EB病毒转化的人B淋巴细胞KM9401细胞,简称:
产品介绍:
规格数量:5*10^5
运输条件:复苏细胞常温运输,冻存细胞干冰运输
产品品Pai:国产/进口
研究范围:仅供科研使用,切勿用于其它用途
细胞特征:每两到三天进行换液。细胞应当在融合前传代
细胞株状态:活性好、存活率高
随着细胞生物学尤其是干细胞研究的快速发展,在细胞ZL领域已经进行了大量的探索,取得了一系列的令人振奋的成果,且部分基础研究成果已经在心血管系统疾病、神经系统疾病、肌肉骨骼祥光疾病、糖尿病等。EB病毒转化的人B淋巴细胞KM9401细胞
养细胞注意事项:
1.观察细胞形态。每天必须观察细胞膜,看是否清楚,细胞的死亡往往是从细胞膜的破裂开始的,早期的表现就是某一点细胞膜出现欠完整或者毛发影。
2.细胞往往会有“抱团”现象,抱团生长或死亡,个人认为应该勤换液或传代,尽量避免细胞“抱团”。EB病毒转化的人B淋巴细胞KM9401细胞
3.细胞生长迅速,强烈建议用较大的培养瓶养,用小瓶养的活,换液不及时,很容易死亡。
4.细胞的生长速度一般为2天左右就需要传代,并且一般为一传三,或一传四。
4.细胞培养液:1640培养液,一般1L加2g碳酸氢钠,并加双抗,胎牛血清浓度为12%,Z低不低于10%,HL60细胞对胎牛血清高度依赖。培养液PH应调为7.20-7.40之间。
5.防止细胞污染。HL60细胞生长迅速,较少发生污染,但常规措施应该做好。比如无菌操作,酒精消毒等,培养瓶口建议过火,包括瓶盖,应过火4秒左右。
6.冻存。-20一小时,-80过一晚,液氮长期保存。强烈建议液氮长期保存,尽量不要再-80长期保存,死亡率很高。
7.细胞复苏:先调节水浴箱温度为42℃,并提前在试管内放置10倍培养液,使培养液温度与试管一致。1分钟内融化细胞(禁止摇晃冻存管),然后移取至试管内,动作一定要轻,从试管管底开始慢慢上提移液管,使细胞在培养液内分布均匀,离心,洗2次。
欢迎科研老师、代理采购前来咨询订购,本页关键词:EB病毒转化的人B淋巴细胞KM9401细胞
G6147-25g Guinea green B 基尼绿B [4680-78-8] 25g
HB0494-1g Hematin 血红素 [15489-90-4] 1g
H2521-100g Hemin Bovine 氯化血红素 [16009-13-5] 100g
G6222-250g Guaiacol glyceryl ether 愈创木酚甘油醚 [93-14-1] 250g
GB0491-250g Guanidine carbonate 碳酸胍 [593-85-1] 250g
GB0491-1kg Guanidine carbonate 碳酸胍 [593-85-1] 1kg
GB0243-100g Guanidine hydrochloride 盐酸胍 [50-01-1] 100g
GB0243-500g Guanidine hydrochloride 盐酸胍 [50-01-1] 500g
GB0242-100g Guanidine hydrochloride 盐酸胍 [50-01-1] 100g
GB0242-500g Guanidine hydrochloride 盐酸胍 [50-01-1] 500g
G0287-25g Guanidine hydrochloride 盐酸胍 [50-01-1] 25g
G0287-100g Guanidine hydrochloride 盐酸胍 [50-01-1] 100g
G0287-500g Guanidine hydrochloride 盐酸胍 [50-01-1] 500g
GE424-100g Guanidine hydrochloride, low ASH 盐酸胍 [50-01-1] 100g
GE424-500g Guanidine hydrochloride, low ASH 盐酸胍 [50-01-1] 500g
GE424-2.5kg Guanidine hydrochloride, low ASH 盐酸胍 [50-01-1] 2.5kg
G3057-100g Guanidine nitrate 硝酸胍 [506-93-4] 100g
GDR0244-100g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 100g
GDR0244-500g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 500g
GB0244-100g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 100g
GB0244-500g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 500g
G0380-50g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 50g
G0380-250g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 250g
G0380-500g Guanidine thiocyanate 硫氰酸胍 [593-84-0] 500g
GD0246-25g Guanine 鸟嘌呤 [73-40-5] 25g
GD0246-100g Guanine 鸟嘌呤 [73-40-5] 100g
GD0250-25g Guanosine 鸟苷 [118-00-3] 25g
GD0250-100g Guanosine 鸟苷 [118-00-3] 100g
GB0492-100mg GDP disodium salt; Guanosine-5’-diphosphate, disodium salt 5’-二磷酸鸟苷二钠盐 [7415-69-2] 100mg
GB0492-500mg GDP disodium salt; Guanosine-5’-diphosphate, disodium salt 5’-二磷酸鸟苷二钠盐 [7415-69-2] 500mg
GB0493-5g GDP disodium salt hydrate;Guanosine-5’-monophosphate, disodium salt hydrate 5’-单磷酸鸟苷二钠盐 [5550-12-9] 5g
GD0250T-100mg GTP trisodium salt hydrate; Guanosine-5’-triphosphate, trisodium salt hydrate 5’-三磷酸鸟苷三钠 [36051-31-7] 100mg
GD0250T-500mg GTP trisodium salt hydrate; Guanosine-5’-triphosphate, trisodium salt hydrate 5’-三磷酸鸟苷三钠 [36051-31-7] 500mg
GB0332-0.25ml GTP 100 mM solution; Guanosine-5’-triphosphate, 100 mM solution 5’-三磷酸鸟苷三钠溶液 [36051-31-7] 0.25ml
G2131-250g Gum copa 柯柏胶 (硬树脂) / 250g
H0701-10g Hematoxylin 苏木色素 [517-28-2] 10g
H0701-50g Hematoxylin 苏木色素 [517-28-2] 50g
HB0267-150KU Hemicellulase 半纤维素酶 / 150KU
HLS002544-250mg Histone 组蛋白 / 250mg
HLS002546-1g Histone 组蛋白 / 1g
HLS002402-5g Hemoglobin 血红蛋白 / 5g
HLS002402-25g Hemoglobin 血红蛋白 / 25g
HLS002402-100g Hemoglobin 血红蛋白 / 100g
HB3251-1g Heparin sodium from Porcine Intestinal 肝素钠盐 [9041-08-1] 1g
HB0495-25g HEPBS N-(2-羟乙基)哌嗪-N′-4-丁磺酸 [161308-36-7] 25g
HB0495-100g HEPBS N-(2-羟乙基)哌嗪-N′-4-丁磺酸 [161308-36-7] 100g
HB0264-50g HEPES, free acid N-(2-羟乙基)哌嗪- N′-2磺酸 [7365-45-9] 50g
HB0264-250g HEPES, free acid N-(2-羟乙基)哌嗪- N′-2磺酸 [7365-45-9] 250g
HB0264-1kg HEPES, free acid N-(2-羟乙基)哌嗪- N′-2磺酸 [7365-45-9] 1kg
H0511-50g HEPES, free acid N-(2-羟乙基)哌嗪- N′-2磺酸 [7365-45-9] 50g
H0511-250g HEPES, free acid N-(2-羟乙基)哌嗪- N′-2磺酸 [7365-45-9] 250g
H0511-1kg HEPES, free acid N-(2-羟乙基)哌嗪- N′-2磺酸 [7365-45-9] 1kg
HB0265-50g HEPES, sodium salt N-(2-羟乙基)哌嗪- N′-(2-乙磺酸)钠盐 [75277-39-3] 50g
HB0265-250g HEPES, sodium salt N-(2-羟乙基)哌嗪- N′-(2-乙磺酸)钠盐 [75277-39-3] 250g
HB0265-1kg HEPES, sodium salt N-(2-羟乙基)哌嗪- N′-(2-乙磺酸)钠盐 [75277-39-3] 1kg
H4064-50g D-Histidine D-组氨酸 [351-50-8] 50g
HH115-500ml Histochoice® Dermatology fixative 组织切片固定剂 / 500ml
H0485-25g HEPES, sodium salt N-(2-羟乙基)哌嗪- N′-(2-乙磺酸)钠盐 [75277-39-3] 25g
H0485-100g HEPES, sodium salt N-(2-羟乙基)哌嗪- N′-(2-乙磺酸)钠盐 [75277-39-3] 100g
H0485-500g HEPES, sodium salt N-(2-羟乙基)哌嗪- N′-(2-乙磺酸)钠盐 [75277-39-3] 500g
HDJ699-25g HEPPS N-2-羟乙基哌嗪-N’-3-丙磺酸 [16052-06-5] 25g
HDJ699-100g HEPPS N-2-羟乙基哌嗪-N’-3-丙磺酸 [16052-06-5] 100g
HJ588-25g HEPPS N-2-羟乙基哌嗪-N’-3-丙磺酸 [16052-06-5] 25g
HJ588-100g HEPPS N-2-羟乙基哌嗪-N’-3-丙磺酸 [16052-06-5] 100g
HDJ698-25g HEPPSO, free acid N-2-羟乙基哌嗪-N’-2-羟基丙磺酸 [68399-78-0] 25g
HDJ698-100g HEPPSO, free acid N-2-羟乙基哌嗪-N’-2-羟基丙磺酸 [68399-78-0] 100g
HJ587-25g HEPPSO, free acid N-2-羟乙基哌嗪-N’-2-羟基丙磺酸 [68399-78-0] 25g
HJ587-100g HEPPSO, free acid N-2-羟乙基哌嗪-N’-2-羟基丙磺酸 [68399-78-0] 100g
HB0496-25g HEPPSO, sodium salt N-2-羟乙基哌嗪-N’-2-羟基丙磺酸钠盐 [89648-37-3] 25g
HB0496-100g HEPPSO, sodium salt N-2-羟乙基哌嗪-N’-2-羟基丙磺酸钠盐 [89648-37-3] 100g
HB6622-250ml Heptane 正庚烷 [142-82-5] 250ml
H1846-250ml n-Heptanoic acid 正庚酸 [111-14-8] 250ml
H6623-1g n-Heptyl β-D-glucopyranoside 正庚基-beta-D-吡喃葡萄糖苷 [78617-12-6] 1g
H6624-50mg Hesperidin 橙皮甙 [520-26-3] 50mg
H1532-250g Hexachloroethane 六氯乙烷 [67-72-1] 250g
HB0497-250g 1-Hexadecanoic acid 十六酸 [57-10-3] 250g
H1468-1kg 1-Hexadecanol 十六醇 [36653-82-4] 1kg
HB0498-25g Hexadecyl dimethyl benzyl ammonium chloride N-十六烷基-N,N-二甲基苄基氯化铵 [122-18-9] 25g
HB0498-100g Hexadecyl dimethyl benzyl ammonium chloride N-十六烷基-N,N-二甲基苄基氯化铵 [122-18-9] 100g
HB0499-500g Hexamethylenetetramine 六次甲基四胺 [100-97-0] 500g
H6105-100g Hexamethylphosphoramide 六甲基磷酰三胺 [680-31-9] 100g
HB7891-1g Hexammine cobalt(III) chloride 三氯六氨络钴 [10534-89-1] 1g
HB7891-5g Hexammine cobalt(III) chloride 三氯六氨络钴 [10534-89-1] 5g
H1511-500g 1,6-Hexanediamine 1,6-己二胺 [124-09-4] 500g
H1513-100g 1,6-Hexanediol 1,6-己二醇 [629-11-8] 100g
HN1231-5g 1-Hexanesulfonic acid, sodium salt 1-已烷磺酸钠 [2832-45-3] 5g
H4033-5ml n-Hexanol 正己醇 [111-27-3] 5ml
Y46560093-500U Hexokinase (Grade I) 己糖激酶 [9001-51-8] 500U
H7060-500ml Hexylene glycol 己二醇 [107-41-5] 500ml
H6625-1g Hexyl-beta-D-maltoside 正己基--beta-D-麦牙糖苷 [870287-95-9] 1g
H1517-25g Hippuric acid 马尿酸 [495-69-2] 25g
HB0500-1g Histamine 组胺 [51-45-6] 1g
HB0500-5g Histamine 组胺 [51-45-6] 5g
HB0501-5g Histamine dihydrochloride 二盐酸组胺 [56-92-8] 5g
HB0501-25g Histamine dihydrochloride 二盐酸组胺 [56-92-8] 25g
HB0502-5g Histamine diphosphate 二磷酸组胺 [51-74-1] 5g
HB0502-25g Histamine diphosphate 二磷酸组胺 [51-74-1] 25g
H2860-50g DL-Histidine DL-组氨酸 [4998-57-6] 50g
HB0330-100g L-Histidine, hydrochloride L-组氨酸盐酸盐 [645-35-2] 100g
HB0330-500g L-Histidine, hydrochloride L-组氨酸盐酸盐 [645-35-2] 500g
HE806-25g L-Histidine, monohydrochloride, monohydrate L-组氨酸一盐酸盐 一水 [5934-29-2] 25g
HE806-100g L-Histidine, monohydrochloride, monohydrate L-组氨酸一盐酸盐 一水 [5934-29-2] 100g
HH157-100ml Histochoice® mounting media 组织切片清除剂 / 100ml
HLS002375-250mg Histones , dried 组蛋白A / 250mg
HLS002375-1g Histones , dried 组蛋白A / 1g
HLS003010-250mg Histone 组蛋白 / 250mg
HLS003010-1g Histone 组蛋白 / 1g
H2842-25g L-Homoarginine hydrochloride L-高精氨酸盐酸盐 [1483-01-8] 25g
H4059-25g L-Home-phenylalanine L-高苯丙氨酸 [943-73-7] 25g
H714332-1g Hyaluronic acid 透明质酸 from bovine vitreous humor [9004-61-9] 1g
HLS003907-10mg Hyaluronic acid 透明质酸 from bovine vitreous humor [9004-61-9] 10mg
HB0503-25KU HyaluroniJ-Wse 透明质酸酶from bovine vitreous humor [37326-33-3] 25KU
HLS003909-50mg Hyaluronic acid 透明质酸 from bovine vitreous humor [9004-61-9] 50mg
HLS003910-100mg Hyaluronic acid 透明质酸 from bovine vitreous humor [9004-61-9] 100mg
H7076-1g Hyaluronic acid sodium salt 透明质酸钠 [9067-32-7] 1g
HLS002594-50KU HyaluroniJ-Wse, purified 透明质酸酶 from bovine vitreous humor [37326-33-3] 50KU
HLS002594-300KU HyaluroniJ-Wse, purified 透明质酸酶 from bovine vitreous humor [37326-33-3] 300KU
HB0504-100g Hydrazine hydrochloride 盐酸肼 [2644-70-4] 100g
H6628-20mg Hyperoside 金丝桃苷 [482-36-0] 20mg
H6628-100mg Hyperoside 金丝桃苷 [482-36-0] 100mg
IB0532-10g Indigo carmine 靛兰胭脂红 [860-22-0] 10g
I6326-5g Indocyanine green 吲哚菁绿 [3599-32-4] 5g
HB0505-100g Hydrazine sulfate 硫酸肼 [10034-93-2] 100g
H6472-50g 2-Hydrazinobenzoic acid hydrochloride 2-肼基苯甲酸盐酸盐 [52356-01-1] 50g
H6473-50g 4-Hydrazinobenzoic acid hydrochloride 4-肼基苯甲酸盐酸盐 [24589-77-3] 50g
H6626-5g HydrinJ-Wntin hydrate 还原茚三酮二水合物 [5950-69-6] 5g
H2165-100ml Hydrobromic acid 氢溴酸 [10035-10-6] 100ml
HB0506-5g Hydrocortisone 氢化可的松 [50-23-7] 5g
H2166-50ml Hydroiodic acid 氢碘酸 [10034-85-2] 50ml
H1808-100g Hydroquinone 对苯二酚 [123-31-9] 100g
H3063-500g 2-Hydroxyacetophenone 邻羟基苯乙酮 [118-93-4] 500g
H3062-250g 4-Hydroxyacetophenone 对羟基苯乙酮 [99-93-4] 250g
H5097-500g 3-Hydroxybenzaldehyde 3-羟基苯甲醛 [100-83-4] 500g
HB0507-100g 4-Hydroxybenzaldehyde 4-羟基苯甲醛 [123-08-0] 100g
HB0508-100g p-Hydroxybenzoic acid 对羟基苯甲酸 [99-96-7] 100g
H1821-500g m-Hydroxybenzoic acid 间羟基苯甲酸 [99-06-9] 500g
HB0509-25g p-Hydroxybenzoic acid, sodium salt 对羟基苯甲酸钠 [114-63-6] 25g
HB0509-100g p-Hydroxybenzoic acid, sodium salt 对羟基苯甲酸钠 [114-63-6] 100g
H1824-250g p-Hydroxybenzoic acid butyl ester 对尼泊金丁酯 [94-26-8] 250g
H5035-250g p-Hydroxybenzoic acid butyl ester sodium salt 对尼泊金丁酯钠 [36457-20-2] 250g
H1822-250g p-Hydroxybenzoic acid ethyl ester 尼泊金乙酯 [120-47-8] 250g
H3036-250g p-Hydroxybenzoic acid ethyl ester sodium salt 尼泊金乙酯钠 [35285-68-8] 250g
HB0510-100g m-Hydroxybenzoic acid methyl ester 3-羟基苯甲酸甲酯 [19438-10-9] 100g
HB0510-500g m-Hydroxybenzoic acid methyl ester 3-羟基苯甲酸甲酯 [19438-10-9] 500g
HN1186-100g p-Hydroxybenzoic acid methyl ester 尼泊金甲酯 [99-76-3] 100g
H1901-250g p-Hydroxybenzoic acid methyl ester sodium salt 尼泊金甲酯钠 [5026-26-0] 250g
H4039-250g p-Hydroxybenzoic acid propyl este 尼泊金丙酯 [94-13-3] 250g
H5034-250g p-Hydroxybenzoic acid propyl ester sodium salt 尼泊金丙酯钠 [35285-69-9] 250g
HB0511-50g 1-Hydroxybenzotriazole monohydrate 对羟基苯三唑一水 [2592-95-2] 50g
HB0511-250g 1-Hydroxybenzotriazole monohydrate 对羟基苯三唑一水 [2592-95-2] 250g
H6505-25g 4-Hydroxycarbazole 4-羟基咔唑 [52602-39-8] 25g
H5098-25g 3-Hydroxycinnamic acid 间羟基肉桂酸 [14755-02-3] 25g
H6158-250g trans-4-Hydroxycinnamic acid 4-羟基肉桂酸 [501-98-4] 250g
H6259-250g 4-Hydroxycoumarin 4-羟基香豆素 [1076-38-6] 250g
H6484-50g 7-Hydroxycoumarin 7-羟基香豆素 [93-35-6] 50g
HB0512-250g Hydroxyethyl Cellulose 低粘度羟乙基纤维素 [9004-62-0] 250g
HB0512-1kg Hydroxyethyl Cellulose 低粘度羟乙基纤维素 [9004-62-0] 1kg
HB0513-250g Hydroxyethyl Cellulose 中粘度羟乙基纤维素 [9004-62-0] 250g
HB0513-1kg Hydroxyethyl Cellulose 中粘度羟乙基纤维素 [9004-62-0] 1kg
I6554-10g Insect moulting hormone 蜕皮激素 [5289-74-7] 10g
IB0280-1g INT dye 碘硝基四唑紫 [146-68-9] 1g
IB0280-5g INT dye 碘硝基四唑紫 [146-68-9] 5g
HB0514-250g Hydroxyethyl Cellulose 高粘度羟乙基纤维素 [9004-62-0] 250g
HB0514-1kg Hydroxyethyl Cellulose 高粘度羟乙基纤维素 [9004-62-0] 1kg
HK391-100g Hydroxyethyl Cellulose 高粘度羟乙基纤维素 [9004-62-0] 100g
HK391-500g Hydroxyethyl Cellulose 高粘度羟乙基纤维素 [9004-62-0] 500g
H6627-1g Hydroxyectoine 5-羟基-2甲基-1,4,5,6-四氢嘧啶4甲酸 [165542-15-4] 1g
H6627-5g Hydroxyectoine 5-羟基-2甲基-1,4,5,6-四氢嘧啶4甲酸 [165542-15-4] 5g
HB0515-100g Hydroxylamine hydrochloride 盐酸羟胺 [5470-11-1] 100g
HB0515-500g Hydroxylamine hydrochloride 盐酸羟胺 [5470-11-1] 500g
HB0516-100g Hydroxylamine sulfate 硫酸羟胺 [10039-54-0] 100g
HB0516-500g Hydroxylamine sulfate 硫酸羟胺 [10039-54-0] 500g
H6518-25g 3-Hydroxy-methylbenzoic acid 3-羟基-4-甲基苯甲酸 [586-30-1] 25g
H7068-100g 7-Hydroxy-4-methylcoumarin 7-羟基-4-甲基香豆素 [90-33-5] 100g
H6152-250g N-(Hydroxymethyl)phthalimide N-羟甲基邻苯二甲酰亚胺 [118-29-6] 250g
H6836-100g 2-Hydroxyphenylacetic acid 2-羟基苯乙酸 [614-75-5] 100g
H6835-100g 4-Hydroxyphenylacetic acid 4-羟基苯乙酸 [156-38-7] 100g
H5067-50g 2-(4-Hydroxyphenyl) ethanol 对羟基苯乙醇 [501-94-0] 50g
H6519-250g N-Hydroxyphthalimide N-羟基邻苯二甲酰亚胺 [524-38-9] 250g
H6520-25g 3-Hydroxypicolinic acid 3-羟基-2-吡啶甲酸 [874-24-8] 25g
H6521-25g 6-Hydroxypicolinic acid 6-羟基-2-吡啶甲酸 [19621-92-2] 25g
HB0517-25g L-Hydroxyproline 反式-4-羟基-L-脯氨酸 [51-35-4] 25g
HB0517-100g L-Hydroxyproline 反式-4-羟基-L-脯氨酸 [51-35-4] 100g
HK388-1g Hydroxypropyl-β-cyclodextrin 羟丙基-β-环糊精 [128446-35-5] 1g
HK388-5g Hydroxypropyl-β-cyclodextrin 羟丙基-β-环糊精 [128446-35-5] 5g
HB0519-250g Hydroxypropyl cellulose 低粘度羟丙基纤维素 [9004-64-2] 250g
HB0519-1kg Hydroxypropyl cellulose 低粘度羟丙基纤维素 [9004-64-2] 1kg
HB0520-250g Hydroxypropyl cellulose 中粘度羟丙基纤维素 [9004-64-2] 250g
HB0520-1kg Hydroxypropyl cellulose 中粘度羟丙基纤维素 [9004-64-2] 1kg
HB0521-250g Hydroxypropyl methyl cellulose 低粘度羟丙基甲基纤维素 [9004-65-3] 250g
KB0745-100g Kinetin -糠基氨基嘌呤 [525-79-1] 100g
I1795-250g Iron (II,III ) oxide 四氧化三铁 [1317-61-9] 250g
Y46475003-150U Isocitrate dehydrogenase 异柠檬酸脱氢酶 / 150U
HB0521-1kg Hydroxypropyl methyl cellulose 低粘度羟丙基甲基纤维素 [9004-65-3] 1kg
HB0522-250g Hydroxypropyl methyl cellulose 中粘度羟丙基甲基纤维素 [9004-65-3] 250g
HB0522-1kg Hydroxypropyl methyl cellulose 中粘度羟丙基甲基纤维素 [9004-65-3] 1kg
HB0523-250g Hydroxypropyl methyl cellulose 高粘度羟丙基甲基纤维素 [9004-65-3] 250g
HB0523-1kg Hydroxypropyl methyl cellulose 高粘度羟丙基甲基纤维素 [9004-65-3] 1kg
HB0529-250g Hydroxypropyl methyl cellulose 羟丙基甲基纤维素 [9004-65-3] 250g
HB0529-1kg Hydroxypropyl methyl cellulose 羟丙基甲基纤维素 [9004-65-3] 1kg
HK392-10g Hydroxypropyl methyl cellulose 羟丙基甲基纤维素 [9004-65-3] 10g
HK392-100g Hydroxypropyl methyl cellulose 羟丙基甲基纤维素 [9004-65-3] 100g
HB0524-10g 8-Hydroxyquinaldine 8-羟基喹哪啶 [826-81-3] 10g
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