Sirius Red Total Collagen Detection Kit（天狼星红总胶原检测试剂盒 9062）

INTRODUCTION
Sirius red is a unique dye which specifically binds to the [Gly-X-Y]n helical structure on fibrillar collagen (type I to V) and does not discriminate between collagen species and types.  Therefore, this kit is designed to detect the total collagen content in various samples.
Chondrex, Inc. provides a Sirius Red Collagen Detection Kit for various collagen-containing samples such as tissue specimens, cell culture media, and cultured cells.  The total assay working time is less than 30 minutes and 40 samples can be measured in duplicate.  Due to the low level of collagen in cell culture media, additional concentration steps may be necessary. 
For determining levels of collagen from individual species or different types of samples, we recommend our Type I Collagen Detection kits (Catalog # 6012-6016, 6019, 6021) and Type II Collagen Detection Kit (catalog # 6018).  For convenience, the same sample preparations may be used for both the Collagen Detection ELISA Kits and the Sirius Red Collagen Detection Kit.
KIT COMPONENTS
Item Quantity Amount Storage
Standard - Bovine Type I Collagen (90621) 1 vial 0.5 mg/ml, 1 ml -20°C
Sirius Red Solution (90622) 1 bottle 50 ml -20°C
Washing Solution (90623) 1 bottle 50 ml -20°C/*
Extraction Solution (90624) 1 bottle 30 ml -20°C/*
0.5M Acetic Acid (10X Acetic Acid) (90625) 1 bottle 20 ml -20°C/*
96-Well Plate (9026) 1 each 8-well strips x 12 -20°C/*
*Washing solution, extraction solution, 0.5M acetic acid, and the plate can also be stored at room temperature.
Concentrating Solution (50 ml, Catalog # 90626) for cell culture media samples is NOT included.  Please contact Customer Service (support@
chondrex.com) to place an order.
SAMPLE PREPARATION
Tissue specimens and cultured cells can be used; however, solid samples must be solubilized for this assay.  In addition, culture media samples 
may require a concentration process (please see page 3 of this protocol).  Also, heat-denatured collagen tends to have a lower binding affinity 
for sirius red, resulting in underestimated values.
Depending on the solubilization method, these soluble collagen samples can be used:
1) Salt soluble collagen (0.15M NaCl in 0.1M Tris-HCl, pH 7.4)
2) Acid soluble collagen (0.05M acetic acid)
3) Pepsin soluble collagen (0.05M acetic acid with pepsin)
We recommend our “Tips For Collagen Solubilization” to prepare pepsin-soluble collagen samples.