SYTO™ 21 绿色荧光核酸染色染料 - 5 mM Solution in DMSO 货号S7556
SYTO™ 21 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO描述The cell-permeant SYTO 21 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #2 (S-7554) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.规格
Color: | Green |
For Use With (Equipment): | Fluorescence Microscope |
Cell Permeability: | Cell-Permeant |
Sub-Cellular Localization: | Nucleic Acids |
Detection Method: | Fluorescent |
Excitation⁄Emission (nm): | 494⁄517 |
Product Line: | SYTO® |
Product Size: | 250 µL |
Shipping Condition: | Room Temperature |
内容及储存Store in freezer (-5 to -30°C) and protect from light.
Current prices may be obtained from our website or from our Customer Service Department.
Cat. No. | Product name | Unit size |
S34854 | SYTO® 9 green fluorescent nucleic acid stain *5 mM solution in DMSO* | 100 µL |
S7573 | SYTO® 11 green fluorescent nucleic acid stain *5 mM solution in DMSO* | 250 µL |
S7574 | SYTO® 12 green fluorescent nucleic acid stain *4 mM solution in DMSO* | 315 µL |
S7575 | SYTO® 13 green fluorescent nucleic acid stain *5 mM solution in DMSO* | 250 µL |
S7576 | SYTO® 14 green fluorescent nucleic acid stain *5 mM solution in DMSO* | 250 µL |
S7578 | SYTO® 16 green fluorescent nucleic acid stain *1 mM solution in DMSO* | 250 µL |
S7556 | SYTO® 21 green fluorescent nucleic acid stain *5 mM solution in DMSO* | 250 µL |
S7559 | SYTO® 24 green fluorescent nucleic acid stain *5 mM solution in DMSO* | 250 µL |
S34855 | SYTO® BC green fluorescent nucleic acid stain *5 mM solution in DMSO* | 100 µL |
S7572 | SYTO® Green-Fluorescent Nucleic Acid Stain Sampler Kit *SYTO® dyes 11–14, 16, 21, 24 *50 µL each* | 1 kit |
Table 2 Spectral characteristics of SYTO ® green-fluorescent nucleic acid stains
Dye | Cat. No. | Absorption* (nm) | Emission* (nm) | QY† DNA | QY† RNA |
+DNA | +RNA | +DNA | +RNA |
SYTO® 9 | S34854 | 485 | 486 | 498 | 501 | 0.58 | ND |
SYTO® 11 | S7573 | 508 | 510 | 527 | 530 | 0.49 | 0.39 |
SYTO® 12 | S7574 | 499 | 500 | 522 | 519 | 0.09 | 0.13 |
SYTO® 13 | S7575 | 488 | 491 | 509 | 514 | 0.40 | 0.40 |
SYTO® 14 | S7576 | 517 | 521 | 549 | 547 | 0.08 | 0.12 |
SYTO® 16 | S7578 | 488 | 494 | 518 | 525 | 0.65 | 0.24 |
SYTO® 21 | S7556 | 494 | ND | 517 | ND | ~0.5 | ND |
SYTO® 24 | S7559 | 490 | ND | 515 | ND | 0.76 | ND |
SYTO® BC | S34855 | 485 | 487 | 500 | 504 | ND | ND |
* Absorption and fluorescence emission maxima determined in the presence of DNA or RNA using a ratio of ~100 bases of nucleic acid to 1 dye molecule. For SYTO® 11–16 dyes plus DNA the spectra were determined in 10 mM Tris, 1 mM EDTA, 50 mM NaCl, pH 7.4; for SYTO® 11–16 dyes plus RNA the spectra were determined in 25 mM HEPES, pH 7.5; for SYTO® 21 and 24 the spectra were determined in 50 mM Tris, 1 mM EDTA, pH 7.5.
† The fluorescence quantum yield was determined for SYTO® dyes in the presence of DNA or RNA and expressed relative to that determined for fluorescein in buffer at pH 9.0 (assumed to be 0.92 under these conditions).
ND = not determined.
温馨提示:不可用于临床ZL。