786-057 ALLN 10mg
786-024-8k Tube-O-Reactor™ (Micro), 8kJ-W MWCO蛋白质交链装置 5reactions
786-027-15k Tube-O-Reactor™ (Medi), 15kJ-W MWCO蛋白质交链装置 5reactions
786-027-4k Tube-O-Reactor™ (Medi), 4kJ-W MWCO蛋白质交链装置 5reactions
786-058 HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (RhoJ-Wmine)蛋白罗丹明标记试剂盒 5reactions
786-027-8k Tube-O-Reactor™(Medi) 8kJ-W MWCO蛋白质交链装置 5reactions
786-059 HOOK™ Dye Labeling Kit (FITC)蛋白FITC荧光素标记试剂盒 5reactions
786-065 Activated Agarose (Carboxy/Sulfhydryl Reactive)巯基或羧基活化型Agarose 10ml resin
786-066 Activated-NH2- Agarose (Amine Reactive)NH2活化型Agarose 10ml resin
786-067 HOOK™ Peptide Coupling Kit (Amine Reactive)多肽氨基偶联试剂盒 5reactions
786-068 Peptide Coupling Kit (Amine reactive) with one Quant™ BSA 5reactions
786-070 Peptide Coupling Kit (Amine reactive) with one QuantTM HyperCarrierTM 5reactions
786-069 Peptide Coupling Kit (Amine reactive) with one QuantTM KLH 5reactions
786-091 OneQuant™ KLH (Immunological Grade)多管式载体血蓝蛋白 8x2mg
786-096 (HyperCarrier™Immunological Grade)阳性基团取代型BSA 10mg
786-09AP-R43 picoLUCENT™PLUS AP with AP-goat α-mouse antibody皮克级蛋白印迹膜检测 1500cm2
786-09AP-R44 picoLUCENT™ PLUS AP with AP-goat α-rabbit antibody皮克级蛋白印迹膜检测 1500cm2
786-09AP-R45 picoLUCENT™PLUS AP with AP-goat α-rat antibody皮克级蛋白印迹膜检测 1500cm2
786-09-R38 picoLUCENT™ PLUS HRP with HRP-goat αmouse antibody皮克级蛋白印迹膜检测 1000cm2
786-09-R39 picoLUCENT™PLUS HRP with HRP-goat α-rabbit antibody皮克级蛋白印迹膜检测 1000cm2
786-09-R40 picoLUCENT™ PLUS HRP with HRP-goat α-rat antibody皮克级蛋白印迹膜检测 1000cm2
786-110 femtoELISA™-HRP Kit飞克级(TMB)ELISA检测 1000assays
786-112 FemtoELISA™-AP Kit飞克级(TMB)ELISA检测 1000assays
786-120 UPPA-PROTEIN-Concentrate™通用型蛋白沉淀式浓缩试剂盒 1盒
786-123 PAGE-Perfect™PAGE胶样品清洁试剂 50assays
786-124 Perfect-FOCUS™2D电泳样品清洁试剂盒 50assays
786-137 Protease Screening Kit蛋白酶筛选 50assays
786-313 HOOK™ HRP PLUS Labeling kit蛋白HRP标记试剂盒 5reactions
786-315 HOOK™ AP SULFO Labeling kit蛋白AP标记试剂盒 5reactions
786-125 OrgoSol-PROTEIN-Concentrate™活性保持型蛋白沉淀式浓缩试剂盒 1盒
786-126 Column-PROTEIN-Concentrate™柱式微量蛋白浓缩试剂盒 1盒
786-127 OrgoSol DetergentOUT™溶液用去污剂去除试剂 10ml
786-140RF GELPLATE-clean™胶板清洗剂 2x1L
786-145C Micro Dialysis Cups微量透析杯 12cups
786-14 Tube-O-Array™透析平衡装置 1kit
786-151 DetergentOUT™ SDS-300一步式SDS去除柱 10columns
786-152 DetergentOUT™ DTG-100一步式去污剂去除柱 10column
786-161 Femto TBST™ [10] Wash Buffers增强型洗涤液 250ml
786-162 Femto PBST™ [10] Wash Buffers增强型洗涤液 250ml
786-316 HOOK™ Cell Surface Protein Isolation kit细胞膜表面蛋白分离试剂盒 5expts
786-320 Fluoro™ Protease Assay荧光定量法蛋白酶活性分析 1000assays
786-321 Resorufin-Casein Protease Substrate蛋白酶活性分析底物 4mg
786-322 FITC-Casein Protease Substrate蛋白酶活性荧光分析底物 1mg
786-360 FOCUS™ Insect Proteome蛋白质组学级昆虫蛋白提取 50assays
786-386 femtoCHROMO™-HRP with HRP labeled goat α-mouse antibodyHRP型飞克级蛋白印迹膜检测 4000cm2
786-387 femtoCHROMO™-HRP with HRP labeled goat α-rabbit antibodyHRP型飞克级蛋白印迹膜检测 4000cm2
BSP007 Protein Stains F新蛋白质考马斯亮蓝染色试剂盒 10minigels
786-390 Streptavidin Resin 5ml
786-411 Insect PE LB™昆虫细胞裂解液 250ml
786-453 Phosphatase Assay kit磷酸酶活性分析 1000assays
786-501 2D-Xtract™2D电泳样品溶解液 50ml
786-610 Tube-O-DIALYZER™,Micro(up to 250ul)管式透析器 20tubes
786-611 Tube-O-DIALYZER™,Micro(up to 250ul)管式透析器 20tubes
786-615 Tube-O-DIALYZER™ Medi(up to 2.5ml)管式透析器 20tubes
786-612 Tube-O-DIALYZER™ .Micro(up to 250ul)管式透析器 20tubes
786-613 Tube-O-DIALYZER™,Micro(up to 250ul)管式透析器 20tubes
786-616 Tube-O-DIALYZER™ Medi(up to 2.5ml)管式透析器 20tubes
786-617 Tube-O-DIALYZER™,Medi(up to 2.5ml)管式透析器 20tubes
786-618 Tube-O-DIALYZER™,Medi(up to 2.5ml)管式透析器 20tubes
786-619 Tube-O-DIALYZER™ Medi(up to 2.5ml)管式透析器 20tubes
AD439-1g APO 8 1g
AD439-5g APO 8 5g
AD441-1g APO 10 1g
AD441-5g APO 10 5g
BE667-100ml EZ-Block B in 1xTBS Solution印迹膜封闭液 100ml
BE667-500ml EZ-Block B in 1xTBS Solution印迹膜封闭液 500ml
BE671-100ml EZ-Block A IN PBS Solution印迹膜封闭液 100ml
BE671-500ml EZ-Block A IN PBS Solution印迹膜封闭液 500ml
BGI212 Bolton-Hunter Reagent 100mg
BKC-04 Optimizer Buffer™-I蛋白质交链液 2x25ml
BKC-05 Optimizer Buffer™ -II蛋白质交链液 2x25ml
BKC-06 Optimizer Buffer™ - III蛋白质交链液 2x25ml
BKC-07 Optimizer Buffer™ - IV蛋白质交链液 2x25ml
BKC-08 Optimizer Buffer™ - V蛋白质交链液 2x25ml
BKC-09 Optimizer Buffer™ - VI蛋白质交链液 2x25ml
BS380 Protease Inhibitor Cocktall (For Bacterial Cell, with EDTA)蛋白酶YZ剂 100 1ml
BS381 Protease Inhibitor Cocktall (For Bacterial Cell, with EDTA)蛋白酶YZ剂 100 1ml
BS382 Protease Inhibitor Cocktall (For Fungal/Yeast Cell)蛋白酶YZ剂 100 1ml
BS384 Protease Inhibitor Cocktall (For Plant Cell)蛋白酶YZ剂 100 1ml
BS386 Protease Inhibitor Cocktall (For Mammalian Cell)蛋白酶YZ剂 100 1ml
BS387 Protease Inhibitor Cocktall (For Mammalian Cell)蛋白酶YZ剂 100 1ml
BS392-MS Western Blot Kit( With HRP Conjugated Mouse SeconJ-Wry Antibody)Western Blot试剂盒 (NC Membrane) 50-100samples
BS392-RB Western Blot Kit( With HRP Conjugated Rabbit SeconJ-Wry Antibody)Western Blot试剂盒 (NC Membrane) 50-100samples
BS392-RT Western Blot Kit( With HRP Conjugated Rat SeconJ-Wry Antibody)Western Blot试剂盒 (NC Membrane) 50-100samples
BS393-MS Western Blot Kit( With HRP Conjugated Mouse SeconJ-Wry Antibody)Western Blot试剂盒 (PVDF Membrane) 50-100samples
BS393-RB Western Blot Kit( With HRP Conjugated Rabbit SeconJ-Wry Antibody)Western Blot试剂盒 (PVDF Membrane) 50-100samples
BS393-RT Western Blot Kit( With HRP Conjugated Rat SeconJ-Wry Antibody)Western Blot试剂盒 (PVDF Membrane) 50-100samples
BS4447-250ml Brij 56 250ml
BS4447-50ml Brij 56 50ml
BS4448-250ml Brij 58 250ml
BS4448-50ml Brij 58 50ml
BS589 Mammalian protein Extraction Kit 50assays
BS596 Bacterial Protein Extraction Kit细菌总蛋白提取试剂盒 for 1 liter culture
BS597 Bacterial Protein Extraction Kit细菌总蛋白提取试剂盒 for 5 liter culture
BS686 Inclusion Body Protein Extaction Kit包涵体蛋白抽提试剂盒 for 10g bacteria
BS687 Inclusion Body Protein Extaction Kit包涵体蛋白抽提试剂盒 for 30g bacteria
BS688 Immunoprecipitation Kit (With Protein A Agarose)免疫沉淀试剂盒 20reactions
BS689 Immunoprecipitation Kit (With Protein A/G Agarose)免疫沉淀试剂盒 20reactions
BS690 Immunoprecipitation Kit (With Pprotein A sepharose)免疫沉淀试剂盒 20reactions
BSP093-4 Heparin Resin 5ml(预装重力柱) 10个
BSP095-4 Protein A Resin 5ml(预装重力柱) 10个
CA0220 C10 E9 1g(10ml)
BS694 Protein A/G Plus-SefinoseTM Resin (Settled Resin) 1ml
BSB216 Sulfo NHS SS Biotin 50mg
BSB220 Biotin LC-Hydrazide 50mg
BSB222 Biotin BMCC 50mg
BSP001 Nuclear and Cytoplasmic Protein Extraction Kit细胞核蛋白/浆蛋白抽提试剂盒 50assays
BSP002 膜蛋白,核蛋白和胞质蛋白抽提试剂盒 50次
BSP003 Tissue or Cell Total Protein Extraction Kit全蛋白提取试剂盒 50assays
BSP004 一步法植物活性蛋白质提取试剂盒 20次
BSP005 Membrane and Cytoplasmic Protein Extraction kit膜蛋白和胞质蛋白提取试剂盒 50assays
BSP006 一步法动物组织活性蛋白提取试剂盒 20次
D0098-1g Octyl- β-D-thioglucoside 1g
BSP009 Nucleoprotein Protein Extraction Kit细胞核蛋白质提取试剂盒 50assays
BSP011 TCA Protein Precipitation Kit三氯醋酸浓缩试剂盒 50preps
BSP012 Universal Protein Sediment Reagent通用型蛋白沉淀试剂 100preps
BSP013 Yeast Total Protein Extraction Kit酵母蛋白提取试剂盒 50assays
BSP015 Protein Stains G新型快速考马斯亮蓝染色试剂盒 10minigels
BSP017 Protein Stains I可逆型铜染试剂盒 10minigels
BSP019 Protein Stains J可逆型锌染试剂盒 10minigels
BSP020 一步法昆虫细胞活性蛋白提取试剂盒 20次
BSP021 Protein Stains K质谱用快速银染试剂盒 10minigels
BSP022 一步法动物细胞活性蛋白提取试剂盒 20次
BSP023 一步法细菌活性蛋白提取试剂盒 20次
BSP024 超敏可逆印迹膜染色试剂盒I 10次
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