产品名称:LC-SPDP 英文全称:(succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate) General description The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.
简介:LC-SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive het-erobifunctional protein crosslinker. The "long chain" LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, compared to the 6.8 angstrom spacer arm of SPDP.
外观:白色干燥粉末 Molecular Weight: 425.5 分子式C18H23O5N3S2 溶解性:DMSO or DMF Spacer Length: 15.7 Å Alternative Name: Succinimidyl-6-(3-[2-pyridyldithiol-propionamido) hexanoate 纯度:98%+ 保存:-20℃ 厂家:西安瑞禧生物科技有限公司