IL-22 Antagonist Mouse

IL-22 Antagonist MouseCATALOGUE NUMBER

CYT-148

SYNONYMSIL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23.INTRODUCTIONIL-22 is a member of the IL-10 family of regulatory cytokines.Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-beta/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family.DESCRIPTIONInterleukin-22 Antagonist Mouse Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and having a total molecular mass of 16.7 KDa. The Mouse IL-22 Antagonist is purified by proprietary chromatographic techniques.SOURCEEscherichia Coli.PHYSICAL APPEARANCESterile Filtered White lyophilized (freeze-dried) powder.FORMULATIONIL-22 Antagonist Mouse is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.SOLUBILITYIt is recommended to reconstitute the lyophilized Interleukin -22 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.STABILITYLyophilized Interleukin-22 Mouse Antagonist although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL22 Antagonist should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.PURITYGreater than 98.0% as determined by:
(a) Gel filtration chromatography under non-denaturing conditions.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.AMINO ACID SEQUENCEThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Val-Asn.BIOLOGICAL ACTIVITYIL-22 E117A mutant is capable of full inhibition of STAT3 phosphorylation induced by mouse interleukin 22 in HepG cells. Its affinity toward immobilized mIL-22 receptor a1 extracellular domain (mIL-22 Ra1-ECD) or IL-22 binding protein is similar to the non-mutated mouse interleukin 22. Mouse IL-22 antagonist (E117A) has very low agonistic activity in this bioassay.USAGEProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.