MicroVue Serum PYD EIA*

For Research Use Only in the United States.
Not for use in diagnostic procedures.

MicroVue Serum PYD provides a quantitative measure of the excretion of pyridinoline cross-link (Pyd) in serum. Pyridinoline cross-links are an indicator of type I collagen resorption, especially bone collagen.
 

Features & Benefits

Feature	Benefit

Product Specifications

Format	ELISA
Test/kit	96 wells/plate
Sample type	Serum

Performance Characteristics

Method: Competitive
Analyte: Pyridinoline
Specimen: Serum
Specimen Volume: 25 µL post-filtration
LOD*: 0.4 nmol/L
LLOQ*: 0.75 nmol/L
HLOQ*: 12 nmol/L
Precision CVs within-run: 6-15%
Precision CVs between-run: 9-12%
Incubation Time: 20-26 Hours
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the AssayFormat

96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate

Antibody Specificity

Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)

Species Reactivity

Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat 
No reactivity detected with Rabbit

Assay Steps

Spin serum in Spinfilters (included) for 30 minutes
Add 50 µL Reagent 1 to plate
Add 25 µL serum, diluted Standards, diluted Controls
Add 75 µL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 µL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 µL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 µL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter

Ordering Information

Catalog Number	Description
8019	96 Wells/Plate

Product Documentation

Package Insert

SDS

Technical Data Sheet

Technical Bulletin - ELISA Wash Technique