供应商： 钰博生物 数量： 大量 英文名： pLVX-Hom-Mem1 保质期： 6个月 保存条件： -20℃ 规格： 1ug/5ug
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pLVX-Hom-Mem1 |
| 质粒类型: | 慢病毒载体；双顺反子载体；信号通路载体；亚细胞定位载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | |
| 启动子: | 限制性内切酶，多克隆位点 |
| 载体大小: | CMV |
| 5' 测序引物及序列: | 8411 bp |
| 3' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 载体标签: | -- |
| 载体抗性: | DmrB |
| 氨苄青霉素 | |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-Hom-Mem1表达DmrB融合蛋白；利用信号受体的二聚化作用研究细胞信号通路；DmrB蛋白加了膜定位信号。 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
注：When cloning your gene of interest into the pLVX-Hom-Mem1 vector, make certain that the N-myr-signal is located on the N-terminus of the DmrB fusion protein. The N‐myr‐signal is NOT functional if localized in the middle or at the C‐terminus of a protein of interest. Your gene must either be cloned into the 3’MCS, or if cloned into the 5’ MCS, which is located upstream of the N‐myr signal, you must include a separate N‐myr signal sequence on the forward primer used to amplify your gene of interest.
载体序列
LOCUS pLVX-Hom-Mem1 8841 bp ds-DNA circular SYN 30-JUL-2013COMMENT Created by Clontech Laboratories Inc. http://www.clontech.comKEYWORDS pLVX-Hom-Mem1 VectorSOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 8841) AUTHORS Clontech Laboratories Inc.FEATURES Location/Qualifiers source 1..8841 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label="5' LTR" misc_feature 681..806 /label="Packaging signal" misc_feature 1303..1536 /label="RRE" misc_feature 2028..2144 /label="cPPT/CTS" enhancer 2201..2504 /label="CMV enhancer" promoter 2505..2708 /label="CMV promoter" misc_feature 2803..2820 /label="5' MCS" misc_feature 2821..2862 /label="N-myr signal" CDS 2869..3189 /label="DmrB" CDS 3196..3516 /label="DmrB" misc_feature 3518..3531 /label="3' MCS" misc_feature 3544..4117 /label="IRES" CDS 4150..4749 /label="PuroR" misc_feature 4763..5351 /label="WPRE" LTR 5559..6192 /label="3' LTR" rep_origin complement(6723..7308) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7479..8339) /label="AmpR" promoter complement(8340..8444) /label="AmpR promoter"ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 2821 atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg cgtccaagtc 2881 gaaaccatta gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc 2941 cattatacag gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa 3001 cctttcaaat tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct 3061 caaatgtccg tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct 3121 accggacatc ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg 3181 aagctcgaga ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc 3241 cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa 3301 gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg 3361 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact 3421 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc 3481 actctcgtct tcgatgtgga gcttctaaaa ctggaaagcg gccgcggatc ctacgtatga 3541 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 3601 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 3661 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 3721 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 3781 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 3841 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 3901 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 3961 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 4021 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca 4081 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga 4141 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 4201 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 4261 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 4321 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 4381 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 4441 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 4501 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 4561 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4621 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 4681 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 4741 ggtgcctaga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac 4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 5161 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 5341 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca 5401 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag 5461 gaggtgggtt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc 5521 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca 5581 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta 5641 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 5701 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt 5761 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 5821 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa 5881 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg 5941 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt 6001 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6061 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6121 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6181 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 6241 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc 6301 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 6361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 6421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 6481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 6541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 6601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 6661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 6721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 6781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 6841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 6901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 7021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 7081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 7141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 7201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 7261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 7321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 7381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 7441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 7501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 7561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 7621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 7681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 7741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 7801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 7861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 7921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 8041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 8101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 8161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 8221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 8281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 8341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 8401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 8461 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta 8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca 8641 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct 8701 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt 8761 gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat 8821 atgtactaca aacttagtag t
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