供应商: 联迈生物 数量: 大量 英文名: pYES-DEST52(pYESDEST52) 保质期: 6个月 保存条件: -20℃ 规格: 1ug
质粒名称: pYES-DEST52
其他名称:
质粒大小(bp): 7621
抗性: Ampicillin
质粒类型: Yeast Expression, Saccharomyces cerevisiae
是否病毒: Nonviral
启动子: P-GAL1
稳转或瞬转: Unspecified
组成型或诱导性: Inducible
表达水平:
蛋白标签: V5 (Cterm), His (Cterm)
测序引物:
GenBank号:
其他属性:
Induced by: Galactose
5′ Sequencing 1 Primer: GAL1
5′ Sequencing 1 Primer Sequence: 5’d[AATATACCTCTATACTTTAACGTC]3′
Selectable Marker: URA3
Notes: Gateway destination vector
质粒图谱:
质粒序列:
1 | ACGGATTAGA | AGCCGCCGAG | CGGGTGACAG | CCCTCCGAAG | GAAGACTCTC | 50 |
51 | CTCCGTGCGT | CCTCGTCTTC | ACCGGTCGCG | TTCCTGAAAC | GCAGATGTGC | 100 |
101 | CTCGCGCCGC | ACTGCTCCGA | ACAATAAAGA | TTCTACAATA | CTAGCTTTTA | 150 |
151 | TGGTTATGAA | GAGGAAAAAT | TGGCAGTAAC | CTGGCCCCAC | AAACCTTCAA | 200 |
201 | ATGAACGAAT | CAAATTAACA | ACCATAGGAT | GATAATGCGA | TTAGTTTTTT | 250 |
251 | AGCCTTATTT | CTGGGGTAAT | TAATCAGCGA | AGCGATGATT | TTTGATCTAT | 300 |
301 | TAACAGATAT | ATAAATGCAA | AAACTGCATA | ACCACTTTAA | CTAATACTTT | 350 |
351 | CAACATTTTC | GGTTTGTATT | ACTTCTTATT | CAAATGTAAT | AAAAGTATCA | 400 |
401 | ACAAAAAATT | GTTAATATAC | CTCTATACTT | TAACGTCAAG | GAGAAAAAAC | 450 |
451 | CCCGGATCGG | ACTACTAGCA | GCTGTAATAC | GACTCACTAT | AGGGAATATT | 500 |
501 | AAGCTATCAA | ACAAGTTTGT | ACAAAAAAGC | TGAACGAGAA | ACGTAAAATG | 550 |
551 | ATATAAATAT | CAATATATTA | AATTAGATTT | TGCATAAAAA | ACAGACTACA | 600 |
601 | TAATACTGTA | AAACACAACA | TATCCAGTCA | CTATGGCGGC | CGCGGGTGAT | 650 |
651 | GCTGCCAACT | TAGCGGCCGC | ATTAGGCACC | CCAGGCTTTA | CACTTTATGC | 700 |
701 | TTCCGGCTCG | TATAATGTGT | GGATTTTGAG | TTAGGATCCG | TCGAGATTTT | 750 |
751 | CAGGAGCTAA | GGAAGCTAAA | ATGGAGAAAA | AAATCACTGG | ATATACCACC | 800 |
801 | GTTGATATAT | CCCAATGGCA | TCGTAAAGAA | CATTTTGAGG | CATTTCAGTC | 850 |
851 | AGTTGCTCAA | TGTACCTATA | ACCAGACCGT | TCAGCTGGAT | ATTACGGCCT | 900 |
901 | TTTTAAAGAC | CGTAAAGAAA | AATAAGCACA | AGTTTTATCC | GGCCTTTATT | 950 |
951 | CACATTCTTG | CCCGCCTGAT | GAATGCTCAT | CCGGAATTCC | GTATGGCAAT | 1000 |
1001 | GAAAGACGGT | GAGCTGGTGA | TATGGGATAG | TGTTCACCCT | TGTTACACCG | 1050 |
1051 | TTTTCCATGA | GCAAACTGAA | ACGTTTTCAT | CGCTCTGGAG | TGAATACCAC | 1100 |
1101 | GACGATTTCC | GGCAGTTTCT | ACACATATAT | TCGCAAGATG | TGGCGTGTTA | 1150 |
1151 | CGGTGAAAAC | CTGGCCTATT | TCCCTAAAGG | GTTTATTGAG | AATATGTTTT | 1200 |
1201 | TCGTCTCAGC | CAATCCCTGG | GTGAGTTTCA | CCAGTTTTGA | TTTAAACGTG | 1250 |
1251 | GCCAATATGG | ACAACTTCTT | CGCCCCCGTT | TTCACCATGG | GCAAATATTA | 1300 |
1301 | TACGCAAGGC | GACAAGGTGC | TGATGCCGCT | GGCGATTCAG | GTTCATCATG | 1350 |
1351 | CCGTCTGTGA | TGGCTTCCAT | GTCGGCAGAA | TGCTTAATGA | ATTACAACAG | 1400 |
1401 | TACTGCGATG | AGTGGCAGGG | CGGGGCGTAA | TCTAGAGGAT | CCGGCTTACT | 1450 |
1451 | AAAAGCCAGA | TAACAGTATG | CGTATTTGCG | CGCTGATTTT | TGCGGTATAA | 1500 |
1501 | GAATATATAC | TGATATGTAT | ACCCGAAGTA | TGTCAAAAAG | AGGTGTGCTA | 1550 |
1551 | TGAAGCAGCG | TATTACAGTG | ACAGTTGACA | GCGACAGCTA | TCAGTTGCTC | 1600 |
1601 | AAGGCATATA | TGATGTCAAT | ATCTCCGGTC | TGGTAAGCAC | AACCATGCAG | 1650 |
1651 | AATGAAGCCC | GTCGTCTGCG | TGCCGAACGC | TGGAAAGCGG | AAAATCAGGA | 1700 |
1701 | AGGGATGGCT | GAGGTCGCCC | GGTTTATTGA | AATGAACGGC | TCTTTTGCTG | 1750 |
1751 | ACGAGAACAG | GGACTGGTGA | AATGCAGTTT | AAGGTTTACA | CCTATAAAAG | 1800 |
1801 | AGAGAGCCGT | TATCGTCTGT | TTGTGGATGT | ACAGAGTGAT | ATTATTGACA | 1850 |
1851 | CGCCCGGGCG | ACGGATGGTG | ATCCCCCTGG | CCAGTGCACG | TCTGCTGTCA | 1900 |
1901 | GATAAAGTCT | CCCGTGAACT | TTACCCGGTG | GTGCATATCG | GGGATGAAAG | 1950 |
1951 | CTGGCGCATG | ATGACCACCG | ATATGGCCAG | TGTGCCGGTC | TCCGTTATCG | 2000 |
2001 | GGGAAGAAGT | GGCTGATCTC | AGCCACCGCG | AAAATGACAT | CAAAAACGCC | 2050 |
2051 | ATTAACCTGA | TGTTCTGGGG | AATATAAATG | TCAGGCTCCG | TTATACACAG | 2100 |
2101 | CCAGTCTGCA | GGTCGACCAT | AGTGACTGGA | TATGTTGTGT | TTTACAGTAT | 2150 |
2151 | TATGTAGTCT | GTTTTTTATG | CAAAATCTAA | TTTAATATAT | TGATATTTAT | 2200 |
2201 | ATCATTTTAC | GTTTCTCGTT | CAGCTTTCTT | GTACAAAGTG | GTTCGATCTA | 2250 |
2251 | GAGGGCCCTT | CGAAGGTAAG | CCTATCCCTA | ACCCTCTCCT | CGGTCTCGAT | 2300 |
2301 | TCTACGCGTA | CCGGTCATCA | TCACCATCAC | CATTGAGTTT | AAACCCGCTG | 2350 |
2351 | ATCCTAGAGG | GCCGCATCAT | GTAATTAGTT | ATGTCACGCT | TACATTCACG | 2400 |
2401 | CCCTCCCCCC | ACATCCGCTC | TAACCGAAAA | GGAAGGAGTT | AGACAACCTG | 2450 |
2451 | AAGTCTAGGT | CCCTATTTAT | TTTTTTATAG | TTATGTTAGT | ATTAAGAACG | 2500 |
2501 | TTATTTATAT | TTCAAATTTT | TCTTTTTTTT | CTGTACAGAC | GCGTGTACGC | 2550 |
2551 | ATGTAACATT | ATACTGAAAA | CCTTGCTTGA | GAAGGTTTTG | GGACGCTCGA | 2600 |
2601 | AGGCTTTAAT | TTGCAAGCTG | CGGCCCTGCA | TTAATGAATC | GGCCAACGCG | 2650 |
2651 | CGGGGAGAGG | CGGTTTGCGT | ATTGGGCGCT | CTTCCGCTTC | CTCGCTCACT | 2700 |
2701 | GACTCGCTGC | GCTCGGTCGT | TCGGCTGCGG | CGAGCGGTAT | CAGCTCACTC | 2750 |
2751 | AAAGGCGGTA | ATACGGTTAT | CCACAGAATC | AGGGGATAAC | GCAGGAAAGA | 2800 |
2801 | ACATGTGAGC | AAAAGGCCAG | CAAAAGCCCA | GGAACCGTAA | AAAGGCCGCG | 2850 |
2851 | TTGCTGGCGT | TTTTCCATAG | GCTCCGCCCC | CCTGACGAGC | ATCACAAAAA | 2900 |
2901 | TCGACGCTCA | AGTCAGAGGT | GGCGAAACCC | GACAGGACTA | TAAAGATACC | 2950 |
2951 | AGGCGTTTCC | CCCTGGAAGC | TCCCTCGTGC | GCTCTCCTGT | TCCGACCCTG | 3000 |
3001 | CCGCTTACCG | GATACCTGTC | CGCCTTTCTC | CCTTCGGGAA | GCGTGGCGCT | 3050 |
3051 | TTCTCATAGC | TCACGCTGTA | GGTATCTCAG | TTCGGTGTAG | GTCGTTCGCT | 3100 |
3101 | CCAAGCTGGG | CTGTGTGCAC | GAACCCCCCG | TTCAGCCCGA | CCGCTGCGCC | 3150 |
3151 | TTATCCGGTA | ACTATCGTCT | TGAGTCCAAC | CCGGTAAGAC | ACGACTTATC | 3200 |
3201 | GCCACTGGCA | GCAGCCACTG | GTAACAGGAT | TAGCAGAGCG | AGGTATGTAG | 3250 |
3251 | GCGGTGCTAC | AGAGTTCTTG | AAGTGGTGGC | CTAACTACGG | CTACACTAGA | 3300 |
3301 | AGGACAGTAT | TTGGTATCTG | CGCTCTGCTG | AAGCCAGTTA | CCTTCGGAAA | 3350 |
3351 | AAGAGTTGGT | AGCTCTTGAT | CCGGCAAACA | AACCACCGCT | GGTAGCGGTG | 3400 |
3401 | GTTTTTTTGT | TTGCAAGCAG | CAGATTACGC | GCAGAAAAAA | AGGATCTCAA | 3450 |
3451 | GAAGATCCTT | TGATCTTTTC | TACGGGGTCT | GACGCTCAGT | GGAACGAAAA | 3500 |
3501 | CTCACGTTAA | GGGATTTTGG | TCATGAGATT | ATCAAAAAGG | ATCTTCACCT | 3550 |
3551 | AGATCCTTTT | AAATTAAAAA | TGAAGTTTTA | AATCAATCTA | AAGTATATAT | 3600 |
3601 | GAGTAAACTT | GGTCTGACAG | TTACCAATGC | TTAATCAGTG | AGGCACCTAT | 3650 |
3651 | CTCAGCGATC | TGTCTATTTC | GTTCATCCAT | AGTTGCCTGA | CTCCCCGTCG | 3700 |
3701 | TGTAGATAAC | TACGATACGG | GAGCGCTTAC | CATCTGGCCC | CAGTGCTGCA | 3750 |
3751 | ATGATACCGC | GAGACCCACG | CTCACCGGCT | CCAGATTTAT | CAGCAATAAA | 3800 |
3801 | CCAGCCAGCC | GGAAGGGCCG | AGCGCAGAAG | TGGTCCTGCA | ACTTTATCCG | 3850 |
3851 | CCTCCATCCA | GTCTATTAAT | TGTTGCCGGG | AAGCTAGAGT | AAGTAGTTCG | 3900 |
3901 | CCAGTTAATA | GTTTGCGCAA | CGTTGTTGGC | ATTGCTACAG | GCATCGTGGT | 3950 |
3951 | GTCACTCTCG | TCGTTTGGTA | TGGCTTCATT | CAGCTCCGGT | TCCCAACGAT | 4000 |
4001 | CAAGGCGAGT | TACATGATCC | CCCATGTTGT | GCAAAAAAGC | GGTTAGCTCC | 4050 |
4051 | TTCGGTCCTC | CGATCGTTGT | CAGAAGTAAG | TTGGCCGCAG | TGTTATCACT | 4100 |
4101 | CATGGTTATG | GCAGCACTGC | ATAATTCTCT | TACTGTCATG | CCATCCGTAA | 4150 |
4151 | GATGCTTTTC | TGTGACTGGT | GAGTACTCAA | CCAAGTCATT | CTGAGAATAG | 4200 |
4201 | TGTATGCGGC | GACCGAGTTG | CTCTTGCCCG | GCGTCAATAC | GGGATAATAG | 4250 |
4251 | TGTATCACAT | AGCAGAACTT | TAAAAGTGCT | CATCATTGGA | AAACGTTCTT | 4300 |
4301 | CGGGGCGAAA | ACTCTCAAGG | ATCTTACCGC | TGTTGAGATC | CAGTTCGATG | 4350 |
4351 | TAACCCACTC | GTGCACCCAA | CTGATCTTCA | GCATCTTTTA | CTTTCACCAG | 4400 |
4401 | CGTTTCTGGG | TGAGCAAAAA | CAGGAAGGCA | AAATGCCGCA | AAAAAGGGAA | 4450 |
4451 | TAAGGGCGAC | ACGGAAATGT | TGAATACTCA | TACTCTTCCT | TTTTCAATGG | 4500 |
4501 | GTAATAACTG | ATATAATTAA | ATTGAAGCTC | TAATTTGTGA | GTTTAGTATA | 4550 |
4551 | CATGCATTTA | CTTATAATAC | AGTTTTTTAG | TTTTGCTGGC | CGCATCTTCT | 4600 |
4601 | CAAATATGCT | TCCCAGCCTG | CTTTTCTGTA | ACGTTCACCC | TCTACCTTAG | 4650 |
4651 | CATCCCTTCC | CTTTGCAAAT | AGTCCTCTTC | CAACAATAAT | AATGTCAGAT | 4700 |
4701 | CCTGTAGAGA | CCACATCATC | CACGGTTCTA | TACTGTTGAC | CCAATGCGTC | 4750 |
4751 | TCCCTTGTCA | TCTAAACCCA | CACCGGGTGT | CATAATCAAC | CAATCGTAAC | 4800 |
4801 | CTTCATCTCT | TCCACCCATG | TCTCTTTGAG | CAATAAAGCC | GATAACAAAA | 4850 |
4851 | TCTTTGTCGC | TCTTCGCAAT | GTCAACAGTA | CCCTTAGTAT | ATTCTCCAGT | 4900 |
4901 | AGATAGGGAG | CCCTTGCATG | ACAATTCTGC | TAACATCAAA | AGGCCTCTAG | 4950 |
4951 | GTTCCTTTGT | TACTTCTTCT | GCCGCCTGCT | TCAAACCGCT | AACAATACCT | 5000 |
5001 | GGGCCCACCA | CACCGTGTGC | ATTCGTAATG | TCTGCCCATT | CTGCTATTCT | 5050 |
5051 | GTATACACCC | GCAGAGTACT | GCAATTTGAC | TGTATTACCA | ATGTCAGCAA | 5100 |
5101 | ATTTTCTGTC | TTCGAAGAGT | AAAAAATTGT | ACTTGGCGGA | TAATGCCTTT | 5150 |
5151 | AGCGGCTTAA | CTGTGCCCTC | CATGGAAAAA | TCAGTCAAGA | TATCCACATG | 5200 |
5201 | TGTTTTTAGT | AAACAAATTT | TGGGACCTAA | TGCTTCAACT | AACTCCAGTA | 5250 |
5251 | ATTCCTTGGT | GGTACGAACA | TCCAATGAAG | CACACAAGTT | TGTTTGCTTT | 5300 |
5301 | TCGTGCATGA | TATTAAATAG | CTTGGCAGCA | ACAGGACTAG | GATGAGTAGC | 5350 |
5351 | AGCACGTTCC | TTATATGTAG | CTTTCGACAT | GATTTATCTT | CGTTTCCTGC | 5400 |
5401 | AGGTTTTTGT | TCTGTGCAGT | TGGGTTAAGA | ATACTGGGCA | ATTTCATGTT | 5450 |
5451 | TCTTCAACAC | TACATATGCG | TATATATACC | AATCTAAGTC | TGTGCTCCTT | 5500 |
5501 | CCTTCGTTCT | TCCTTCTGTT | CGGAGATTAC | CGAATCAAAA | AAATTTCAAA | 5550 |
5551 | GAAACCGAAA | TCAAAAAAAA | GAATAAAAAA | AAAATGATGA | ATTGAATTGA | 5600 |
5601 | AAAGCTAGCT | TATCGATGAT | AAGCTGTCAA | AGATGAGAAT | TAATTCCACG | 5650 |
5651 | GACTATAGAC | TATACTAGAT | ACTCCGTCTA | CTGTACGATA | CACTTCCGCT | 5700 |
5701 | CAGGTCCTTG | TCCTTTAACG | AGGCCTTACC | ACTCTTTTGT | TACTCTATTG | 5750 |
5751 | ATCCAGCTCA | GCAAAGGCAG | TGTGATCTAA | GATTCTATCT | TCGCGATGTA | 5800 |
5801 | GTAAAACTAG | CTAGACCGAG | AAAGAGACTA | GAAATGCAAA | AGGCACTTCT | 5850 |
5851 | ACAATGGCTG | CCATCATTAT | TATCCGATGT | GACGCTGCAG | CTTCTCAATG | 5900 |
5901 | ATATTCGAAT | ACGCTTTGAG | GAGATACAGC | CTAATATCCG | ACAAACTGTT | 5950 |
5951 | TTACAGATTT | ACGATCGTAC | TTGTTACCCA | TCATTGAATT | TTGAACATCC | 6000 |
6001 | GAACCTGGGA | GTTTTCCCTG | AAACAGATAG | TATATTTGAA | CCTGTATAAT | 6050 |
6051 | AATATATAGT | CTAGCGCTTT | ACGGAAGACA | ATGTATGTAT | TTCGGTTCCT | 6100 |
6101 | GGAGAAACTA | TTGCATCTAT | TGCATAGGTA | ATCTTGCACG | TCGCATCCCC | 6150 |
6151 | GGTTCATTTT | CTGCGTTTCC | ATCTTGCACT | TCAATAGCAT | ATCTTTGTTA | 6200 |
6201 | ACGAAGCATC | TGTGCTTCAT | TTTGTAGAAC | AAAAATGCAA | CGCGAGAGCG | 6250 |
6251 | CTAATTTTTC | AAACAAAGAA | TCTGAGCTGC | ATTTTTACAG | AACAGAAATG | 6300 |
6301 | CAACGCGAAA | GCGCTATTTT | ACCAACGAAG | AATCTGTGCT | TCATTTTTGT | 6350 |
6351 | AAAACAAAAA | TGCAACGCGA | CGAGAGCGCT | AATTTTTCAA | ACAAAGAATC | 6400 |
6401 | TGAGCTGCAT | TTTTACAGAA | CAGAAATGCA | ACGCGAGAGC | GCTATTTTAC | 6450 |
6451 | CAACAAAGAA | TCTATACTTC | TTTTTTGTTC | TACAAAAATG | CATCCCGAGA | 6500 |
6501 | GCGCTATTTT | TCTAACAAAG | CATCTTAGAT | TACTTTTTTT | CTCCTTTGTG | 6550 |
6551 | CGCTCTATAA | TGCAGTCTCT | TGATAACTTT | TTGCACTGTA | GGTCCGTTAA | 6600 |
6601 | GGTTAGAAGA | AGGCTACTTT | GGTGTCTATT | TTCTCTTCCA | TAAAAAAAGC | 6650 |
6651 | CTGACTCCAC | TTCCCGCGTT | TACTGATTAC | TAGCGAAGCT | GCGGGTGCAT | 6700 |
6701 | TTTTTCAAGA | TAAAGGCATC | CCCGATTATA | TTCTATACCG | ATGTGGATTG | 6750 |
6751 | CGCATACTTT | GTGAACAGAA | AGTGATAGCG | TTGATGATTC | TTCATTGGTC | 6800 |
6801 | AGAAAATTAT | GAACGGTTTC | TTCTATTTTG | TCTCTATATA | CTACGTATAG | 6850 |
6851 | GAAATGTTTA | CATTTTCGTA | TTGTTTTCGA | TTCACTCTAT | GAATAGTTCT | 6900 |
6901 | TACTACAATT | TTTTTGTCTA | AAGAGTAATA | CTAGAGATAA | ACATAAAAAA | 6950 |
6951 | TGTAGAGGTC | GAGTTTAGAT | GCAAGTTCAA | GGAGCGAAAG | GTGGATGGGT | 7000 |
7001 | AGGTTATATA | GGGATATAGC | ACAGAGATAT | ATAGCAAAGA | GATACTTTTG | 7050 |
7051 | AGCAATGTTT | GTGGAAGCGG | TATTCGCAAT | GGGAAGCTCC | ACCCCGGTTG | 7100 |
7101 | ATAATCAGAA | AAGCCCCAAA | AACAGGAAGA | TTGTATAAGC | AAATATTTAA | 7150 |
7151 | ATTGTAAACG | TTAATATTTT | GTTAAAATTC | GCGTTAAATT | TTTGTTAAAT | 7200 |
7201 | CAGCTCATTT | TTTAACGAAT | AGCCCGAAAT | CGGCAAAATC | CCTTATAAAT | 7250 |
7251 | CAAAAGAATA | GACCGAGATA | GGGTTGAGTG | TTGTTCCAGT | TTCCAACAAG | 7300 |
7301 | AGTCCACTAT | TAAAGAACGT | GGACTCCAAC | GTCAAAGGGC | GAAAAAGGGT | 7350 |
7351 | CTATCAGGGC | GATGGCCCAC | TACGTGAACC | ATCACCCTAA | TCAAGTTTTT | 7400 |
7401 | TGGGGTCGAG | GTGCCGTAAA | GCAGTAAATC | GGAAGGGTAA | ACGGATGCCC | 7450 |
7451 | CCATTTAGAG | CTTGACGGGG | AAAGCCGGCG | AACGTGGCGA | GAAAGGAAGG | 7500 |
7501 | GAAGAAAGCG | AAAGGAGCGG | GGGCTAGGGC | GGTGGGAAGT | GTAGGGGTCA | 7550 |
7551 | CGCTGGGCGT | AACCACCACA | CCCGCCGCGC | TTAATGGGGC | GCTACAGGGC | 7600 |
7601 | GCGTGGGGAT | GATCCACTAG | T | 7621 |
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